Identification of volatiles from Pinus silvestris attractive for Monochamus galloprovincialis using a SPME-GC/MS platform

Abstract

IntroductionA myriad of volatile organic compounds (VOCs) released by terrestrial vegetation plays an important role in environmental sciences. A thorough chemical identification of these species at the molecular level is essential in various fields, ranging from atmospheric chemistry to ecology of forest ecosystems. In particular, the recognition of VOCs profiles in a context of plant–insect communication is a key issue for the development of forest protection tools.PurposeThis work was aimed at the development of a simple, robust and reliable method for the identification of volatiles emitted from plant materials, which can attract or deter pest insects. Specifically, volatiles emitted from the bark of Pinus sylvestris were studied, which might attract the black pine sawyer beetle Monochamus galloprovincialis—a serious pest of the tree and a vector of a parasitic nematode Bursaphelenchus xylophius.MethodThe volatiles from bark samples were collected using a solid-phase micro-extraction technique, and subsequently analysed by gas-chromatography/mass-spectrometry (GC/MS). The characterisation of the volatile fraction was based on the comparison with data in mass spectral libraries, and in most cases, with the available authentic standards. The identified compounds were screened against the available entomological data to select insect attractors.ResultsThe identified components included terpenes (α-pinene, ∆-3-carene, and para-cymenene), oxygenated terpenes (α-terpineol and verbenone), sesquiterpenes (α-longipinene, longifolene, E-β-farnesene, γ-cadinene and pentadecane), and diterpenes (manoyl oxide and (+)-pimaral). Of these, longifolene and (+)-pimaral are of particular interest as plausible attractors for the M. galloprovincialis beetle that might find application in the construction of insect bait traps.

Field measurements of biogenic volatile organic compounds in the atmosphere by dynamic solid-phase microextraction and portable gas chromatography-mass spectrometry

“…To avoid an additional concentration and focusing step after desorption of analytes from the traditional adsorbent tubes and the need for long sampling lines, BVOCs can be collected by miniaturized microextraction techniques such as solid-phase microextraction (SPME) (e.g. Foudil-Cherif and Szmigielski et al, 2012;Yassaa et al, 2010), needle trap microextraction (NTME) (e.g. Reyes-Garc es et al, 2013) stir bar sorptive extraction (SBSE) (e.g.…”

Characterization of bacterial communities associated with the pine sawyer beetleMonochamus galloprovincialis, the insect vector of the pinewood nematodeBursaphelenchus xylophilus

“…Different species of Monochamus have been described as vectors of the PWN, the most common being M. alternatus in East Asian forestlands (Mamiya, 1983) and M. galloprovincialis in Europe (Sousa et al, 2001;Naves et al, 2007). During spring season, adult insects are attracted by volatile organic components of stressed trees and fresh cut logs for mating and oviposition (Szmigielski et al, 2011). After hatching, the larvae of Monochamus begin constructing a gallery and feeding on woods' cells until reaching the cambial layer of the tree, where they begin pupation (Linit, 1988).…”

Feeding and oviposition preference of the Sakhalin pine sawyerMonochamus saltuarius(Coleoptera: Cerambycidae) for various tree species

“…For M. galloprovincialis, the reproductive potential is also increased when the beetle develops on P. sylvestris compared with P. nigra (Akbulut 2009). Pinus sylvestris, which has volatile organic compounds (VOCs), particularly various terpenes, was attractive to M. galloprovincialis (Szmigielski et al 2011). In this study, M. saltuarius was likely attracted to volatile materials and fed on both P. densiflora and P. koraiensis.…”

“…Pinus sylvestris , which has volatile organic compounds (VOCs), particularly various terpenes, was attractive to M. galloprovincialis (Szmigielski et al. ). In this study, M. saltuarius was likely attracted to volatile materials and fed on both P. densiflora and P. koraiensis .…”