Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm

Chang, Cheng; Zhang, H. P.; Feng, J. M.; Yin, B.; Q., H.; X., C.

doi:10.1007/s11032-009-9346-z

Cited by 34 publications

(28 citation statements)

References 31 publications

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“…The Vp-1B locus showed less polymorphism with 92% of the genotypes containing Vp-1Bc and the others Vp-1Bg. However, other alleles previously detected in Chinese and European germplasm were not detected in this set of germplasm Xia et al 2009;Chang et al 2009a, b). Our previous studies also have shown that most genotypes with the Vp-1Bc allele have medium to high PHS resistance, so we may postulate that the CIMMYT wheat trial lines are characterized by medium to higher dormancy.…”

Section: Discussionmentioning

confidence: 53%

Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm

et al. 2011

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Pre-harvest sprouting (PHS) greatly reduces the quality and economic value of wheat grain. In this study, a total of 168 International Maize and Wheat Improvement Center (CIMMYT) wheat germplasm lines were examined to characterize the haplotypes of Vp-1A, Vp-1B and Vp-1D, which are located on the long arms of chromosomes 3A, 3B and 3D, respectively. Among them, five new alleles of Vp-1Aa (the wild allele) were identified on chromosome 3A, and designated as Vp-1Ab, Vp-1Ac, Vp-1Ad, Vp-1Ae and Vp-1Af, respectively. The main difference between Vp-1Aa and the newly identified alleles was in the numbers of CTT repeats located in the third intron, but Vp-1Af also had 6 and 2 bp deletions at positions 2860-2865 bp and 2880-2881 bp, and possessed five SNPs within the same intron region. In the Vp-1B locus, several alleles (Vp-1Ba, Vp-1Bb, Vp-1Bc, Vp-1Bd, Vp-1Be and Vp-1Bf) have already been identified. In the present material only two, the already known allele Vp-1Bc, and a new one, designated as Vp-1Bg, were detected. Compared with Vp-Ba, Vp-1Bg had additional insertion of TCC at position 2372 bp and a 9 bp change from CTGCATC AC to GCATCAGTG at 2417-2425 bp. However, no polymorphism was detected in Vp-1D. The frequencies of Vp-1Aa Vp-1Ab, Vp-1Ac, Vp-1Ad, Vp-1Ae, and Vp-1Af were 65, 10, 11, 4, 5 and 5%, respectively. For Vp-1B, 155 out of the 168 lines were Vp-1Bc; the remaining 13 were Vp-1Bg. Analyses of the germination index (GI) and abscisic acid (ABA) sensitivity showed that genotypes with Vp-1Ab or Vp-1Af showed higher PHS resistance than the ones with other alleles, suggesting that they might be valuable for CIMMYT breeding program or germplasm introduction. The results presented here will underpin the introduction of germplasm from CIMMYT and the improvement of PHS resistance, both in CIMMYT and elsewhere.Peer reviewe

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Section: Discussionmentioning

confidence: 53%

Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm

et al. 2011

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“…У мягкой пшеницы установлен множественный алле-лизм Vp-1-генов (Yang et al, 2007;Xia et al, 2009;Chang et al, 2010;Yang et al, 2014а, b). Периоды покоя семян и УкПП наиболее тесно ассоциированы с рядом аллелей Vp-1B (Xia et al, 2009;Chang et al, 2010), а также с Vp-1A-локусом (Chang et al, 2011).…”

Section: факторы стабильности высокого числа паденияunclassified

“…Периоды покоя семян и УкПП наиболее тесно ассоциированы с рядом аллелей Vp-1B (Xia et al, 2009;Chang et al, 2010), а также с Vp-1A-локусом (Chang et al, 2011). Период покоя семян и УкПП можно улучшить путем встраивания в хромосому пшеницы Vp-1-генов от овсюга (Avena fatua) (McKibbin et al, 2002) или кукурузы (Zea mays) (Huang et al, 2012).…”

