Identifikasi dan Karakterisasi Resistensi Antibiotik Terduga Staphylococcus aureus pada Susu Mastitis Subklinis asal Sapi Perah di Kelompok Ternak Sedyo Mulyo, Pakem, Sleman Yogyakarta

Aziz, Fatkhanuddin; Lestari, Fajar Budi; Indarjulianto, Soedarmanto; Fitriana, Fauziah

doi:10.46549/jipvet.v12i1.226

Cited by 4 publications

(1 citation statement)

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“…Prevalensi resistansi antibiotik pada bakteri yang ada dalam susu dan daging telah dilaporkan kejadiannya oleh Januari et al (2019); Normaliska et al (2019); Aziz et al (2022). Hasil laporan oleh Balai Pengujian mutu Produk Peternakan (BPMPP) tahun 2010, penisilin menggunakan metode Kirby-Bauer disk diffusion dengan penentuan standar berdasarkan Clinical and Laboratory Standards Institute (CLSI).…”

Section: Pendahuluanunclassified

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat

Suherman,

Sudarnika,

Purnawarman

2023

Jurnal Sain Veteriner

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Resistansi antibiotik atau sering disebut sebagai pandemi senyap, merupakan satu diantara penyebab masalah kesehatan global yang harus diwaspadai. Susu segar merupakan media pembawa yang mudah terkontaminasi bakteri, salah satunya ialah bakteri E. coli. Antibiotik yang sering dipakai dalam pengobatan terhadap sapi adalah penisilin yaitu antibiotik golongan betalatam yang memiliki kemampuan membunuh bakteri dengan mencegah pembentukan protein dinding sel. Tujuan penelitian ini adalah untuk mendeteksi keberadaan dan prevalensi E. coli pada susu segar, serta menguji resistansinya terhadap antibiotik penisilin. Sebanyak 75 sampel susu kandang dipilih secara acak, berasal dari 6 kelompok ternak sapi perah dan dikumpulkan oleh masing-masing petugas lapang. Uji identifikasi keberadaan E. coli mengacu kepada Standar Nasional Indonesia (SNI) nomor 01-2897-2008 tentang metode pengujian cemaran mikroba pada susu, daging dan telur. Isolat bakteri yang teridentfikasi E. coli dilaukan pengujian resistansi terhadap antibiotik penisilin menggunakan metode Kirby-Bauer disk diffusion dengan penentuan standar berdasarkan Clinical and Laboratory Standards Institute (CLSI). Wawancara terstruktur dilakukan kepada 75 peternak yang diambil sampel susu kandang. Data yang diperoleh dianalisis secara deskriptif. Hasil identifikasi menunjukkan bahwa bakteri E. coli yang terdeteksi pada sampel susu kandang sebanyak 24/75 isolat (32%). Hasil uji resistansi menunjukkan bahwa sebanyak 23/24 isolat E. coli (96%) resistan terhadap antibiotik penisilin. Kesimpulan dari penelitian ini E. coli yang berhasil diisolasi dari susu kandang di wilayah KPSCU telah resistan terhadap antibiotik penisilin, sehingga dibutuhkan penerapan praktik higiene sanitasi, pengawasan penggunaan antibiotik, peningkatan pengetahuan peternak dan petugas kesehatan hewan terkait penggunaan antibiotik di lapangan.

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Section: Pendahuluanunclassified

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat

Suherman,

Sudarnika,

Purnawarman

2023

Jurnal Sain Veteriner

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Prevalence of mastitis in dairy animals in Indonesia: A systematic review and meta-analysis

