Transcriptional response of two metallothionein genes (OcMT1 and OcMT2) and histological changes in Oxya chinensis (Orthoptera: Acridoidea) exposed to three trace metals

Liu, Yaoming; Wu, Hao; Yu, Zujiang; Guo, Yongfeng; Zhang, Jianzhen; Zhu, Kun Yan; Ma, Enbo

doi:10.1016/j.chemosphere.2015.06.043

Cited by 10 publications

(1 citation statement)

References 40 publications

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“…The reaction mixture, amounting to 20 µl, consisted of a 5 × mixture (4 µl), RNA (2 µg), and RNase-free water (16 µl). The reaction was carried out at 37°C for 15 min and then at 85°C for 5 s (Liu et al, 2015). The product was stored at 4°C.…”

Section: Extraction Of Total Rna and Reverse Transcriptionmentioning

confidence: 99%

Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori)

Guo-qing

Shen

Ren

et al. 2020

Arch Insect Biochem Physiol

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Superoxide dismutases (SODs) play an essential role in eliminating excess reactive oxygen species and maintaining the redox balance of the immune system. To study the function of BmSOD3 in silkworm, 543-bp full-length complementary DNA-encoding BmSOD3 was cloned from silkworm. The BmSOD3 amino acids were compared to their homologs, and several highly conserved regions were analyzed. We also carried out phylogenetic analyses of the SOD gene. Our results showed that the BmSOD3 gene belonged with the ecCu/Zn SOD gene. The BmSOD3 gene was transformed into the pET28a vector for functional expression in Escherichia coli. The sodium saltpolyacrylamide gel electrophoresis results showed that the molecular weight of recombinant BmSOD3 was about 22 kDa. The recombinant protein BmSOD3 was purified to detect its properties. After purification analyses, the enzyme activity showed Cu/Zn SOD activity, and the specific activity of the purified enzyme was 0.51 U/mg. The BmSOD3 transcripts showed tissue-specific expression in the midgut and malpighian tubule. The immune microarray data for BmSOD3 showed an expression signal that had a strong response to the induction of four pathogens (Bacillus bombyseptieus, Beauveria bassiana, E. coli, and nuclear polyhedrosis virus), particularly after infection for 24 h, which indicates that the BmSOD3 gene plays a key role in

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Section: Extraction Of Total Rna and Reverse Transcriptionmentioning

confidence: 99%

Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori)

Guo-qing

Shen

Ren

et al. 2020

Arch Insect Biochem Physiol

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Metallothionein in Hermetia illucens (Linnaeus, 1758) larvae (Diptera: Stratiomyidae), a potential biomarker for organic waste system

Wang

Gao

Liu

et al. 2017

Environ Sci Pollut Res

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Black soldier fly, Hermetia illucens (Linnaeus, 1758), is an important economic fly as its larvae can be used for recycling organic waste, such as food waste and manure. H. illucens larvae (BSFL) could uptake Cd from substrates and accumulate it inside bodies, which need to be monitored during waste treatment. Metallothionein (MT) usually serve as biomarker because of its role in metal homeostasis, detoxification, and dose response of heavy metals. Therefore, a MT gene was cloned from H. illucens (HIMT) that encoded 40 amino acids with typical cysteine rich features, which had a high sequence identity with other insect MTs. The expression of HIMT and total MT protein was measured in BSFL fed by meals spiked with gradient dose of Cd (0, 5, 50, 500 mg/kg) for 24, 48, 72, and 96 h, respectively. Dose-associated response of HIMT and total MT were found and the possible correlative range of Cd was from 5 to 50 mg/kg. The expression of HIMT might be a potential biomarker for monitoring Cd contamination by H. illucens in terrestrial organic matters, which might further apply in waste transformation system.

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Effect of phenol on embryo development and expression of metallothionein in the sea urchin Hemicentrotus pulcherrimus

Hwang¹,

Lee²,

Ryu³

et al. 2015

Ocean Sci. J.

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Transcriptional response of two metallothionein genes (OcMT1 and OcMT2) and histological changes in Oxya chinensis (Orthoptera: Acridoidea) exposed to three trace metals

Cited by 10 publications

References 40 publications

Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori)

Molecular cloning, expression, and characterization of BmSOD3 in silkworm (Bombyx mori)

Metallothionein in Hermetia illucens (Linnaeus, 1758) larvae (Diptera: Stratiomyidae), a potential biomarker for organic waste system

Effect of phenol on embryo development and expression of metallothionein in the sea urchin Hemicentrotus pulcherrimus

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