The purpose of the study was to select an environmentally friendly plant biopesticide to protect seed potatoes against phytopathogens. The scope included the evaluation of the antimicrobial activities of 22 plant water extracts, 22 water-glycol extracts, and 3 subcritical carbon dioxide extracts using the agar diffusion method against 10 potato phytopathogens. For the most effective extracts, minimal inhibitory concentration (MIC), chemical composition analysis by gas chromatography–mass spectrometry and in situ assays on seed potatoes were performed. Garlic water extract was finally selected as the most effective in phytopathogen growth inhibition, both in vitro and in situ, with MIC values ranging between 6.3–25 mg/mL. 5-Hydroxymethylfurfural was determined to be the main component of this extract (33.24%). Garlic water extract was proposed as a potential biopesticide against potato phytopathogens.

Biological control offers an alternative to chemical pesticides, which are inconsistent with the global trend of “going green”. Biological control includes various approaches, from natural predators to biologically produced molecules. This article focuses on the selection of lactic acid bacteria (LAB) as biological control agents against potato pathogens. The scope included evaluating the antimicrobial activity of 100 LAB strains against ten phytopatogens (Pectobacterium carotovorum, Streptomyces scabiei, Fusarium oxysporum, Fusarium sambucinum, Alternaria solani, Alternaria, tenuissima, Alternaria alternata, Phoma exigua, Rhizoctonia solani, Colletotrichum coccodes) by cross-streak plate method. HPLC determined the metabolic profiles for the most active LAB strains, and lactic acid, acetic acid, propionic acid and ethanol were found in the largest quantities. The strain Lactiplantibacillus plantarum KB2 LAB 03 was finally selected and cultured on supplemented acid whey. After the selection in laboratory tests, the strain KB2 LAB 03 was assessed in situ on seed potatoes against phytopathogens. The test showed a 40–90% reduction of eight potato pathogens infestation; only F. sambucinum and F. oxysporum were not inhibited at all. L. plantarum KB2 LAB 03 was proposed as the potential biocontrol agent for the potato protection against phytopathogens.

In this work, beeswax was used for the first time for finishing polyester/Cotton/Viscose blend fabric and polyester fabric. The aims of the study were: (1) to characterize the composition of beeswax (using Gas Chromatography Mass Spectrometry, GC-MS and 109AgNPET laser desorption/ionization mass spectrometry (LDI MS); (2) to develop a laboratory method for applying beeswax; (3) to assess the antimicrobial activity of beeswax fabrics against bacteria and fungi (AATCC 100–2004 test); and (4) to assess the properties of textiles modified by beeswax. Beeswax was composed of fatty acids, monoacyl esters, glyceride esters and more complex lipids. The bioactivity of modified fabrics was from −0.09 to 1.55. The highest biocidal activity (>1) was obtained for both fabrics against A. niger mold. The beeswax modification process neither affected the morphological structure of the fibers (the wax evenly covered the surface of the fibers) nor their color. The only statistically significant changes observed were in the mechanical properties of the fabrics. The results obtained indicate that modification of fabrics with beeswax may endow them with biocidal properties against molds, which has practical applications, for example, for the prevention of skin mycoses in health and social care facilities.

An increasing trend in protecting plants against phytopathogens is the replacement of chemical pesticides with environmentally acceptable biopreparations. This article focuses on the possible use of yeast Metschnikowia pulcherrima as a biocontrol agent against potato pathogens. The scope included an assessment of the antimicrobial activity of 10 M. pulcherrima isolates against 10 phytopathogens: Fusarium oxysporum, Fusarium sambucinum, Rhizoctonia solani, Alternaria solani, Alternaria, tenuissima, Alternaria alternata, Colletotrichum coccodes, Phoma exigua, Pectobacterium carotovorum, and Streptomyces scabiei, by the agar-well diffusion method. Pulcherrimin formation, enzymatic profiles detected by the API ZYM system, and metabolite formation evaluated by HPLC analysis were conducted for the most active M. pulcherrima isolates. Leucine arylamidase, valine arylamidase, α- and β-glucosidase, and esterases were the most noteworthy in the pattern of activity. In turn, ethanol, glycerol, and organic acids (acetic, succinic, lactic acids) were determined in the largest quantities. The isolate M. pulcherrima TK1 was selected and cultured on supplemented acid whey. An in situ experiment was carried out on the seed potatoes, which showed a 30%–100% reduction in nine phytopathogens; only P. carotovorum was insensitive to yeast treatment. Therefore, M. pulcherrima TK1 was proposed as the potential biological solution for seed potato protection against phytopathogens.

The aim of the study was to analyze the microbiological biodiversity of human foot skin with respect to factors such as age, gender, frequency of foot washing and physical activity, and to select indicator species to be considered when designing textile materials with antimicrobial properties used for sock and insole production. The experiment was carried out on a group of 40 people. The number of microorganisms was determined using culture-dependent methods. Biodiversity was determined using culture followed by genetic identification based on 16S rRNA gene sequencing (bacteria), ITS region (fungi), or using Illumina next-generation sequencing (in a group of eight selected individuals). The total bacterial number on women’s feet was on average 1.0 × 106 CFU/cm2, and was not statistically significantly different than that of men’s feet (mean 1.2 × 105 CFU/cm2). The number of bacteria, in most cases, decreased with age and with increased frequency of physical activity. The number of bacteria increased with diminishing feet-washing frequency; however, statistically significant differences were found between groups. The number of fungi was not significantly different amongst groups. Bacteria belonging to the phyla Firmicutes, Proteobacteria and Actinobacteria constituted the main microorganisms of the foot skin. Ascomycota and Basidiomycota predominated amongst the fungi. The presence of specific species varied in groups depending on the factors mentioned above. Two of the species identified were classified as pathogens (Neisseria flavescens and Aspergillus fumigatus). These findings suggest that it is necessary to extend the list of microorganisms tested on textiles with respect to hygienic properties.