Identifying the Genotypes of Hepatitis B Virus (HBV) with DNA Origami Label

Abstract

The hepatitis B virus (HBV) genotyping may profoundly affect the accurate diagnosis and antiviral treatment of viral hepatitis. Existing genotyping methods such as serological, immunological, or molecular testing are still suffered from substandard specificity and low sensitivity in laboratory or clinical application. In a previous study, a set of high-efficiency hybridizable DNA origami-based shape ID probes to target the templates through which genetic variation could be determined in an ultrahigh resolution of atomic force microscopy (AFM) nanomechanical imaging are established. Here, as a further confirmatory research to explore the sensitivity and applicability of this assay, differentially predesigned DNA origami shape ID probes are also developed for precisely HBV genotyping. Through the specific identification of visualized DNA origami nanostructure with clinical HBV DNA samples, the genetic variation information of genotypes can be directly identified under AFM. As a proof-of-concept, five genotype B and six genotype C are detected in 11 HBV-infected patients' blood DNA samples of Han Chinese population in the single-blinded test. The AFM image-based DNA origami shape ID genotyping approach shows high specificity and sensitivity, which could be promising for virus infection diagnosis and precision medicine in the future.

Tetrahedral DNA nanostructures facilitate neural stem cell migration via activating RHOA/ROCK2 signalling pathway

“…DNA materials have been widely investigated with the aid of DNA nanotechnology . DNA materials have shown potential in various biomedical fields, such as anti‐multidrug resistance and disease diagnostics/therapeutics .…”

“…DNA materials have been widely investigated with the aid of DNA nanotechnology . DNA materials have shown potential in various biomedical fields, such as anti‐multidrug resistance and disease diagnostics/therapeutics . Different DNA nanostructures maintain various biological effects; among them, one of the most promising nanomaterials is tetrahedral DNA nanostructures (TDNs) .…”

Challenges and Perspectives of DNA Nanostructures in Biomedicine

“…This approach was later extended to identify the genotype of hepatitis Bv iruses. [73] Thegeneral detection concepts introduced above can also be adapted for the detection of proteins and other biomarkers.T his usually requires the introduction of target-specific aptamers into the DN. [74] Detection of the bound analyte can then be achieved using AFM, [70,75] electrochemistry, [76] fluorimetry, [60] and CD spectroscopy, [77,78] and successful target detection was recently demonstrated even in whole blood.…”

Overview of DNA Self-Assembling: Progresses in Biomedical Applications

“…Another infection with clinical interest due to its severe epidemic impact is the one caused by Hepatitis B virus (HBV). For virus infection diagnosis, different predesigned DNA origami shape ID probes, including cross and triangular nanostructures, with distinct amounts and positions of capture probes were used to identify HBV genotypes under AFM [151,152]. These two DNA-probes showed high specificity and sensitivity towards simultaneous detection of genetic variation in HBV at a single-molecule level.…”

“…The immobilization of DNA nanostructures into gold surfaces have been used, for example, to detect ( e ) exosomes via redox signal, adapted with permission from [145] or ( f ) miRNA by electrochemiluminescent signal, adapted with permission from [147]. Reconfigurable DNA origami allows the detection of ( g ) Zika-specific artificial DNA and RNA, adapted with permission from [149] and ( h ) Hepatitis B genotyping [151,152] in biological samples, adapted with permission from [151,152].…”