Identifying Lychee (Litchi chinensis Sonn.) Cultivars and their Genetic Relationships Using Intersimple Sequence Repeat (ISSR) Markers

Abstract

<jats:p>There is widespread confusion and uncertainty concerning the identity of lychee cultivars: the same cultivar may be known under different names and different cultivars may appear under the same name. In the present study, the potential of intersimple sequence repeat (ISSR) for the identification of 66 lychee cultivars and accessions and a determination of their genetic relationships was evaluated, using 32 primers containing different simple sequence repeat motifs. Of the 194 bands produced, 124 (64%) were polymorphic. A set of six ISSR primers was sufficient to distinguish all cultivars and accessions. Thus, cultivars which are morphologically very similar and have identical isozyme profiles can be distinguished by ISSR analysis. However, seven pairs of accessions, each considered to be the same cultivar, were found to be identical by ISSR analysis. Nei and Li band-sharing distances and Nei genetic distances were calculated among the cultivars and two similarity dendrograms were generated using the neighbor-joining algorithm. Results showed that the ISSR technique is a valuable tool for identification of lychee cultivars and analysis of their genetic relationships.</jats:p>

An evaluation of the taxonomic distinctness of two Geonoma macrostachys (Arecaceae) varieties based on intersimple sequence repeat (ISSR) variation

“…Our PPB values fell within the range of variability reported in studies using dominant molecular markers to discriminate between taxa at the species level and below (Loo et al ., 1999; Degani et al ., 2003; Schrader & Graves, 2004; Tian et al ., 2004). For example, the number of total and polymorphic RAPD bands (97 and 65, respectively; PPB = 67%) reported in a study of Licuala glabra Griff.…”

Amplified Fragment Length Polymorphism Fingerprinting to Identify Genetic Relatedness among Lychee Cultivars and Markers Associated with Small-seeded Cultivars

“…Some of the cultivars bear distinctly small to rudimentary seed and larger flesh. The morphological features are affected by climate and soil conditions, and this sometimes causes confusion in naming the cultivars (Degani et al, 2003;Huang et al, 2005). Seedlings of certain cultivars are morphologically similar and thus are often misidentified.…”

Characterization of Rambutan Cultivars (Nephelium lappaceum L.) Based on Leaf Morphological and Genetic CharacterCharacterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic

“…The modification included the addition of Sorbitol buffer before CTAB buffer extraction to minimize the high mucus content of the leaves, and also the addition of repeated DNA purification process to separate polysaccharide and other secondary metabolite residues from the DNA samples. Six out of 31 ISSR primers (Degani et al 2003, Hariri 2017, Riupassa 2016 were selected to produce the clearest and showed the highest polymorphic INTRODUCTION Rambutan (Nephelium lappaceum L.) is one of tropical fruit that has a high potential value. It belongs to Sapindaceae which originated from South East Asia primarily in Indonesia and Malaysia (Leenhouts, 1986).…”