Identification of wheat flour allergens by means of 2-dimensional immunoblotting

Sander, I.; Flagge, A.; Merget, R.; Halder, Thomas; Meyer, Helmut E.; Baur, Xaver

doi:10.1067/mai.2001.113761

Cited by 137 publications

(102 citation statements)

References 25 publications

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“…This protein has been identified as a major allergen of Triticum aestivum (wheat), several fungi, and as a major allergen in rambutan (Nephelium lappaceum)-induced anaphylaxis. [40][41][42] Several other proteins identified in this analysis have also been reported as mold or pollen allergens in previous studies. Phosphoglycerate kinase is a characterized allergen of Candida albicans.…”

Section: Discussionsupporting

confidence: 75%

Characterization of Cannabis Sativa Allergens

Nayak

Green

Sussman

et al. 2013

Journal of Allergy and Clinical Immunology

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Section: Discussionsupporting

confidence: 75%

Characterization of Cannabis Sativa Allergens

Nayak

Green

Sussman

et al. 2013

Journal of Allergy and Clinical Immunology

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“…2C and 2D). Der f 27 is a serpin, which shares significant similarity with serpin allergens (Tri a33 and Gal d 2) from Triticum aestivum and Gallus domesticus (21,22). The current serpin (Der f 27) from DME was demonstrated to bind with serum IgE from patients with allergy to D. farinae (Fig.…”

Section: Analysis Of Dust Mite Allergen Components By 2-de and Igementioning

confidence: 94%

Dermatophagoides farinae Allergens Diversity Identification by Proteomics

Chen

Long

et al. 2013

Molecular & Cellular Proteomics

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The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens. Molecular & Cellular

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“…Saltsoluble proteins including cereal α-amylase inhibitors, peroxidase, thioredoxin, nonspecific lipid transfer protein (LTP), serine proteinase inhibitor, and thaumatin-like protein as well as salt-insoluble proteins consisting of gliadins and glutenins are known to be allergens associated with baker's asthma (1). More than 100 IgE-binding protein spots have been detected in wheat flour and sera from sensitized bakers show not only reactions to many of these antigens but also considerable variability in individual sensitization patterns (2). A wide array of wheat flour proteins, both salt-soluble proteins and prolamins, and particularly members of the α-amylase inhibitor family and ω5-gliadin (in wheat-dependent exercise-induced anaphylaxis), have been linked to food wheat allergy (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

“…More than 100 IgE-binding protein spots have been detected in wheat flour and sera from sensitized bakers show not only reactions to many of these antigens but also considerable variability in individual sensitization patterns (2). A wide array of wheat flour proteins, both salt-soluble proteins and prolamins, and particularly members of the α-amylase inhibitor family and ω5-gliadin (in wheat-dependent exercise-induced anaphylaxis), have been linked to food wheat allergy (2)(3)(4). LTPs have (wheat flour Tri a 14) recently been identified as a relevant allergen associated with both baker's asthma and wheat IgE-mediated food allergy (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

A Baker with Asthma and Wheat Flour Food Allergy

Yılmaz¹,

Mungan²

2017

Asthma Allergy Immunol

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ÖZBazı ortak allerjenleri paylaşmalarına rağmen fırıncı astımı olan ya da buğday unu ile gıda allerjisi olan hastalarda buğday polenine karşı duyarlılık saptanmamaktadır. Aynı şekilde, fırıncı astımı olan hastalar buğday unundan yapılmış ekmeği sorunsuzca tüketebilmektedir. Bu durum duyarlanma yollarının (inhalasyona karşı sindirim) ve allerjenik kaynakların farklılığı (buğday ununa karşı buğdaydan yapılmış gıda ürünleri) gibi nedenlerle açıklanabilmektedir. Fakat her iki tip allerjiden sorumlu olabilen α-amilaz inhibitörleri, lipid transferaz proteinleri ve gliadin gibi bazı buğday allerjenleri olduğu da bilinmektedir. Biz burada, 30 yıldır fırıncı olarak çalışan 45 yaşındaki bir erkek hastada buğday ununa bağlı fırıncı astımı, riniti ve gıda allerjisi gelişen bir olguyu sunuyoruz. ABSTRACTPatients with wheat flour food allergy and with baker's asthma are not sensitised to wheat pollen, despite the fact that they share some common allergens. In the same way, asthmatic bakers tolerate the ingestion of bread. The difference in sensitisation routes (inhalation versus ingestion) and allergenic source (wheat flour versus processed wheat foodstuffs) could explain this fact, although some wheat allergens, like α-amylase inhibitors, lipid transfer proteins and gliadin are implicated in both types of allergy. We report the case of a 45-year-old male who worked in a bakery for 30 years as a baker. He was confirmed to have occupational asthma, rhinitis and food allergy due to wheat flour.Anahtar kelimeler: Fırıncı astımı, buğday allerjenleri, besin allerjisi, lipid transfer proteinleri, alfa-amilaz inhibitörleri, gliadin

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Identification of wheat flour allergens by means of 2-dimensional immunoblotting

Cited by 137 publications

References 25 publications

Characterization of Cannabis Sativa Allergens

Characterization of Cannabis Sativa Allergens

Dermatophagoides farinae Allergens Diversity Identification by Proteomics

A Baker with Asthma and Wheat Flour Food Allergy

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