IDENTIFIKASI SENYAWA DAN AKTIVITAS ANTIMALARIA IN VIVO EKSTRAK ETIL ASETAT TANAMAN ANTING-ANTING (Acalypha indica L.)

Abstract

ABSTRAKTelah dilakukan penelitian identifikasi ekstrak etil asetat dari tanama Anting-anting (Acalypha indica Linn.) sebagai senyawa antimalaria dan aktivitasnya secara in vivo pada sel parasit malaria P. berghei.Penelitian ini meliputi ekstraksi tanaman anting-anting menggunakan metode ekstraksi maserasi selama 24 jam dengan variasi pelarut yaitu etil asetat, diklorometana, dan petroleum eter. Pengadukkan dibantu dengan shaker selama 3 jam. Ekstrak pekat diuji fitokimia didukung Kromatografi Lapis Tipis, Ekstrak pekat etil asetat dilakukan uji antimalaria in vivo terhadap hewan uji terhadap sel parasit P. berghei. Data derajat parasitemia mencit dianalisis menggunakan program SPSS dengan Uji OneWay ANOVA dan dilanjutkan dengan Uji Tukey.Hasil penelitian menunjukkan adanya senyawa aktif tanin, alkaloid dan steroid pada ekstrak etil asetat.Uji aktivitas antimalaria secara in vivo pada hewan coba didapatkan hasil penghambatan ekstrak etilasetat terhadap pertumbuhan Plasmodium berghei pada dosis 0,01 mg/g bb sebesar 87,19%; pada dosis 0,1mg/g bb sebesar 84,9% dan pada dosis 1mg/g bb sebesar 90,74%. Kata kunci: Anting-anting (Acalypha indica L.), antimalaria in vivo, Plasmodium berghei COMPOUNDS IDENTIFICATION AND IN VIVO ANTIMALARIAL ACTIVITY OF ETHYL ACETATE EXTRACT FROM ANTING-ANTING PLANT (Acalypha indica L.) ABSTRACTResearch of compound identification of ethyl acetate extract from anting-anting plant (Acalypha indica Linn.) and in vivo antimalarial activity test in cell of the malaria parasite P. bergheiThe research consist of extraction of Anting-anting plant was done with extraction maseration method until 24 hours and shakered until 3 hours. Variation of solvents are ethyl acetate, dichloromethane and petroleum ether. Concentrated extract was in vivo antimalarial tested to animal model. Data of mice parasitemia degree was analyzed using SPSS program with OneWay ANOVA Test dan continued with Tukey Test.The phytochemical compounds in each solvent extract are tannin, alkaloid and steroid in ethyl acetate extract. The value of parasite inhibition is 87,19% for dose 0.01 mg/g wb; 84.9% for dose 0.1 mg/ g wb; 90.74% for dose 1 mg/ g wb.

Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae

“…The drying process by air-dried to prevent damage to the content in the leaves due to the sunlight. Stew is one of the simple methods of extracting heat using water as a solvent [11]. The choice of method and solvent is based on the physical and chemical properties of the secondary metabolites to be explored.…”

“…Observation of the life cycle, that is, the test larvae were given extracts able to survive for a certain period of time but could not reach the next stage. Larvae are considered dead if there are no more signs of life, for example, do not move anymore even though stimulated by the movement of water and touched with a stick [11].…”

“…The result showed that alkaloid, tannin, terpenoid, steroid, flavonoid, and saponin found in the stew. The previous study results showed that this plant contains β-sitosterol and daucosterol, saponins, tannins, flavonoids, and essential oils [7]. This plant has been used in traditional medicine as antidiabetic, anti-inflammatory, antioxidant, anticancer, hepatoprotective, antibacterial, wound healing, anthelmintic, and larvicide [8], [9].…”

“…According to Hayati et al, the components contained in this plant are β-sitosterol and daucosterol, saponins, tannins, flavonoids, and essential oils [7]. The community has been used this plant as traditional medicine and the results of the researches prove its efficacy, among others, as antidiabetic, antiinflammatory, antioxidant, anti-poison, anticancer, hepatoprotective, antibacterial, wound healing, anthelmintic, repellent, and larvicide [3], [8], [9], [10].…”

“…The community has been used this plant as traditional medicine and the results of the researches prove its efficacy, among others, as antidiabetic, antiinflammatory, antioxidant, anti-poison, anticancer, hepatoprotective, antibacterial, wound healing, anthelmintic, repellent, and larvicide [3], [8], [9], [10]. Research conducted by Pratiwi et al (2015) shows that the ethyl acetate extract of A. indica Linn. has a larvicidal effect on Aedes aegypti mosquito larvae with an LC 50 of 72.444 ppm [10].…”