Identifying and Manipulating Giant Vesicles: Review of Recent Approaches

Toyota, Taro; Zhang, Y.

doi:10.3390/mi13050644

Cited by 9 publications

(7 citation statements)

References 120 publications

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“…Figure A examines incorporation of resveratrol and curcumin within giant vesicles (GVs) comprising dioleylphosphatydilcholine (DOPC) and cardiolipin (CL) (98:2 mol ratio), mimicking mitochondrial outer membrane composition . GVs are useful models for cellular membranes in light of their dimensions and low curvatures Figure A reveals efficient uptake of curcumin and resveratrol by the GVs.…”

Section: Resultsmentioning

confidence: 99%

“…78 GVs are useful models for cellular membranes in light of their dimensions and low curvatures. 79 Figure 6A reveals efficient uptake of curcumin and resveratrol by the GVs. Notably, significant fluorescence intensities of resveratrol or curcumin were apparent when the vesicles were incubated with curcumin/resveratrol/ dendrimer NPs (Figure 6A), accounting for the effective delivery of the polyphenols by the amphiphilic dendrimer NPs.…”

Section: Targeting Mitochondria In Cancermentioning

confidence: 99%

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Synergistic Activity of Anticancer Polyphenols Embedded in Amphiphilic Dendrimer Nanoparticles

Ben-Zichri

Meltzer

Lacham-Hartman

et al. 2022

ACS Appl. Polym. Mater.

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Dendritic polymer nanoparticles (NPs) are promising vehicles for drug delivery. Most dendrimer polymer NPs, however, exhibit positive surface charge which make them, in many instances, cytotoxic. We constructed noncationic, amphiphilic dendrimer NPs embedding curcumin and resveratrol, natural polyphenols exhibiting anticancer properties. The curcumin/resveratrol/dendrimer NPs both effectively shielded the embedded polyphenols and facilitated their slow release and, notably, targeted cancer cells. The experimental data trace the cancer cell toxicity of the curcumin/resveratrol/dendrimer NPs to impairment of mitochondrial functions, specifically giving rise to enhanced intracellular calcium release, inhibition of cytochrome c oxidase enzyme activity, decreased mitochondrial membrane potential, and mitochondrial membrane perturbation. Importantly, synergism between the dendrimer-NP-embedded curcumin and resveratrol was observed, as more pronounced cancer cell death and mitochondrial disruption were induced by the curcumin/resveratrol/dendrimer NPs as compared to either the freely dissolved polyphenols or amphiphilic dendrimer NPs incorporating curcumin or resveratrol separately. This work suggests that amphiphilic dendrimer NPs encapsulating curcumin and resveratrol may constitute a promising anticancer therapeutic platform.

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Section: Resultsmentioning

confidence: 99%

Section: Targeting Mitochondria In Cancermentioning

confidence: 99%

Synergistic Activity of Anticancer Polyphenols Embedded in Amphiphilic Dendrimer Nanoparticles

Ben-Zichri

Meltzer

Lacham-Hartman

et al. 2022

ACS Appl. Polym. Mater.

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“…Optical microscopy is the most commonly used method for GUV observation and evaluation. Phase contrast, differential interference contrast, or dark-field microscopy are adopted to observe non-labeled GUVs, whereas GUVs labeled with encapsulated fluorescent molecules or fluorophore-tagged amphiphiles can be observed under an epifluorescence microscope or a confocal laser scanning fluorescence microscope [41]. Note that in the former observation method for non-labeled GUVs, because the inner solution has a higher refractive index than the lipid bilayer membrane and outer solution, the vesicular membrane itself is often difficult to identify.…”

Section: Practical Guide For the Protocol Of W/o Emulsion Template Me...mentioning

confidence: 99%

A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets

2023

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Giant vesicles (GVs), which are closed lipid bilayer membranes with a diameter of more than 1 μm, have attracted attention not only as model cell membranes but also for the construction of artificial cells. For encapsulating water-soluble materials and/or water-dispersible particles or functionalizing membrane proteins and/or other synthesized amphiphiles, giant unilamellar vesicles (GUVs) have been applied in various fields, such as supramolecular chemistry, soft matter physics, life sciences, and bioengineering. In this review, we focus on a preparation technique for GUVs that encapsulate water-soluble materials and/or water-dispersible particles. It is based on the centrifugation of a water-in-oil emulsion layered on water and does not require special equipment other than a centrifuge, which makes it the first choice for laboratory use. Furthermore, we review recent studies on GUV-based artificial cells prepared using this technique and discuss their future applications.

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“…19,20 They have also been tailored as structural models for studying the properties and behaviors of EVs and cells, as well as to construct protocells to explore the origin of life. 21,22 Because of the structural and functional similarity to cells, lipid vesicles are naturally ideal candidates to develop single particle systems for diverse sensing and therapeutic applications. 23,24 Further integration of lipid vesicles with dynamic DNA motifs enhances their functionality, offering unique opportunities to construct smart sensing systems and drug delivery vehicles.…”

Section: Lu Gaomentioning

confidence: 99%