2000
DOI: 10.1128/iai.68.10.5595-5602.2000
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Identification of Vaccine Candidates for Experimental Visceral Leishmaniasis by Immunization with Sequential Fractions of a cDNA Expression Library

Abstract: Visceral leishmaniasis caused by the intracellular parasite Leishmania donovani is a significant public health problem in many regions of the world. Because of its large genome and complex biology, developing a vaccine for this pathogen has proved to be a challenging task and, to date, protective recombinant vaccine candidates have not been identified. To tackle this difficult problem, we adopted a reductionist approach with the intention of identifying cDNA sequences in an L. donovani amastigote cDNA library … Show more

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Cited by 87 publications
(48 citation statements)
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“…To test alternative means of antigen delivery, experiments were designed to evaluate the protection potential of these two antigens delivered in a DNA format. This format of antigen delivery has recently been successfully used with several leishmanial antigens (6,15,17,19,32,38,39,43,46,52). To test this system, TSA and LmSTI1 genes were subcloned into the eukaryotic expression vector pcDNA3 for expression under the control of a strong cytomegalovirus (CMV) promoter.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…To test alternative means of antigen delivery, experiments were designed to evaluate the protection potential of these two antigens delivered in a DNA format. This format of antigen delivery has recently been successfully used with several leishmanial antigens (6,15,17,19,32,38,39,43,46,52). To test this system, TSA and LmSTI1 genes were subcloned into the eukaryotic expression vector pcDNA3 for expression under the control of a strong cytomegalovirus (CMV) promoter.…”
Section: Resultsmentioning
confidence: 99%
“…Over the past few years, much progress has been made using antigen-encoding plasmid DNA as a practical and efficient means of antigen delivery (2,11,53). This system has been particularly successful in vaccine experiments against human immunodeficiency virus, tuberculosis, and leishmaniasis (6,15,17,19,30,32,38,39,43,46,52). It is believed that this form of antigen delivery can induce both CD4 (Th1)-and CD8-positive responses to the immunizing antigen (11,33,45).…”
mentioning
confidence: 99%
“…The ideal vaccine would be a pan-Leishmania vaccine including several molecules, preferably conserved among different species. In this regard, it is worthwhile to mention recent studies showing that cocktail and multicomponent DNA vaccines induce solid protection against both cutaneous and visceral leishmaniasis (3,19,21,24).…”
mentioning
confidence: 99%
“…World Health Organization has identified leishmaniasis as a major and increasing public health problem (4). The visceral form of leishmaniasis is fatal if left untreated, with recent epidemics in Sudan and India resulting in over 100,000 deaths (5). Failure of the pentavalent antimonials, the present main form of chemotherapeutic treatment worldwide, is attributed to the emergence of antimonial-resistant Leishmania strains resulting in frequent relapses after treatment (6,7).…”
mentioning
confidence: 99%