Identification of Triticum aestivum L., Triticum spelta L. and Triticum durum DESF. genotypes on the HMW-GS base

Abstract

The main goal of this work was to identify genotypes of three wheat species (Triticum aestivum L., Triticum spelta L., Triticum durum DESF.) on the basis of individual high-molecular-weight glutenin subunits (HMW-GS) and to predict their technological quality. Detection of HMW-GS was carried out by the standard reference method ISTA SDS-PAGE and the Glu-score was calculated according to the catalogue of alleles for HMW-GS. Among the common wheat varieties the highest Glu-score (10) was determined for the cultivars Axis, Istra and Solara. The most frequently occurring HMW-GS in genotypes of Triticum aestivum L. were 0; 7 + 9; 5 + 10. On the other hand, in the spelt wheat the highest frequency of HMW-GS was detected for 2*; 6 + 8; 2 + 12. The Glu-score for Triticum spelta L. genotypes ranged from 6 to 8. Among the Triticum durum DESF. cultivars, up to 71% were characterized by Glu-score 4, which predetermines them for special baking purposes. The most frequent combination of HMW-GS in durum wheat was 0 and 7 + 8. Thus, SDS PAGE of HMW-GS can be used for identification, differentiation and characterization of different species of wheat and for prediction of bread-making quality of wheat.

Polymorphism of proteins in selected slovak winter wheat genotypes using SDS-PAGE

“…These results were consistent with research of Chňapek et al (2004), Oslovičová et al (2010), Gregová and Šliková (2011) and Ali et al (2013). Tahir (2009) in research detected on Glu-A1 loci allele 1 (50%), on Glu-B1 loci were detected 3 alleles named 7 (50%), 7+9 (16.67%) and 20 (33.33%) and on Glu-D1 loci were detected couples of subunits 5+10 (33.33%) and 2+12 (66.67%).…”

“…The least frequent subunits composition (6.6%) were 0, 7+8, 5+10; 0, 7+8, 2+12; 0, 7+9, 2+12; 0, 6+8, 2+12; 1, 7+9, 2+12 and 1, 6+8, 2+12. The similar electrophoretic profiles of winter wheat were determined by authors Chňapek et al (2004), Oslovičová et al (2010), Gregová and Šliková (2011). Results show that on the Glu-A1 loci were determined two HMW-GS named Null and 1 (Figure 2.).…”

Genetic diversity of European cultivars of common wheat (Triticum aestivum L.) based on RAPD and protein markers

“…The possible reason for the higher polymorphism of storage proteins obtained for SDS -PAGE in the present study could be due to relatively high genetic diversity of HMW -GS. (Chňapek et al, 2013(Chňapek et al, , 2014Gregová et al, 2000;Kuťka Hlozáková et al, 2015;Oslovičová et al, 2010;). Some previous studies based on protein profile demonstrated that numbers of cultivars are indistinguishable (Marchylo and LaBerge, 1981;Shewry et al, 1978), because of that Ahmed et al (2010), Fouda et al (2011) and Shehata (2004) used also DNA marker such as RAPD to differentiate wheat genotypes.…”