Identification of vitronectin as a major plasma protein adsorbed on polymer surfaces of different copolymer composition

Bale,; Wohlfahrt, LA; Mosher, DF; Tomasini, Bianca R.; Sutton, RC

doi:10.1182/blood.v74.8.2698.2698

Cited by 112 publications

(1 citation statement)

References 29 publications

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“…Different types of MNPs, including bare particles, showed relatively high-intensity values associated with vitronectin, compared to the low levels observed for particles D and E. Vitronectin is known as a major plasma protein in association with polymer surfaces. Although fibronectin and vitronectin have similar plasma concentrations, studies conducted on various polystyrene-based surfaces have shown that vitronectin has a greater propensity to bind to surfaces [ 58 ]. Although MNPs showed moderate to high values for vitronectin, none of the MNPs were found to adsorb fibronectin.…”

Section: Resultsmentioning

confidence: 99%

Hemocompatibility of β-Cyclodextrin-Modified (Methacryloyloxy)ethyl Phosphorylcholine Coated Magnetic Nanoparticles

Sharaf

Rowe

et al. 2023

Biomolecules

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Adsorbing toxins from the blood to augment membrane-based hemodialysis is an active area of research. Films composed of β-cyclodextrin-co-(methacryloyloxy)ethyl phosphorylcholine (p(PMβCD-co-MPC)) with various monomer ratios were formed on magnetic nanoparticles and characterized. Surface chemistry effects on protein denaturation were evaluated and indicated that unmodified magnetic nanoparticles greatly perturbed the structure of proteins compared to coated particles. Plasma clotting assays were conducted to investigate the stability of plasma in the presence of particles, where a 2:2 monomer ratio yielded the best results for a given total surface area of particles. Total protein adsorption results revealed that modified surfaces exhibited reduced protein adsorption compared to bare particles, and pure MPC showed the lowest adsorption. Immunoblot results showed that fibrinogen, α1-antitrypsin, vitronectin, prekallikrein, antithrombin, albumin, and C3 correlated with film composition. Hemocompatibility testing with whole blood illustrated that the 1:3 ratio of CD to MPC had a negative impact on platelets, as evidenced by the increased activation, reduced response to an agonist, and reduced platelet count. Other formulations had statistically significant effects on platelet activation, but no formulation yielded apparent adverse effects on hemostasis. For the first time, p(PMβCD-co-MPC)-coated MNP were synthesized and their general hemocompatibility assessed.

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Section: Resultsmentioning

confidence: 99%

Hemocompatibility of β-Cyclodextrin-Modified (Methacryloyloxy)ethyl Phosphorylcholine Coated Magnetic Nanoparticles

Sharaf

Rowe

et al. 2023

Biomolecules

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Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates

Kottke‐Marchant

Veenstra

Marchant

1996

J. Biomed. Mater. Res.

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The in vitro coagulant function of human aortic endothelial cells (HAECs) was investigated when grown on a series of polymer surfaces that ranged from hydrophobic to hydrophilic. The polymer interface materials were prepared by radiofrequency plasma polymerization from hexamethyl-disilazane, gamma-butyrolactone, and N-vinyl-2-pyrrolidone and deposited onto tissue culture Permanox. The three plasma polymers were noncytotoxic. When precoated with fibronectin (FN), HAECs on all four polymer surfaces were similar with respect to cell proliferation and coagulant function. Without FN precoating, cell proliferation and spreading increased with increasing surface hydrophilicity. Normalized production of tissue-type plasminogen activator increased with increasing hydrophilicity of the polymers during early incubation times, as did tissue plasminogen activator/plasminogen activator inhibitor-1 ratios. In comparison, normalized von Willebrand factor release decreased on the more hydrophilic surfaces. Thus, both endothelial cell growth and some coagulant/fibrinolytic functions are improved with increasing substrate hydrophilicity.

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Studies on the biocompatibility of materials: Fibroblast reorganization of substratum-bound fibronectin on surfaces varying in wettability

Altankov

Grinnell

Groth³

1996

J. Biomed. Mater. Res.

340

216

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The ability of human fibroblasts to remove and reorganize fibronectin (FN) bound on material surfaces was studied as a novel feature of material surface biocompatibility. Other traditional parameters of biocompatibility analyzed included cell spreading, clustering of fibronectin receptors into focal adhesions, development of stress fibers, and cell growth. Five different materials with surface wettability ranging from hydrophilic (underwater contact angle 25 degrees) to hydrophobic (underwater contact angle 111 degrees) were used, i.e., clean glass (GLASS), aminopropylsilane (APS), octadecylsilane (ODS), polylactate (PL), and silicone (SI). When cells were cultured on these materials in serum-containing medium, formation of FN receptor-rich focal adhesions and actin stress fibers were more evident on the hydrophilic surfaces (GLASS and APS) compared to the hydrophobic ones (PL, ODS, and SI). Cell growth showed a similar pattern, that is, increased cell proliferation with increasing material surface wettability. Preadsorption of FN on the material surfaces increased subsequent cell spreading and cytoskeletal reorganization on hydrophobic surfaces except SI. Removal and reorganization of FN from the material surfaces into extracellular matrixlike structures occurred on GLASS but not on less wettable surfaces, suggesting that this removal/reorganization process may be more sensitive to changes in surface wettability than other parameters of biocompatibility.

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Identification of vitronectin as a major plasma protein adsorbed on polymer surfaces of different copolymer composition

Cited by 112 publications

References 29 publications

Hemocompatibility of β-Cyclodextrin-Modified (Methacryloyloxy)ethyl Phosphorylcholine Coated Magnetic Nanoparticles

Hemocompatibility of β-Cyclodextrin-Modified (Methacryloyloxy)ethyl Phosphorylcholine Coated Magnetic Nanoparticles

Human endothelial cell growth and coagulant function varies with respect to interfacial properties of polymeric substrates

Studies on the biocompatibility of materials: Fibroblast reorganization of substratum-bound fibronectin on surfaces varying in wettability

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