Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling

Guan, Guofang; Xiao, He; Chen, Jingjing; B, Li; Tang, Xuxia

doi:10.3892/mmr.2020.11631

Cited by 9 publications

(4 citation statements)

References 31 publications

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“…Among the commonly decreased mRNAs, Cdkn1a, Egr1, and Ctgf are notable hub genes. A study about cisplatin-induced ototoxicity in auditory cells indicated that Cdkn1a (p21) may be involved in hearing impairment or ototoxicity (28). Moreover, the histone deacetylase inhibition can protect hearing against acute ototoxicity; meanwhile, after treatment, the pro-survival gene Cdkn1a (p21) was elevated (29).…”

Section: Discussionmentioning

confidence: 99%

Key genes and potential drugs in age-related hearing loss: transcriptome analysis of cochlear hair cells in old mice

Xiaoran Yang,

Guihua Wang,

Weimin Liu

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Materials and Methods Selection of presbycusis-related datasetsWe selected the GEO datasets in studies of cochlear hair cells using the term "presbycusis". The criteria were that the mice were divided into at least two groups: an old group and a young group, where the old group was at least 12 months of age, and the young group was less than or equal to 9 months. Overall, five datasets were collected: GSE153882, GSE121856, GSE98070, GSE45026, and GSE98071. The former three datasets provide the expression profiles of mRNAs, and the last two datasets provide that of miRNAs. Differentially expressed genesAll the datasets were analyzed with the DESeq2 R package to acquire the differentially expressed genes (DEGs). The fold change (FC) and P-value were obtained for each

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Section: Discussionmentioning

confidence: 99%

Key genes and potential drugs in age-related hearing loss: transcriptome analysis of cochlear hair cells in old mice

Xiaoran Yang,

Guihua Wang,

Weimin Liu

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…The gDNA was harvested by eluting in 100 μl buffer EB while purity was evaluated using the NanoDrop (Thermo Fisher, United States) and gel electrophoresis using 1% agarose. Quantification of gDNA was carried out using the Qubit double-stranded DNA high-CHS kit following the manufacturer’s guidelines (Life Technologies Inc., Carlsbad, CA, United States) ( Guan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

The mobile gene cassette carrying tetracycline resistance genes in Aeromonas veronii strain Ah5S-24 isolated from catfish pond sediments shows similarity with a cassette found in other environmental and foodborne bacteria

Dubey

Ager-Wiick²,

Peng³

et al. 2023

Front. Microbiol.

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Aeromonas veronii is a Gram-negative bacterium ubiquitously found in aquatic environments. It is a foodborne pathogen that causes diarrhea in humans and hemorrhagic septicemia in fish. In the present study, we used whole-genome sequencing (WGS) to evaluate the presence of antimicrobial resistance (AMR) and virulence genes found in A. veronii Ah5S-24 isolated from catfish pond sediments in South-East, United States. We found cphA4, dfrA3, mcr-7.1, valF, blaFOX-7, and blaOXA-12 resistance genes encoded in the chromosome of A. veronii Ah5S-24. We also found the tetracycline tet(E) and tetR genes placed next to the IS5/IS1182 transposase, integrase, and hypothetical proteins that formed as a genetic structure or transposon designated as IS5/IS1182/hp/tet(E)/tetR/hp. BLAST analysis showed that a similar mobile gene cassette (MGC) existed in chromosomes of other bacteria species such as Vibrio parahaemolyticus isolated from retail fish at markets, Aeromonas caviae from human stool and Aeromonas media from a sewage bioreactor. In addition, the IS5/IS1182/hp/tet(E)/tetR/hp cassette was also found in the plasmid of Vibrio alginolyticus isolated from shrimp. As for virulence genes, we found the tap type IV pili (tapA and tapY), polar flagellae (flgA and flgN), lateral flagellae (ifgA and IfgL), and fimbriae (pefC and pefD) genes responsible for motility and adherence. We also found the hemolysin genes (hylII, hylA, and TSH), aerA toxin, biofilm formation, and quorum sensing (LuxS, mshA, and mshQ) genes. However, there were no MGCs encoding virulence genes found in A. veronii AhS5-24. Thus, our findings show that MGCs could play a vital role in the spread of AMR genes between chromosomes and plasmids among bacteria in aquatic environments. Overall, our findings are suggesting that MGCs encoding AMR genes could play a vital role in the spread of resistance acquired from high usage of antimicrobials in aquaculture to animals and humans.

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“…The purity of gDNA was assessed using the NanoDrop (Thermo Fisher, Arbor, Michigan United States) and gel electrophoresis using 1% agarose. Quantification of gDNA was done using the Qubit double-stranded DNA high-CHS kit based on the manufacturer’s instructions (Life Technologies Inc., Carlsbad, CA, United States) ( Guan et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles

et al. 2022

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Aeromonas species are Gram-negative bacteria that infect various living organisms and are ubiquitously found in different aquatic environments. In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii isolated from Indian major carp (Catla catla), Indian carp (Labeo rohita), catfish (Clarias batrachus) and Nile tilapia (Oreochromis niloticus) sampled in India. To gain a wider comparison, we included 11 whole genome sequences of Aeromonas spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 Aeromonas sequences examined had multiple AMR genes of which the Ambler classes B, C and D β-lactamase genes were the most dominant. The high similarity of AMR genes in the Aeromonas sequences obtained from different host species point to interspecies transmission of AMR genes. Our findings also show that all Aeromonas sequences examined encoded several multidrug efflux-pump proteins. As for genes linked to mobile genetic elements (MBE), only the class I integrase was detected from two fish isolates, while all transposases detected belonged to the insertion sequence (IS) family. Only seven of the 15 Aeromonas sequences examined had plasmids and none of the plasmids encoded AMR genes. In summary, our findings show that Aeromonas spp. isolated from different host species in India carry multiple AMR genes. Thus, we advocate that the control of AMR caused by Aeromonas spp. in India should be based on a One Health approach.

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Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling

Cited by 9 publications

References 31 publications

Key genes and potential drugs in age-related hearing loss: transcriptome analysis of cochlear hair cells in old mice

Key genes and potential drugs in age-related hearing loss: transcriptome analysis of cochlear hair cells in old mice

The mobile gene cassette carrying tetracycline resistance genes in Aeromonas veronii strain Ah5S-24 isolated from catfish pond sediments shows similarity with a cassette found in other environmental and foodborne bacteria

Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles

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