Identification of volatile biomarkers for high-throughput sensing of soft rot and Pythium leak diseases in stored potatoes

Sangjan, Worasit; Marzougui, Afef; Mattinson, D. Scott; Schroeder, Brenda K.; Bates, Austin A.; Khot, Lav R.; Sankaran, Sindhuja

doi:10.1016/j.foodchem.2021.130910

Cited by 12 publications

(5 citation statements)

References 28 publications

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“…In this study, dimethyl disulfide was found in the sample infected with F. sambucinum (Table S1 and Table 3). Apart from terpenes, other fungal origin compounds were found, including hydrocarbons, acids, ketones, and alcohols [7,[50][51][52][53][54]. In the samples inoculated with C. coccodes and R. solani, a methylated derivative of benzene, i.e., 1,2,4-trimethylbenzene was detected, which was not found in other samples, including the control.…”

Section: Colletotrichum Coccodesmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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The feasibility of early disease detection in potato seeds storage monitoring of volatile organic compounds (VOCs) and plant physiological markers was evaluated using 10 fungal and bacterial pathogens of potato in laboratory-scale experiments. Data analysis of HS-SPME-GC-MS revealed 130 compounds released from infected potatoes, including sesquiterpenes, dimethyl disulfide, 1,2,4-trimethylbenzene, 2,6,11-trimethyldodecane, benzothiazole, 3-octanol, and 2-butanol, which may have been associated with the activity of Fusarium sambucinum, Alternaria tenuissima and Pectobacterium carotovorum. In turn, acetic acid was detected in all infected samples. The criteria of selection for volatiles for possible use as incipient disease indicators were discussed in terms of potato physiology. The established physiological markers proved to demonstrate a negative effect of phytopathogens infecting seed potatoes not only on the kinetics of stem and root growth and the development of the entire root system, but also on gas exchange, chlorophyll content in leaves, and yield. The negative effect of phytopathogens on plant growth was dependent on the time of planting after infection. The research also showed different usefulness of VOCs and physiological markers as the indicators of the toxic effect of inoculated phytopathogens at different stages of plant development and their individual organs.

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Section: Colletotrichum Coccodesmentioning

confidence: 99%

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Steglińska

Pielech‐Przybylska²,

Janas³

et al. 2022

Molecules

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“…Some volatiles were common to control potato tubers. n,n-dimethylmethylamine, 1-undecene, acetone and styrene were some of those VOCs, which were also the unique VOCs of sweet potato with soft rot disease caused by Pectobacterium carotovorum [21].…”

Section: Aspergillus Flavus Ethermentioning

confidence: 96%

Spotting of Volatile Signatures through GC-MS Analysis of Bacterial and Fungal Infections in Stored Potatoes (Solanum tuberosum L.)

Kate

Tiwari

Gujar

et al. 2023

Foods

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Potatoes inoculated with Pectobacterium carotovorum spp., Aspergillus flavus and Aspergillus niger, along with healthy (control) samples, were stored at different storage temperatures (4 ± 1 °C, 8 ± 1 °C, 25 ± 1 °C) for three weeks. Volatile organic compounds (VOCs) were mapped using the headspace gas analysis through solid phase micro extraction–gas chromatography–mass spectroscopy every week. The VOC data were arranged into different groups and classified using principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) models. Based on a variable importance in projection (VIP) score > 2 and the heat map, prominent VOCs were identified as 1-butanol and 1-hexanol, which can act as biomarkers for Pectobacter related bacterial spoilage during storage of potatoes in different conditions. Meanwhile, hexadecanoic acid and acetic acid were signature VOCs for A. flavus, and hexadecane, undecane, tetracosane, octadecanoic acid, tridecene and undecene were associated with A. niger. The PLS-DA model performed better at classifying the VOCs of the three different species of infection and the control sample compared to PCA, with high values of R2 (96–99%) and Q2 (0.18–0.65). The model was also found to be reliable for predictability during random permutation test-based validation. This approach can be adopted for fast and accurate diagnosis of pathogenic invasion of potatoes during storage.

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“…These VOCs can now be used as biomarkers in the future for the detection of this post-harvest disease. 94 There are also some VOCs from green leaves, such as hexanal whose emission increases as a defensive response against Botrytis cinerea showing antifungal properties. 95 The detection of hexanal can thus help in finding fungal infections and its appropriate administration can even help in increasing pathogen resistance.…”

Section: Implementation Of Voc Sensing In Different Fieldsmentioning

confidence: 99%

Nanomaterial-based VOC sensing applications and a deep dive into their developmental trends

Rosario,

Singh,

Tiwari

et al. 2024

J. Mater. Chem. A

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Identification of volatile biomarkers for high-throughput sensing of soft rot and Pythium leak diseases in stored potatoes

Cited by 12 publications

References 28 publications

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

Spotting of Volatile Signatures through GC-MS Analysis of Bacterial and Fungal Infections in Stored Potatoes (Solanum tuberosum L.)

Nanomaterial-based VOC sensing applications and a deep dive into their developmental trends

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