Identification of various testicular cell populations in pubertal and adult cockerels

Mucksová, Jitka; Brillard, Jean-Pierre; Hejnar, Jiřı́; Poplstein, Martin; Kalina, Jiří; Bakst, M. R.; Yan, Haifeng; Trefil, Pavel

doi:10.1016/j.anireprosci.2008.10.016

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…Early testicular development is crucial to improving the reproductive performance of roosters. From 2 to 15 weeks, the growth and maturation of Sertoli and Leydig cells is the important step in early testicular development in the chicken [ 4 ]. These cells facilitate the development of pluripotent PGCs into spermatogonia, which support the germinal cells throughout the life of the bird [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…From 2 to 15 weeks, the growth and maturation of Sertoli and Leydig cells is an important step in early testicular development in the chicken [ 4 ]. Although n-3/n-6 (omega-3/omega-6) PUFAs are beneficial to male reproduction capacity, especially sperm quality, the appropriate ratio of n-3/n-6 PUFAs for young roosters’ testicular development and reproductive hormones was not known.…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster

et al. 2015

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BackgroundPolyunsaturated fatty acids (PUFAs) are necessary for the body's metabolism, growth and development. Although PUFAs play an important role in the regulation of reproduction, their role in testis development in the rooster is unknown. The present study was conducted to investigate the effects of omega-3/omega-6 (n-3/n-6, PUFAs) ratios on reproductive performance in young breeder roosters. Plasma levels of reproductive hormones, testis development, and reproductive hormone receptor and StAR mRNA expression were also assessed.ResultsAlthough PUFAs (n-3/n-6: 1/4.15) had no significant effect on the testis index (P > 0.05), the spermatogonial development and germ cell layers were increased. Moreover, serum levels of hormones (GnRH, FSH, LH and T) on day 35 were also significantly increased by PUFAs (n-3/n-6: 1/4.15). To investigate whether PUFAs regulate the expression of hormone receptors and StAR, real time-PCR was used to measure GnRHR, FSHR, LHR and StAR mRNA levels. PUFAs significantly increased the mRNA levels of all of these genes.ConclusionsThese results indicate that PUFAs enhance the reproductive performance of young roosters by increasing hormone secretion and function, the latter by up-regulating receptor expression. These findings provide a sound basis for a balanced n-3/n-6 PUFA ratio being beneficial to young rooster reproduction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster

et al. 2015

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“…Testicular FACS analysis was performed as described with modification. 34–36 Briefly, ES-treated (3 mg/kg) and control animals ( n =5 per group) were killed at respective time points (1, 11, 21 and 31 days post treatment) and the testes were washed in Hank’s Balanced Salt Solution and single cell suspension was prepared. Later, the cells were fixed at a dilution of ~5×10 6 cells/ml using 70% chilled ethanol.…”

Section: Methodsmentioning

confidence: 99%

Exposure to Endosulfan can result in male infertility due to testicular atrophy and reduced sperm count

Sebastian

Raghavan

2015

Cell Death Discovery

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Endosulfan (ES) is a widely used organochlorine pesticide and is speculated to be detrimental to human health. However, very little is known about mechanism of its genotoxicity. Using mouse model system, we show that exposure to ES affected physiology and cellular architecture of organs and tissues. Among all organs, damage to testes was extensive and it resulted in death of different testicular-cell populations. We find that the damage in testes resulted in qualitative and quantitative defects during spermatogenesis in a time-dependent manner, increasing epididymal reactive oxygen species levels, affecting sperm chromatin integrity. This further culminated in reduced number of epididymal sperms and actively motile sperms. Finally, we show that ES exposure affected fertility in male but not in female mice. Therefore, we demonstrate that ES exerts pathophysiological changes in mice, induces testicular atrophy, affects spermatogenesis, reduces quantity and vigour of epididymal sperm and leads to infertility in males.

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“…Ratajczak et al [17] were first to report the presence of pluripotent VSELs in adult mice testis by flow cytometry. Several groups have reported the presence of a 'side population' with stem cell characteristics (having a small size and a low scatter) in testicular tissue of cockerels and mice [18][19][20]. These side population (SP) cells have the ability to repopulate germ cell depleted testis when transplanted in adult mice.…”

Section: Introductionmentioning

confidence: 99%

Very Small Embryonic-Like Stem Cells Survive and Restore Spermatogenesis after Busulphan Treatment in Mouse Testis

An¹,

Bhartiya²,

Sriraman³

et al. 2014

J Stem Cell Res Ther

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Study Objectives: Adult mammalian testes harbor a novel population of quiescent, pluripotent, very small embryonic-like stem cells (VSELs) along with spermatogonial stem cells (SSCs). Present study was undertaken to (i) characterize testicular VSELs (ii) investigate differential effect of chemotherapy on VSELs and SSCs and (iii) to restore the differentiation ability of surviving VSELs by providing healthy somatic microenvironment.Methods: Effect of busulphan (25 mg/Kg) was studied on mouse testes. Syngenic Sertoli cells (10 5 cells per testis) and bone marrow derived mesenchymal cells (10 4 cells per testis) were transplanted separately through intertubular route. Effect of niche reconstruction was studied two months later by histology and immuno-localization of germ cell markers MVH and PCNA. Caudal sperm were evaluated for their ability to fertilize oocytes in vitro.Results: VSELs were 2-6 µm in size, SCA-1 + /CD45 -/LIN -, had high nucleo-cytoplasmic ratio and comprised 0.03% of testicular cells whereas SSC specific marker GFRa localized on a distinct, larger cell population. Busulphan selectively destroyed SSCs and other germ cells however, nuclear OCT-4A, Nanog, Sox-2 and SCA-1 positive VSELs (0.06%) survived. Persisting VSELs were unable to differentiate possibly because chemotherapy also affected the 'niche' comprising Sertoli cells. Complete restoration of spermatogenesis was observed two months post transplantation of Sertoli and mesenchymal cells. Transplanted cells formed neo-tubules in the vicinity of surviving tubules and were possibly a source of growth factors essential for VSELs proliferation and differentiation. Both MVH and PCNA showed increased staining in the transplanted group. qRT-PCR studies revealed existence of a meiotic block in busulphan treated testis which was overcome after transplantation. Resulting sperm progressed to epididymis, showed normal motility and ability to fertilize in vitro. Conclusion:Results show that VSELs survive chemotherapy and can restore spermatogenesis in germ cells depleted mice. Results have direct relevance to address fertility issues of cancer survivors.

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Identification of various testicular cell populations in pubertal and adult cockerels

Cited by 7 publications

References 32 publications

Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster

Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster

Exposure to Endosulfan can result in male infertility due to testicular atrophy and reduced sperm count

Very Small Embryonic-Like Stem Cells Survive and Restore Spermatogenesis after Busulphan Treatment in Mouse Testis

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