Identification of vancomycin-resistant lactic bacteria isolated from humans

Mackey, Tami-Lea A.; Lejeune, V; Janssens, Michéle; Wauters, Georges

doi:10.1128/jcm.31.9.2499-2501.1993

Cited by 14 publications

(3 citation statements)

References 12 publications

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“…This combination of reactions should accurately identify the enterococci but will not identify the other genera of gram-positive cocci. Other investigators have advocated the use of Gram staining, gas formation from glucose broth, hydrolysis of arginine, PYRase production, and Van r to identify the Van r strains of enterococci, leuconostocs, lactobacilli, and pediococci (11). Although these tests are simple, they require 2 days or more for a final reading.…”

Section: Discussionmentioning

confidence: 99%

“…Vancomycin-resistant (Van r ), facultatively anaerobic and aerobic gram-positive cocci, including Enterococcus, Leuconostoc, and Pediococcus species, are being isolated with increasing frequency from patients (1,9,10,13,14). The tests used to identify these three Van r genera are complex and sometimes require several days to complete (6,11,15). Simple and accurate diagnostic tests are needed to shorten the time required to identify Van r bacteria, because patient management methods may differ according to the infecting organism.…”

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confidence: 99%

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Evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci

et al. 1995

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Simple rapid tests for presumptive identification of catalase-negative non-beta-hemolytic cocci (i.e., enterococci, leuconostocs, and pediococci) have not previously been available. Seven hundred thirty-four strains of aerobic and facultatively anaerobic, catalase-negative, non-beta-hemolytic gram-positive cocci were tested for susceptibility to vancomycin (Van s) by a screening procedure and production of leucine aminopeptidase (LAPase) and pyrrolidonylarylamidase (PYRase) in disk tests. Three unique patterns of activity in response to the three disks (30 g of vancomycin, PYRase, and LAPase) can be used to presumptively identify the vancomycin-resistant (Van r) enterococci (Van r and PYRase and LAPase positive), leuconostocs (Van r and PYRase and LAPase negative), and pediococci (Van r , PYRase negative, and LAPase positive). The results indicate that, together with Gram stain characteristics and the catalase test, the vancomycin, LAPase, and PYRase disk tests can be used to presumptively identify Van r strains of enterococci as well as Leuconostoc and Pediococcus strains from human infections.

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Section: Discussionmentioning

confidence: 99%

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Evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci

et al. 1995

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“…In a few species, the locus is only composed of the TCS predicted operon. The first and the third configurations are observed in vancomycin-resistant Lactobacillus species (Mackey et al, 1993;Wilks et al, 2004;Klare et al, 2007;Balandino et al, 2008) except for L. fermentum which displays a variable strain-dependent vancomycin resistance (Rossetti et al, 2009). The second configuration is only observed in the vancomycinsusceptible species.…”

Section: Predictive Analysis Of Operon Cwars-ldca and Polar Effect Ofmentioning

confidence: 96%

Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei

et al. 2020

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The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the gut. The genes cwaR and cwaS encode a predicted two-component system (TCS) and ldcA a predicted D-alanyl-D-alanine carboxypeptidase which is a peptidoglycan (PG) biosynthesis enzyme. We explored the functionality and the physiological role of these three genes, particularly their impact on the bacterial cell wall architecture and on the bacterial adaptation to environmental perturbations in the gut. The functionality of CwaS/R proteins as a TCS has been demonstrated by biochemical analysis. It is involved in the transcriptional regulation of several genes of the PG biosynthesis. Analysis of the muropeptides of PG in mutants allowed us to re-annotate LSEI_0221 as a putative L,D-carboxypeptidase (LdcA). The absence of this protein coincided with a decrease of two surface antigens: LSEI_0020, corresponding to p40 or msp2 whose implication in the host epithelial homeostasis has been recently studied, and LSEI_2029 which has never been functionally characterized. The inactivation of each of these three genes induces susceptibility to antimicrobial peptides (hBD1, hBD2, and CCL20), which could be the main cause of the gut establishment deficiency. Thus, this operon is necessary for the presence of two surface antigens and for a suitable cell wall architecture.

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Bacteremia caused byLeuconostoc cremoris in a patient with severe burn injuries

Becerril

Gómez-Cía

et al. 1997

Eur. J. Clin. Microbiol. Infect. Dis.

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A case of Leuconostoc cremoris bacteremia in a patient with burn wounds over 45% of the body surface is presented. Leuconostoc bacteremia has not previously been reported in burn patients. The predisposing factors in this case were severe burn wounds resulting in immunocompromise, presence of both intravenous and enteral feeding catheters, several surgical interventions and previous treatment with imipenem plus amikacin. The patient was successfully treated with ampicillin 2 g l.v. every 6 hours for 21 days. Infection with Leuconostoc should be suspected when vancomycin-resistant organisms resembling streptococci are isolated.

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Identification of vancomycin-resistant lactic bacteria isolated from humans

Cited by 14 publications

References 12 publications

Evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci

Evaluation of three disk tests for identification of enterococci, leuconostocs, and pediococci

Study of the cwaRS-ldcA Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in Lactobacillus paracasei

Bacteremia caused byLeuconostoc cremoris in a patient with severe burn injuries

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