Section: факторы стабильности высокого числа паденияunclassified

Approaches to improve wheat grain quality: breeding for falling number

Krupnov¹,

Krupnova²

2015

Vestn. VOGiS

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Agricultural research institute for South-East regions�� Saratov�� russiaFalling number (Fn� reflects the level of al�ha amylase activity in grain. The main factor res�onsible for high α amylase levels and low Fn values is visible or hidden �reharvest s�routing (PHS� under adverse weather conditions. in recent decades�� great �rogress has been made in the understanding of biochemical and molecular �rocesses accom�anying PHS and the genetic control of �reharvest s�routing resistance (PHSr� and Fn�� as well as in the develo�ment of methods of breeding for PHSr and Fn. However�� breeding for these traits and the corres�onding theoretical studies have not yet been �ro�erly develo�ed or reflected in the russian literature. The �ur�ose of the review is to fill this ga�. it illustrates the genetic com�lexity and context de�endence of the PHSr and Fn traits and of major factors im�airing Fn. A s�ecific feature of breeding for high PHSr and Fn is that it involves elimination of late maturity α-amylase (LMA� genoty�es in choosing �arents for crossing and from early hybrid generations; choice of donors of target traits with regard to their genetic diversity; methods of evaluation of varieties and lines for PHSr and Fn under different environmental conditions; determination of F 2 �o�ulation size with regard to combination of PHSr�� Fn�� high �erformance�� grain quality�� and other commercial traits. new methods and a��roaches in breeding o�en new �ros�ects. They include the doubled ha�loid (DH� method�� allowing homozygotes to be obtained from early hybrid generations; and DnA technologies�� which �ermit genetically reasonable selection of PHSr and Fn donors�� yramiding of desirable alleles�� and efficient selection of desired offs�ring using closely lin�ed molecular mar�ers.Key words�� al�ha amylase; �reharvest s�routing resistance; falling number; molecular mar�ers.Число падения (Ч�� отражает уровен�� активности ал��фа-амилазы в зерне/муке и является международным стандартом ка�ества зерна. Чем ниже Ч�� тем ниже ка�ество зерна и его цена. Главные при�ины снижения Ч� -видимое или скрытое преду�оро�ное прорастание (�� в условиях не�лагоприятной погоды. Реша�щу� рол�� в полу�ении ста�ил��но высокого Ч� играет селекция на устой�ивост�� к преду�оро�ному прорастани� (ук�� а также непосредственно на Ч�. В последние десятилетия достигнут �ол��шой прогресс в понимании �иохими�еских и молекулярных процессов�� происходящих при ��; изу�ении генети�еского контроля ук�� и Ч�� в разра�отке методов селекции на ук�� и Ч�. В России селекция на эти признаки и соответству�щие теорети�еские исследования еще не полу�или должного развития и отражения в литературе. �оказаны генети�еская сложност�� и контекст-зависимост�� признаков ук�� и Ч�. Специфика селекции на высокий уровен�� ук�� и Ч� вкл��ает удаление LMA-генотипов (LMA�� late maturity α-amylase� при под�оре родителей для скрещивания и в ранних поколениях ги�ридов�� вы�ор генети�ески разли�ных доноров целевых признаков�� методы оценки сортов и линий на ук�� и Ч� в разных условиях в...

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“…A RIL population covering 157 lines was developed from the cross between Wanxianbaimaizi and Jing411 by single-seed descent (F 2:8 generation), as described previously (Chang et al 2010b). Wanxianbaimaizi (Vp-1Ab) is a white-grained landrace holding typical high SD and PHS tolerance, and Jing411 (Vp-1Ac) is also a white-grained variety but with very weak SD, as shown in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…However, Vp-1B could only explain 24.6-40.7% of the total phenotypic variation of SD in the RIL population (Chang et al 2010b), and consequently, we presumed that two other orthologous Vp1 genes, one each on 3AL and 3DL, also possibly had an association with SD or PHS tolerance. However, other previous research did not consider allelic variations in these two orthologs (Yang et al 2007;Xia et al 2009).…”

Section: Introductionmentioning

confidence: 96%

Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat

et al. 2011

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The allelic variations of Vp-1B have been confirmed to have close association with seed dormancy (SD) and pre-harvest sprouting (PHS) of Chinese wheat in previous research, but little was known regarding whether the alleles of two other orthologs of Vp1 on 3AL (Vp-1A) and 3DL (Vp-1D) are also present and related to these traits. In view of this, 11 primer pairs flanking the whole sequences of these two orthologs were designed to investigate their allelic variations. The results identified six alleles of Vp-1A using the primer pair A17-19 among 81 wheat cultivars and advanced lines, which were designated as Vp-1Aa, Vp-1Ab, Vp-1Ac, Vp-1Ad, Vp-1Ae, and Vp-1Af. Except for Vp-1Ac, the other five alleles were proven novel, but no allelic variation was found in Vp-1D. On sequence analysis of alleles of Vp-1A, five deletions were observed, all occurring in the same region holding many TTC repeats. Of the six alleles detected in this study, four (Vp-1Aa, Vp-1Ac, Vp-1Ae, and Vp-1Af) were generally distributed in varieties exhibiting higher average germination index (GI, range 0.46-0.56) and spike sprouting (SS, range 39.6-49.4%); however, the alleles Vp-1Ab and Vp1Ad were distributed in genotypes carrying higher SD (GI 0.19-0.26) and stronger PHS resistance (SS 12.3-17.2%). On Spearman correlation analysis, the allele Vp-1Ab had significantly negative correlation with GI (-0.479) and SS (-0.542) at the 0.01 level, and the three alleles Vp-1Aa, Vp-1Ac, and Vp-1Ae had significantly positive correlation with GI [0.311 (0.05 level), 0.401 (0.01 level), and 0.294 (0.05 level)] and SS [0.283 (0.05 level), 0.309 (0.05 level), and 0.266 (0.05 level)]. The other alleles, includingVp-1Ad and Vp-1Af, also exhibited correlation, albeit not significant, with these two traits. This negative correlation showed that Vp-1Ab helped to improve SD and PHS tolerance, but Vp-1Aa, Vp-1Ac, and Vp1Ae appeared to exert the opposite effect. To further confirm the association between alleles of Vp-1A and the two traits, a recombinant inbred line (RIL) population with 157 lines was genotyped using the primer pair A17-19, developed from the cross between Wanxianbaimaizi (Vp-1Ab) and Jing411 (Vp-1Ac). General linear model analysis indicated that variation in Vp-1A had a significant (P \ 0.001) association with the two traits, explaining 23.4% of the variation in GI and 16.7% of the variation in SS in the population across three crop seasons.

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Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm

Cited by 34 publications

References 31 publications

Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm

Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm

Approaches to improve wheat grain quality: breeding for falling number

Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat

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