Identification of Trachelospermum plants and detection of hybrids using RAPD analysis

Abstract

Trachelospermum asiaticum, T. jasminoides var. pubescens and T. jasminoides var. jasminoides are commercially distributed in Japan. In this study, we analyzed these three lineages by RAPD in order to acquire taxon-specific RAPD markers. We found RAPD markers which could be used for identification of three lineages. Using these markers, Trachelospermum plants for landscape collected from nurseries were analyzed and identified. Variegated cultivars were also analyzed and the results suggested their origins. RAPD analysis indicated that non-native T. jasminoides var. pubescens is also commercially distributed in Japan. Furthermore, progenies of cultivated Trachelospermum plants were analyzed by RAPD. The taxon-specific markers indicated the possibility that natural hybridization between T. asiaticum and T. jasminoides var. pubescens, and between native and non-native T. jasminoides var. pubescens occurred.

Development and characterization of 14 microsatellite markers for Indigofera pseudotinctoria (Fabaceae)

“…However, regulation is difficult when nonnative plants belong to the same species as native plants. In Japan, seeds used to vegetate roadsides after maintenance projects are usually imported from China, South Korea, and Taiwan ( Uemachi et al, 2013 ) due to the lower cost of seed collection and transportation. In many cases, plants of the same species differ genetically between regions.…”