Identification of α-Glucosidase Inhibitors from Ipomoea alba by Affinity-Directed Fractionation-Mass Spectrometry

Three Novel Biphenanthrene Derivatives and a New Phenylpropanoid Ester from Aerides multiflora and Their α-Glucosidase Inhibitory Activity

“…In general, α -glucosidase enzymes can be obtained from several sources, for example, Saccharomyces cerevisiae , Rattus norvegicus , and GANC-human [ 65 ]. The enzyme derived from the yeast shows approximately 55% sequence homology with that obtained from mammalian sources [ 66 ], and therefore is widely employed in the investigations of natural compounds for α -glucosidase inhibitory potential [ 67 , 68 ].…”

Glucose-6-Phosphatase and α-Glucosidase Inhibitors from Smilax moranensis Roots Identified by Affinity-Directed Fractionation

“…Affinity-directed fractionation, through the use of size exclusion chromatography (SEC) on spin column centrifugation for the purification of proteins of therapeutic interest for screening of compound libraries of natural extracts, was applied in combination with ESI-MS for ligand detection (Siegel 2007;Rosas-Ramírez et al 2020) to identify G6Pase and αglucosidase inhibitors from S. moranensis roots. First, rat hepatic G6Pase was selected as the enzyme and chlorogenic acid as a high-affinity inhibitor (Fig.…”

“…Time-consuming processes for the purification of bioactive natural products limit the performance of a fast and efficient way to identify bioactive molecules in crude extracts, making the isolation of active metabolites laborious. Consequently, it is essential to develop a simple, economical, and efficient method for the analysis of pharmacologically active metabolites, like the affinity-directed fractionation-mass spectrometry methods used in the present investigation (Fan et al 2019;Rosas-Ramírez et al 2020). Mexico ranks first in the world in childhood obesity, second in adults, and sixth in prevalence of diabetes, one of the main causes of blindness, chronic kidney failure, and non-traumatic amputations (Turnbull et al 2019), which complicates their medical care when facing an emerging disease, such as COVID-19 (Pal and Bhansali 2020).…”

Chemistry and biological activity of resin glycosides from Convolvulaceae species

“…Three resin glycosides from A. acuta , acutacosides C–E ( 111–133 ) also showed weak inhibitory activities with the IC 50 values higher than 100 μM 81 . Two resin glycoside, albinosides VI ( 14 ) and VII ( 15 ), were identified to be α‐Glucosidase inhibitors from I. alba by affinity‐directed fractionation‐mass spectrometry 124 . Moreover, twenty‐seven individual resin glycosides from the morning glory family were evaluated for their α‐glucosidase inhibitory potential, and four of them, pescapreins I and V, purginoside II ( 100 ) and purgin III ( 281 ), displayed an inhibitory activity comparable to acarbose.…”

“…Five quamoclinic acid A derivatives were isolated from the seeds of Q. multifda and Q. pennata. 48,83 Multifdin V (123) and quamoclin VII (124) was characterized as non-macrolactone derivative of quamoclinic acid A methyl ester. 48,83 80,89 Their macrolactone sites and acylated sites were similar with those derived from simonic acid A.…”