Identification of Volatiles Produced by Cladosporium cladosporioides CL-1, a Fungal Biocontrol Agent That Promotes Plant Growth

Abstract

Certain microbial Volatile Organic Compounds (VOCs) have been reported to enhance the growth and development of plants. The biocontrol fungi, Cladosporium cladosporioides CL-1 significantly improved the growth of tobacco seedlings in vitro when they were co-cultivated without physical contact. SPME Quadrupole GC/MS/MS revealed that CL-1 emited the volatiles α-pinene, (−)-trans-caryophyllene, tetrahydro-2,2,5,5-tetramethylfuran, dehydroaromadendrene, and (+)-sativene. Potential roles of these volatiles in plant growth and development are discussed. Even though there were several fungal VOCs reported in the past that could influence plant growth, their exact mechanisms of action are not fully known. Fungal VOC-mediated plant growth promotion requires in-depth study in order for this technology to be used in large scale for crops, especially those grown under greenhouse conditions.

Characterization of community structure of culturable endophytic fungi in sweet cherry composite trees and their growth-retarding effect against pathogens

“…Davidiella sp., the telomorph of Cladosporium, is cosmopolitan and has been found in association with different substrata and as an endophyte of plants in temperate and antarctic regions [35,38]. Some Cladosporium species are used as biocontrol agents and were also shown to stimulate growth and root development [30]. As observed in our experiments in sweet cherry, Cladosporium is not exclusively localized in roots, but occurs in leaves as well.…”

Strain-specific variation in plant growth promoting volatile organic compounds production by five different Pseudomonas spp. as confirmed by response of Vigna radiata seedlings

“…As in these earlier reports, the current study detected various VOCs by solvent extraction method using different solvents like methanol, hexane, butanol and ethyl acetate. The compounds 2-Butanone, 3-methyl-1-butanol, tridecane, heptadecane, nonadecane, heptacosane and octadecane were reported previously as plant growth promoting VOCs with role in disease resistance (Siddiquee et al 2012;Paul and Park 2013). In this study, we have detected 20 different VOCs from five different Pseudomonas spp.…”

“…The compounds 2‐Butanone, 3‐methyl‐1‐butanol, tridecane, heptadecane, nonadecane, heptacosane and octadecane were reported previously as plant growth promoting VOCs with role in disease resistance (Siddiquee et al . ; Paul and Park ). In this study, we have detected 20 different VOCs from five different Pseudomonas spp.…”

Do volatile compounds produced by Fusarium oxysporum and Verticillium dahliae affect stress tolerance in plants?

“…Bi-partite Petri plate (=I plate), which has been widely used for studying VC-mediated interaction between fungi and plants (Zhang et al 2008; Paul and Park 2013; Bitas et al 2015), was employed for this assessment. One compartment of I plate contained 12 ml half-strength PDA for culturing fungus, and the other compartment had 12 ml Murashige and Skoog (MS) medium (Sigma-Aldrich, St. Louis, MO) supplemented with no NaCl or 100 mM NaCl.…”