Identification of Y chromosomal PCR marker and production of a selected strain for molecular sexing in the brown planthopper, <i>Nilaparvata lugens</i>

Kobayashi, Tetsuya; Noda, Hiroaki

doi:10.1002/arch.20173

Cited by 8 publications

(12 citation statements)

References 23 publications

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“…The sex difference of genome size may have been due to variation in sex chromosome numbers. Male L. striatellus and S. furcifera have 29 chromosomes whereas female have 30 chromosomes (Kobayashi and Noda, 2007; Noda, 2009). The female hemipterans, L. striatellus, S. furcifera , and C. liydipennis , had larger genomes than the males.…”

Section: Resultsmentioning

confidence: 99%

“…These planthoppers are the most destructive rice insect pests in Asia (Noda, 2009; Yin et al, 2014). The chromosome numbers of all 3 are 30 except male S. furcifera and male L. striatellus , which have 29 chromosomes (28+XO) (Kobayashi and Noda, 2007; Noda, 2009). This might explain the fact that male S. furcifera and male L. striatellus have a significantly smaller genome size than females.…”

Section: Discussionmentioning

confidence: 99%

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Genome Sizes of Nine Insect Species Determined by Flow Cytometry and k-mer Analysis

Lin

Wang

et al. 2016

Front. Physiol.

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The flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five Hemipterans, and two hymenopterans. Among which, the coleopteran Lissorhoptrus oryzophilus (Kuschel) had the largest genome of 981 Mb. The average genome size was 504 Mb, suggesting that insects have a moderate-size genome. Compared with the insects in other orders, hymenopterans had small genomes, which were averagely about ~200 Mb. We found that the genome sizes of four insect species were different between male and female, showing the organismal complexity of insects. The largest difference occurred in the coconut leaf beetle Brontispa longissima (Gestro). The male coconut leaf beetle had a 111 Mb larger genome than females, which might be due to the chromosome number difference between the sexes. The results indicated that insect invasiveness was not related to genome size. We also determined the genome sizes of the small brown planthopper Laodelphax striatellus (Fallén) and the parasitic wasp Macrocentrus cingulum (Brischke) using k-mer analysis with Illunima Solexa sequencing data. There were slight differences in the results from the two methods. k-mer analysis indicated that the genome size of L. striatellus was 500–700 Mb and that of M. cingulum was ~150 Mb. In all, the genome sizes information presented here should be helpful for designing the genome sequencing strategy when necessary.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome Sizes of Nine Insect Species Determined by Flow Cytometry and k-mer Analysis

Lin

Wang

et al. 2016

Front. Physiol.

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“…As we could not identify the sex of individual nymphs in our mapping populations, we used the Y-specific marker PM3n 63 as a sex-determining locus ( Sex ). In the consensus map, the putative location of PM3n was mapped at the distal end of LG11.…”

Section: Discussionmentioning

confidence: 99%

A Simple Sequence Repeat- and Single-Nucleotide Polymorphism-Based Genetic Linkage Map of the Brown Planthopper, Nilaparvata lugens

et al. 2012

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In this study, we developed the first genetic linkage map for the major rice insect pest, the brown planthopper (BPH, Nilaparvata lugens). The linkage map was constructed by integrating linkage data from two backcross populations derived from three inbred BPH strains. The consensus map consists of 474 simple sequence repeats, 43 single-nucleotide polymorphisms, and 1 sequence-tagged site, for a total of 518 markers at 472 unique positions in 17 linkage groups. The linkage groups cover 1093.9 cM, with an average distance of 2.3 cM between loci. The average number of marker loci per linkage group was 27.8. The sex-linkage group was identified by exploiting X-linked and Y-specific markers. Our linkage map and the newly developed markers used to create it constitute an essential resource and a useful framework for future genetic analyses in BPH.

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“…The DNA concentrations were measured and diluted to 50 ng/ml. Primers for a male-specific sequence (PM3nF: 59-GAGCTGGAGTGTGTT GATGT-39 and PM3nR: 59-CAAGTTGATTGAAACAGACT-39) and primers for a sequence common to both sexes (PM3femaleF: 59-ATCATACCCGCATGTTGGAC-39 and 59-GAATCTGAAGGGAGCTGTGC-39) were used, as previously described (Kobayashi and Noda 2007). PCR was performed on 1 ml genomic DNA in a 25-ml reaction mixture (50 units/ml Taq DNA polymerase, W/Mg 2+ buffer for DNA polymerase [Biocolors, Shanghai, China]; 2 mM dNTPs, [TaKaRa]).…”

Section: Pcr Of the Male-specific Genomic Dna Fragmentmentioning

confidence: 99%

“…5C and D). To further confirm the sex of these insects, genomic DNA was isolated for PCR verification, using the male-specific primers PM3n (Kobayashi and Noda, 2007). Because NlFmd is essential for female development, the female embryos likely died without functional NlFmd, resulting in the male-only offspring.…”

Section: Nlfmd Is Required For Female Embryo Developmentmentioning

confidence: 99%

Identification of a female determinant gene for the sexual determination of a hemipteran insect, the brown planthopper

Zhuo

Zhang

Xie

et al. 2019

Preprint

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The sex determination mechanism for hemipteran species remains poorly understood. During the sex determination of the brown planthopper (BPH), Nilaparvata lugens, one species of Hemiptera, the functions of doublesex (Nldsx) and NlTra-2 (NlTra-2) genes were identified in our previous studies. Here, we identify an upstream gene for Nldsx in the sex determination cascade, NlFmd, which acts as female determinant gene for N. lugens. The sex-specific transcript of NlFmd (NlFmd-F) encodes an arginine/serine-, and proline-rich protein that is essential for female development. The knockdown of NlFmd resulted in the development of pseudomales, with sex-specific alternative Nldsx processing, and maternal RNA interference (RNAi) against NlFmd generates male-only progeny. Moreover, homologous genes for NlFmd have also been identified in two rice planthopper species, the white-backed planthopper (WBPH, Sogotalla furcifera) and the small brown planthopper (SBPH, Laodelphax striatellus), and these genes appear to be involved in the sex determination cascades for these species. Our data suggest that the sex determination cascade in Delphacidae is conserved.

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Identification of Y chromosomal PCR marker and production of a selected strain for molecular sexing in the brown planthopper, Nilaparvata lugens

Cited by 8 publications

References 23 publications

Genome Sizes of Nine Insect Species Determined by Flow Cytometry and k-mer Analysis

Genome Sizes of Nine Insect Species Determined by Flow Cytometry and k-mer Analysis

A Simple Sequence Repeat- and Single-Nucleotide Polymorphism-Based Genetic Linkage Map of the Brown Planthopper, Nilaparvata lugens

Identification of a female determinant gene for the sexual determination of a hemipteran insect, the brown planthopper

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