Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance

Tang, Ting; Huang, Dawei; Zhang, Di; Wu, Yinjian; Murphy, Robert W.; Liu, Feng-song

doi:10.1016/j.cbpb.2011.06.008

Cited by 16 publications

(11 citation statements)

References 40 publications

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“…The expression patterns of the recombinant BnMTL suggested that the cells transformed with the recombinant plasmids pET30a‐BnMTL had a high tolerance to Cd 2+ stress and can be grown well in the concentration of Cd 2+ under 1000 μ m . This is in accordance with an enhanced tolerance to Cd 2+ in recombinant strains expressing metallothionein being demonstrated in Musca domestica , biofuel plant Jatropha curcas , Anabaena sp. and bacterial metagenome .…”

Section: Resultssupporting

confidence: 88%

Analysis of BnMTL, a novel metallothionein‐like protein in the bast fiber crop Ramie (Boehmeria nivea)

Gao

Chen

et al. 2019

FEBS Open Bio

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Ramie ( Boehmeria nivea ) is a perennial herb that is highly tolerant of heavy metals. In the present study, we cloned a novel metallothionein‐like gene from ramie; this gene, termed Bn MTL , encodes a putative 46 amino acid protein with a molecular mass of 4.38 kD a. Analysis using quantitative RT ‐ PCR revealed that cadmium (Cd 2+ ) treatment results in elevated expression of Bn MTL in the roots. We heterologously overexpressed Bn MTL in Escherichia coli cells to examine its binding to Cd 2+ and its possible role in homeostasis. Recombinant E. coli cells expressing Bn MTL exhibited a high tolerance of Cd 2+ stress up to a concentration of 1 m m , and the observed accumulation of Cd 2+ was almost eight‐fold higher than the control. These results demonstrate that Bn MTL (i) is highly expressed in the root following exposure to Cd 2+ and (ii) encodes a typical metallothionein‐like protein with high cadmium‐binding activity.

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Section: Resultssupporting

confidence: 88%

Analysis of BnMTL, a novel metallothionein‐like protein in the bast fiber crop Ramie (Boehmeria nivea)

Gao

Chen

et al. 2019

FEBS Open Bio

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“…These expression profiles provide additional evidence that AmMT plays a role in metal homeostasis and regulation. Similar results were obtained in Musca domestica (Tang et al, ), where transcriptional activation of metallothioneins corresponded in a dose dependent manner to increasing Cd concentrations. Expression of metallothionein genes is also increased when exposed to different doses and dose durations of Cd in the soil in collembolan Folsomia candida (Nakamori et al, ), Pacific oyster Crassostrea gigas (Choi, Jo, & Choi, ) and springtail Orchesella cincta (Sterenborg & Roelofs, ).…”

Section: Discussionsupporting

confidence: 87%

“…When type 2 metallothionein from Avicennia marina was overexpressed in E. coli BL21 as a carboxy‐terminal extension of glutathione‐S‐transferase, the transgenic bacteria showed an increased tolerance to Zn, Cu, Pb and Cd exposure compared to control strains (Huang & Wang, ). Similar studies were conducted in plants (Bilecen et al, ; Quan, Shan, & Bi, ), as well as other insect metallothionein genes: for example bacteria expressing Musca domestica MT1 enhanced cell tolerance to Cd, but MT2 did not (Tang et al, ). Although these results indicate that AmMT has metal scavenging properties, suggesting a role in metal homeostasis and detoxification, they should be interpreted with caution.…”

Section: Discussionmentioning

confidence: 56%

“…Metallothioneins are proteins that play important roles in metal homeostasis and detoxification in a wide‐range of organisms (Perez‐Rafael et al, ). However, there is limited information on metallothioneins in insects that do not belong to the order Diptera (Atanesyan, Günther, Celniker, Georgiev, & Schaffner, ; Tang et al, ) and to date they have not been identified in honey bees or other hymenoptera species.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure

et al. 2019

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Metallothioneins are ubiquitous proteins important in metal homeostasis and detoxification. However, they have not previously been identified in honey bees or other Hymenoptera, where metallothioneins could be of ecophysiological and ecotoxicological significance. Better understanding of the molecular responses to stress induced by toxic metals could contribute to honey bee conservation. In addition, honey bee metallothionein could represent a biomarker for monitoring environmental quality. Here we identify and characterize a metallothionein gene in Apis mellifera (AmMT). AmMT is 1,680 bp long and encodes a 48 amino acids protein with 15 cysteines and no aromatic residues. A metal response element upstream of the start codon, coupled with numerous cis‐regulatory elements indicate the functional context of AmMT. Molecular modelling predicts several transition metal binding sites, and comparative phylogenetic analysis revealed five putative metallothionein proteins in three other hymenoptera species. AmMT was characterized by cloning the full‐length coding sequence of the putative metallothionein. Recombinant AmMT was found to increase metal tolerance upon overexpression in Escherichia coli supplemented with Cd, Cu or Pb. Finally, in laboratory tests on honey bees, gene expression profiles showed a dose‐dependant relationship between Cd, Cu and Pb concentrations present in food and AmMT expression, while field experiments showed induction of AmMT in bees from an industrial site compared to those from an urban area. These studies suggest that AmMT has metal binding properties in agreement with a possible role in metal homeostasis. Further functional and structural characterization of metallothionein in honey bees and other Hymenoptera are necessary.

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“…The third-instar larvae of house fly (Musca domestica) reared on a diet containing CdCl 2 for 24 h showed significant up-regulation of both MdMT1 and MdMT2. Such up-regulations were interpreted as a metal tolerance mechanism (Tang et al, 2011). In most invertebrates studied, MTs are found to be mainly expressed in digestive tissue (Molto et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional response of two metallothionein genes (OcMT1 and OcMT2) and histological changes in Oxya chinensis (Orthoptera: Acridoidea) exposed to three trace metals

Liu

et al. 2015

Chemosphere

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Identification of two metallothionein genes and their roles in stress responses of Musca domestica toward hyperthermy and cadmium tolerance

Cited by 16 publications

References 40 publications

Analysis of BnMTL, a novel metallothionein‐like protein in the bast fiber crop Ramie (Boehmeria nivea)

Analysis of BnMTL, a novel metallothionein‐like protein in the bast fiber crop Ramie (Boehmeria nivea)

Identification of a metallothionein gene in honey bee Apis mellifera and its expression profile in response to Cd, Cu and Pb exposure

Transcriptional response of two metallothionein genes (OcMT1 and OcMT2) and histological changes in Oxya chinensis (Orthoptera: Acridoidea) exposed to three trace metals

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