Identification of Weed Hosts of <i>Tomato yellow leaf curl virus</i> in Cyprus

Papayiannis, L. C.; Katis, N. I.; Idris, A. M.; Brown, Judith K.

doi:10.1094/pdis-05-10-0346

Cited by 85 publications

(43 citation statements)

References 34 publications

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“…TYLCV is transmitted by whiteflies and can spread rapidly; it is also not transmitted through seed or by mechanical transmission. Severe symptoms such as leaf curling, stunting, and yellowing showed on TYLCVinfected tomato plants, which cause serious production loss in tomato cultivation (Kil et al, 2016;Papayiannis et al, 2010). In addition to tomato, other cultivated plants including pepper (Capsicum species), common bean (Phaseolus vulgaris), cucurbit (Cucumis species) and eustoma (Eustoma grandiflora) have been reported to be TYLCV hosts (Anfoka et al, 2009;Kil et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Tomato yellow leaf curl virus: Diagnosis and metabolites

Soliman¹,

Mohamed²

2018

Afr. J. Biotechnol.

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The existence of Tomato yellow leaf curl virus (TYLCV) was figured out in different locations in AlAhsaa of Saudi Arabia. Polymerase chain reaction (PCR) results of samples collected showed that TYLCV existed in all locations. Using AVcore and ACcore primers, begomoviruses family were detected in symptomatic tomato plants and by using TYv2664 and TYc138 (specific primers for the detection of TYLCV), the results proved that the samples were infected with TYLCV. The lipid-soluble fraction of healthy and infected tomato leaves extract was compared using gas chromatography techniques. A total of 46 compounds were identified in both healthy and virus-infected leaf tissues; among which 37 metabolites were common between both samples and increased or decreased in concentration due to the virus attack. Nevertheless, eight compounds were exclusively detected in the infected samples with only one compound consumed and thus recognized only in the healthy samples. The classifications and roles of the identified metabolites were discussed from the point of view of plant defense mechanisms or virus resistance against plant defense.

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Section: Introductionmentioning

confidence: 99%

Tomato yellow leaf curl virus: Diagnosis and metabolites

Soliman¹,

Mohamed²

2018

Afr. J. Biotechnol.

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“…Other studies involving different crops have also shown the importance of weeds as potential virus reservoirs to infect crops that are economically important and contributing to disease occurrence during the growing season, and also to virus dissemination (Ali et al, 2012;Papayiannis et al, 2011;Papayiannis et al, 2012;Solórzano-Morales et al, 2011;Asala et al, 2014). Additionally, weeds can still withstand drought in the field, and survive in the absence of preferred hosts, becoming an important initial source of virus inoculum, which can be spread not only to commercial crops, but also to infect other weed plants after harvesting periods (Asala et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Weeds as Virus Reservoirs in Watermelon Crops

et al. 2018

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-Watermelon is one of the most important vegetable crops in Brazil, which is grouped among the greatest producers worldwide. Viruses stand out among the most damaging disease agents, which can drastically reduce fruit production. In this context, weeds present in the field can also interfere in crop production, acting as reservoirs for viruses. Thus, this study aimed to investigate virus occurrence in weeds at the main watermelon-growing regions in the State of Tocantins. Viruses identification (e.g. potyviruses: Watermelon mosaic virus -WMV; Papaya ring spot virus -type watermelon -PRSV-W; Zucchini yellow mosaic virus-ZYMV; the cucumovirus Cucumber mosaic virus -CMV, and the orthotospovirus Zucchini lethal chlorosis virus -ZLCV) infecting weeds was performed by serology and confirmed by RT-PCR tests. Serological and molecular test results indicate that Amaranthus spinosus, Nicandra physaloides, Physalis angulata and Heliotropium indicum were infected by at least one virus species. The highest infection rate was represented by ZYMV (52.7%), followed by PRSV-W (22.2%); CMV, WMV, and ZLCV that showed the same infection rate (8.3%) each. Plants of P. angulata were infected by all five viruses, singly or in mixed infection, and represented 50% of the total number of infected samples. The highest virus infection rates, 50% and 44.4%, occurred in weeds collected at Lagoa da Confusão and Formoso do Araguaia, respectively. The results on occurrence and distribution of viruses infecting weeds in watermelon commercial plantations in the State of Tocantins provide important information about the role of weeds as virus reservoirs contribute to the knowledge of the epidemiology of these diseases, and enable a proper weed management aiming at reducing the secondary spreading control of viruses by insect vectors.Keywords: Citrullus lanatus, alternative hosts, Potyvirus, Cucumovirus, Orthotospovirus. RESUMO -A melancia é uma das olerícolas mais importantes do Brasil, situando o país entre os maiores produtores mundiais. Os vírus se destacam como agentes de doenças prejudiciais, que podem reduzir drasticamente a produção de frutos. Plantas daninhas presentes em campos cultivados também podem interferir na produção de hortaliças, atuando como reservatórios de vírus. O presente estudo teve como objetivo determinar a ocorrência de vírus em plantas daninhas nas principais regiões produtoras de melancia do estado de Tocantins. A identificação de vírus (em geral: os potyvírus Watermelon mosaic virus -WMV, Papaya ringspot virus -type watermelon -PRSV-W, e Zucchini yellow mosaic virus -ZYMV; o cucumovírus Cucumber mosaic virus -CMV e o orthotospovírus Zucchini lethal chlorosis virus -ZLCV) foi realizada por meio de testes serológicos e confirmada por RT-PCR. Os resultados sorológicos e moleculares indicaram que as espécies Copyright:This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that th...