et al. 2023

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Background and Aim: Mastitis is an important disease that can reduce milk production and farmer income as well as negatively affect human health. This study aimed to summarize dairy mastitis in Indonesia, both subclinical mastitis (SCM) and clinical mastitis (CM), and its prevalence in different provinces, the diagnostic methods, and the animal species. Materials and Methods: Relevant studies on mastitis in dairy animals in Indonesia were obtained from PubMed, Scopus, ProQuest, Google Scholar, and Garuda. The title and abstract were screened for the eligibility of the studies. The full text of the selected studies was assessed and the data were extracted for analysis. To determine the pooled estimate of the prevalence of mastitis, a random-effects model was performed using the “Meta” and “Metaphor” packages in the R software version 4.2.2. The heterogeneity of several characteristics (mastitis type, provinces, animal species, and diagnostic methods) was evaluated through subgroup meta-analysis. Meta-regression analysis was conducted to assess the trend of mastitis prevalence reports over time. Publication bias was evaluated using Egger’s test and a funnel plot. Results: A total of 735 studies were retrieved for the title and abstract screening, which resulted in the final selection of 37 studies with a total of 6050 samples for meta-analysis. The pooled estimate of mastitis prevalence in dairy animals in Indonesia was 59.44% (95% confidence interval [CI], 52.39%–66.49%). Based on mastitis type, SCM had a significantly higher prevalence than CM (58.24% [95% CI, 51.26%–65.23%] vs. 3.31% [95% CI, 1.42%–5.19%]). No significant difference was observed in the analysis of other subgroups. Among provinces, Central Java had the highest prevalence (66.62% [95% CI, 49.37%–83.87%]), whereas Yogyakarta had the lowest (41.77% [95% CI, 14.96%–68.58%]). Based on animal species, cow and goat had a prevalence of 63.42% (95% CI, 55.97%–70.86%) and 44.96% (95% CI, 28.26%–61.66%), respectively. Based on the diagnostic method, the California mastitis test resulted in 60.08% (95% CI, 52.11%–68.06%) and the Institut Pertanian Bogor test, 56.00% (95% CI, 41.20%–70.81%). No significant change in the prevalence of mastitis in Indonesia was observed from 2003 to 2022. Conclusion: This study demonstrates that the pooled estimate of mastitis prevalence in dairy animals in Indonesia is >50%. Based on subgroup analysis, SCM had a higher prevalence than CM; however, the prevalence between provinces, detection methods, and animal species in the 2003–2022 periods was not significantly different. A mastitis control strategy needs to be developed to reduce the prevalence of mastitis and further loss in milk production. Keywords: animals, cow, Indonesia, subclinical mastitis.

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Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

Aziz,

Fitriana,

Setyorini

et al. 2023

J. Ilmu Peternak. dan Vet. Trop. (Journal Trop. Anim. Vet. Sci.

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Staphylococcus aureus (S. aureus) is a pathogenic bacterium with many virulence factors, such as toxins, immunomodulatory factors, and exoenzymes. Hemolysin is one of the essential toxins in the pathogenesis of S. aureus, with the action of lysing erythrocytes and other cells. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin. Eight S. aureus isolates from goat mastitis and raw chicken meat phenotypically evaluated the ability to hemolyze sheep blood. At the same time, genotypic were determinated by detecting the hla and hlb genes. The results showed that most 87.5% (7/8) of S. aureus isolates had β-hemolysis phenotypic. PCR determination showed that all (8/8) isolates were positive for the α-toxin (hla) encoding gene, while half (4/8) of the isolates were positive for the β-toxin (hlb) encoding gene. The use of 5% defibrinated sheep blood agar plates is recommended to determine the hemolytic character of S. aureus tested. Keywords: Character; Hemolysis; S. aureus Abstrak Staphylococcus aureus (S. aureus) merupakan bakteri patogen yang memiliki banyak faktor virulensi seperti toksin, faktor imunomodulator, dan eksoenzim. Hemolisin merupakan salah satu toksin penting dalam patogenesis S. aureus, dengan aksi kerja melisiskan eritrosit dan sel lainnya. Penelitian ini bertujuan untuk mengetahui karakter hemolisa isolat S. aureus asal hewan secara fenotipik dan genotipik. Delapan isolat S. aureus asal susu kambing mastitis dan daging ayam segar dievaluasi secara fenotipik untuk melihat kemampuan menghemolisa darah domba, sedangkan determinasi genotipik dengan deteksi gen hla dan gen hlb. Hasil penelitian menunjukkan mayoritas 87.5% (7/8) isolat S. aureus memiliki fenotipik β-hemolisa. Determinasi PCR menunjukkan 100% (8/8) isolat positif gen penyandi α-toksin (hla), sedangkan 50% (4/8) isolat positif gen penyandi β-toksin (hlb). Penggunaan 5% defibrinated pelat agar darah domba lebih direkomendasikan untuk melihat karakter hemolisa S. aureus yang diuji. Kata kunci: Hemolisa; Karakter; S. aureus

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Identifikasi dan Karakterisasi Resistensi Antibiotik Terduga Staphylococcus aureus pada Susu Mastitis Subklinis asal Sapi Perah di Kelompok Ternak Sedyo Mulyo, Pakem, Sleman Yogyakarta

Cited by 4 publications

References 0 publications

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat

Prevalence of mastitis in dairy animals in Indonesia: A systematic review and meta-analysis

Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

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