Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice

Watanabe, Satoshi; Kumakura, Kazuo; Kato, Hideki; Iyozumi, Hiroyuki; Togawa, Masayuki; Nagayama, Kozo

doi:10.1007/s10327-005-0217-0

Cited by 24 publications

(16 citation statements)

References 24 publications

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“…These fungi grow very fast and have high sporulation capacity and are tolerant to heat. Dominance of Trichoderma in dam soils may provide benefits to growers using dam soils in their farms as several species of Trichoderma have been reported as potential biological control agents (Watanabe et al, 2005;John et al, 2010;Al-Sadi et al, 2015c;Diánez Martínez et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Al-Sadi¹,

Al-Khatri

Nasehi

et al. 2017

IJAB

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The study analyzed fungal diversity in soil trapped behind dams in three arid regions of Oman, in the south-eastern part of the Arabian Peninsula. Physicochemical analysis indicated that soils are of the loamy sand or sandy loam types and they are deficit in some macro and micro elements. Culture-based techniques revealed variation between dam soils in the number and type of phyla, classes, orders, genera and species. Ascomycota was the most dominant phyla in the three samples, contributing to 89% of the total species. Eurotiomycetes, Sordariomycetes and Dothideomycetes were the most common classes in dam soils. Zygomycetes, Pezizomycetes and Oomycetes were also detected, but at lower levels. Fifty fungal taxa were recovered from the three dam soils, with Aspergillus, Penicillium and Trichoderma being the most common genera. Most of the detected species were saprophytes; however some were either plant pathogens or mycotoxin producers, affecting humans directly or indirectly. This study appears to report for the first time 20 fungal species in Oman. It is also the first study from arid regions to characterize high fungal diversity in soil trapped behind dams. The sources of these fungi in dam soils and the implications of fungal diversity are discussed.

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Section: Discussionmentioning

confidence: 99%

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Al-Sadi¹,

Al-Khatri

Nasehi

et al. 2017

IJAB

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“…For scanning electron microscope (SEM) observation, the spores were dehydrated, dried and gold-coated as previously described. 14) For transmission electron microscope (TEM) observation, the samples were dehydrated in an ethanol series (50, 60, 70, 80, 90, 95 and 100%) for 15 min per step, and immersed in propylene oxide for 30 min. Samples were then substituted with a mixture of propylene oxide and Epon 812 (1 : 1) solution for 4 hr.…”

Section: Observation Of the Spores Under Electron Microscopementioning

confidence: 99%

“…13) Successful applications of BCAs as an effective, safe and viable disease control option will be achieved by finding accurate and reliable BCAs against the target pathogens and establishing prompt application methods. Recently, we have developed a bio-pesticide composed of Trichoderma asperellum isolate SKT-1 14) (ϭT. atroviride Karesten SKT-1) 15) that is highly effective against four diseases of rice caused by seedborne pathogens: "Bakanae" disease caused by Gibberella fujikuroi, bacterial seedling blight caused by Burkholderia plantarii, bacterial grain rot caused by B. glumae and bacterial blight caused by Acidovorax avenae subsp.…”

Section: Introductionmentioning

confidence: 99%

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Properties and biological control activities of aerial and submerged spores in Trichoderma asperellum SKT-1

Watanabe

Kato²,

Kumakura

et al. 2006

J. Pestic. Sci.

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Trichoderma asperellum SKT-1, a biocontrol agent that is highly effective against rice seedborne diseases, forms submerged spores by liquid culture. These submerged spores had some common properties with aerial spores, but differed in surface topology and internal organization. Aerial spores, so called conidia, maintained high viability under dry storage conditions, whereas the viability of submerged spores was reduced. The cell wall of submerged spores was thinner than that of aerial spores, and the surface was smooth in contrast to the irregular pyramidal warts of aerial spores. Both submerged and aerial spores were highly effective against "Bakanae" disease by seed-treatment. Therefore, these two-types of spores were suitable for different applications.

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“…However, little is known about the species of Trichoderma associated with the green mold observed on the shiitake mushrooms in Korea, and only five species of Trichoderma (T. citrinoviride, T. harzianum, T. polysporum, T. longibrachiatum and T. viride) were reported (Anonymous, 2009;Kim et al, 2012). In case of Japan, most of Trichoderma species identified by morphological methods or based on internal transcribed spacer (ITS) sequences (Hashioka, 1973;Miyazaki and Tsunoda, 2003;Miyazaki et al, 2009;Tokimoto and Komatsu, 1975;Tokimoto, 1985;Watanabe et al, 2005). The use of phylogenies based on single gene sequences is now generally discredited, especially the ITS regions of the ribosomal RNA genes, because some fungi have been shown to contain paralogous copies of these sequences (O'Donnell et al, 1998;Lieckfeldt and Seifert, 2000).…”

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confidence: 99%

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

Kim¹,

Park²,

Kim³

et al. 2012

The Plant Pathology Journal

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During investigating of shiitake mushroom competitors, 289 isolates of Trichoderma spp. were collected from shiitake mushroom farms in different districts and the Forest Mushroom Research Center of Korea, among which 29 representative strains were selected. Based on the DNA sequences of the rpb2 and tef1 genes and the ITS rDNA, and their morphological characteristics, they were identified as T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, and two undescribed species, Trichoderma spp. 1 and 2, which are considered to be the candidate of new species. Competition tests between Lentinula edodes (Sanjo302) and the Trichoderma species indicated that the six species of Trichoderma were significantly different from each other in terms of their ability to invade the mycelial blocks of shiitake. In both of dual cultures on potato dextrose agar and sawdust media, Trichoderma spp. 1 and 2 strongly invaded the mycelial blocks of shiitake. Our results suggest that the two Trichoderma species may cause potentially serious economic losses in shiitake cultivation of Korea.

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Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice

Cited by 24 publications

References 24 publications

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Properties and biological control activities of aerial and submerged spores in Trichoderma asperellum SKT-1

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

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