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“…Epidemiological sig nificance of weeds in TYLCV transmission is well known, and their role as a virus reservoir during crop-free periods has been docu mented in previous reports (8,23). Surveys for TYLCV weed hosts have been conducted by numerous researchers from different coun tries (2,(10)(11)(12)17,19,22,23) and identified in a wide range of poten tial hosts, most of which were symptomless. However, most of these studies focused on vims detection from field samples; only some have focused on whitefly transmission analyses.…”

Section: Discussionmentioning

confidence: 99%

“…TYLCV is transmitted to host plants by the vector insect, sweet potato whitefly (.Bemisia tabaci) (7). TYLCV-infected tomato plants show symp toms including curling, interveinal yellowing of leaves, and severe stunting (17). Tomato yellow leaf curl disease (TYLCD) can cause severe tomato production loss.…”

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confidence: 99%

“…), common bean (Phaseolus vulgaris), and eustoma (.Eustoma grandiflora) (1,3,14,15,20). A number o f weeds, including S. nigrum, Conyza sumatrensis, and Lamium amplexicaule, have been identified as TYLCV hosts (2,10,12,17). Furthermore, TYLCV has also been detected in many other weed species from families such as Amaranthaceae, Asclepiadaceae, Caparidaceae, Compositae, Cucurbitaceae, Euphorbiaceae, Leguminosae, Malvaceae, Nyctaginaceae, and Solanaceae (10.17,23).…”

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confidence: 99%

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Tomato yellow leaf curl virus Can Overwinter in Stellaria aquatica, a Winter-Hardy TYLCV-Reservoir Weed

et al. 2015

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S. 2015. Tomato yellow leaf curl virus can overwinter in Stellaria aquatica, a winter-hardy TYLCV-reservoir weed. Plant Dis. 99:588-592.Tomato yellow leaf curl virus (TYLCV), one of the most serious plant viruses in tropical and subtropical regions, is transmitted to host plants by the vector insect Bemisia tabaci. In order to control TYLCV, it is important to identify weed hosts for overwintering TYLCV. Stellaria aquatica, a winterhardy weed, was found growing with TYLCV-infected tomato plants in greenhouse production. TYLCV was detected in S. aquatica plants by poly merase chain reaction and Southern blot hybridization analysis. The intergenic region nucleotide sequences amplified from TYLCVinfected tomato plants, TYLCV-viruliferous whiteflies, and S. aquatica were identical. During winter (December to February), TYLCV-viruliferous whiteflies and TYLCV-infected tomato plants were removed or absent from greenhouses. However, S. aquatica plants were observed over a period of 10 months from August to May in such greenhouses, and TYLCV was con sistently detected in some of these plants. To investigate the transmission of TYLCV from TYLCV-infected S. aquatica plants to healthy tomato plants by whiteflies, TYLCV-infected S. aquatica plants were transplanted to pots in cages with nonviruliferous whiteflies and healthy tomato plants. After 4 weeks, tomato plants developed typical TYLCV disease symptoms, and TYLCV was detected in both whiteflies and tomato plants. These results show that 5. aquatica can act as a winter-hardy reservoir for TYLCV, and suggest that this weed could play an important role in over wintering of TYLCV in tomato greenhouses.

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Identification of Weed Hosts of Tomato yellow leaf curl virus in Cyprus

Cited by 85 publications

References 34 publications

Tomato yellow leaf curl virus: Diagnosis and metabolites

Tomato yellow leaf curl virus: Diagnosis and metabolites

Evaluation of Weeds as Virus Reservoirs in Watermelon Crops

Tomato yellow leaf curl virus Can Overwinter in Stellaria aquatica, a Winter-Hardy TYLCV-Reservoir Weed

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