Identifikasi Dan Isolasi Senyawa Likopen Dari Semangka (Citrullus Lanatus)

Abstract

Lycopene is one of the phytochemical compounds of the carotenoid group, the main producer of red pigments found in watermelons. Watermelon is a round-shaped vines containing vitamin C and vitamin A and has high antioxidant levels. This study aims to identify lycopene compounds in watermelons. Extraction of watermelon flesh by maceration method. Identification of lycopene compounds with UV-Visibel spectrophotometry instrument followed by identification with the TLC method and fractionation of extracts using preparative TLC with chloroform: ethanol (1: 1) solvents, and identification of fractionation results with Fourier Transform Infrared Spectroscopy (FT-IR). The results of the identification of watermelon fruit extract using the TLC method obtained three spots and the results of UV-Vis spectrophotometry showed three peaks namely between 440-520 nm. The identification of fractionation of watermelon extract using FT-IR obtained a constituent group of lycopene structures, but did not show the R-CH = CH-R group that is owned by lycopene, whereas the results of the test with UV-Vis spectrophotometry appearing at 206 and 245 which indicate the presence of other compounds. From the results of the study it can be concluded that the testing of known extracts of watermelon fruit contains lycopene compounds, but the testing of lycopene identification on the results of fractionation of watermelon fruit extracts was not found with certainty the existence of lycopene because the FT-IR results were only shown and contained on the results UV-Vis spectrophotometry was found to be the wavelength of other compounds.keywords: Watermelon, Lycopene, UV-Vis Spectrophotometry, IR Spectrophotometry

The Effect of Giving Red-fleshed Watermelon Juice (Citrullus lanatus (Thunb.)) on Pregnant White Mice (Mus Musculus) Exposed by Monosodium Glutamate (MSG) Orally on the Number and Morphology of Fetus

“…Watermelon is used because this fruit is a source of natural antioxidants such as beta carotene, vitamin C, citrulline, B vitamins especially B1 and B6, lycopene cucurbitasin, triterpenes, sterols and alkaloids as well as minerals such as potassium and magnesium. 15,23,24 In group II, oral administration of monosodium glutamate at dose of 1 g / kgBW did not significantly affect body weight of the mice, number and fetal morphology (body weight, body length, tail length, and head diameter) (p> 0.05). However, the fetus has abnormalities in the form of haemorrhage (all fetuses) and 1 head had low birth weight (LBW).…”

“…Red-fleshed watermelon juice is made in 2 series groups at doses of 1.4 g/kg, 2.8 g/kg and 4.2 g/kg. 15 To get red-fleshed watermelon juice with this dose, namely by separating the pulp from the skin and seeds, then the pulp is weighed as much as 100 grams, then blended until smooth. After the watermelon juice is obtained, the watermelon juice is weighed according to the dosage and it is sufficient with distilled water to 10 ml.…”

“…Lycopene is also a very powerful antioxidant. 15 In pregnant women, serum lipid peroxide levels (which produce malondialdehyde (MDA) are higher than nonpregnant women. Malandialdehyde (MDA) is a product produced by lipid peroxidation.…”

Review Artikel: Pengaruh Diet Semangka Merah (Citrullus lanatus) terhadap VO2Max

“…35 Salah satu fungsi lycopen selain itu mempengaruhi kualitas buah yang dihasilkan adalah seperti sebuah neutralizer radikal bebas dan mengurangi sel kerusakan dalam tubuh. 36 Penggunaan citrulline di nitrat oksida (tidak), sistem yang fungsi sebagai antioksidan dan vasodilator, ini juga tak terpisahkan dari peran lycopene di jaringan selular tingkat. 37 Kandungan nitrat pada akhirnya akan dikonversi menjadi nitrat oksida (NO ) yang berfungsi sebagai vasodilator seperti antihipertensi, antihypertrophic, dan renoprotective agen untuk menjaga kesehatan kardiovaskular.…”

Pengaruh Variasi Wadah Dan Suhu Terhadap Pembusukan Buah Semangka Potong (Citrullus Lanatus.)

“…dianggap adalah salah satu buah yang memiliki kadar likopen tertinggi dibandingkan buah dan sayuran lain (Saeid et al, 2016). Likopen adalah salah satu senyawa fitokimia dari golongan karotenoid penghasil utama pemberi pigmen berwarna merah yang terdapat pada semangka (Monica & Rollando, 2019). Likopen pada industri pangan dapat digunakan sebagai pewarna alami, selain pewarna, likopen juga berfungsi sebagai antioksidan (Sukriadi et al, 2013).…”