Identity of euchromatic bands from man to Cercopithecidae

Dutrillaux, B.; Viegas‐Péquignot, Evani; Couturier, Jérôme; Chauvier, G

doi:10.1007/bf00278726

Cited by 32 publications

(8 citation statements)

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“…(c) Pair number 4 with a paracentric inversion in one chromosome observed in a female C. nigrivittatus. the constitutive heterochromatin regions, as has previously been described in the species of the same genus: C. apella (Seuá nez et al, 1986;Freitas and Seuá nez, 1982;Matayoshi et al, 1987;Ponsà et al, 1995;García et al, 2003) and C. capucinus (Dutrillaux et al, 1978;Garcia et al, 1983;Carlà and Stanyon, 1992). In C. apella, previous reports have detected size variation of non-centromeric heterochromatin regions in chromosomes 4, 6, 11, 12, 17, 18 and 19 (Matayoshi et al, 1987;Ponsà et al, 1995).…”

Section: Resultssupporting

confidence: 57%

“…In Venezuela, C. nigrivittatus is widely distributed and represented by five subspecies (Hershkovitz, 1949;Cabrera, 1957;Bodini and Pé rezHerná ndez, 1987). Chromosome number (2n=52), R-banding and C-banding have been previously described (Dutrillaux et al, 1978;Martinez et al, 1999). Sequential G-/C-banding karyotypes, chromosome homologies among C. nigrivittatus, C. apella and human karyotypes, as well as evolutionary reorganisations that would explain these homologies, were published based upon G-banding comparison and ZOO-FISH results using all human chromosome probes (García et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, some primate species have been widely analysed, but in other published studies are scarce, as is the case of Cebus genus (Family Cebidae, Primates). In this group, C. apella (CAP) and C. capucinus (CCA) have been widely studied (Dutrillaux et al, 1978;Garcia et al, 1983;Matayoshi et al, 1987;Mudry, 1990;Ponsà et al, 1995;García et al, 2000García et al, , 2003, whereas no data on heterochromatin polymorphisms have been published for C. nigrivittatus or Cebus albifrons.…”

Section: Introductionmentioning

confidence: 99%

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New polymorphisms in a Cebus (Platyrrhini, Primates) species. The case of Cebus nigrivittatus

et al. 2004

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-Cytogenetic studies have been performed in six specimens of Cebus nigrivittatus with G-, and C-banding methods. Centromeric C+ bands have been detected in each chromosome pair, terminal C+ bands in chromosome pairs 1, 2, 3, 12/24 and 11, whereas interstitial C+ bands have been observed in chromosome pairs 4, 12/24, 8, 13, 16, 17, 18, 19, 20 and 21. The C-banding patterns show a degree of polymorphism as well as the presence of interstitial and terminal C-bands. A different morphology affecting the long arm of chromosome 4 due to a paracentric inversion is also reported.

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Section: Resultssupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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New polymorphisms in a Cebus (Platyrrhini, Primates) species. The case of Cebus nigrivittatus

et al. 2004

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“…The karyotypes of the genus Cebus are known to have large heterochromatic segments whose richness in G+C bases suggested by cytogenetic methods (21) was confirmed by using a biochemical analysis (22). Moreover, because a clear analogy exists between the euchromatic material of the chromosomes of Cebus and man (23,24), the comparison of the staining patterns produced by antibodies to Z-DNA in euchromatin may be considered a check of the stability of Z-DNA conformation during chromosome evolution.…”

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confidence: 96%

“…By in situ hybridization, it has been shown that these heterochromatic segments possess a very repetitive G+C-rich satellite DNA (22). These segments clearly differ from the juxtacentromeric heterochromatin, which is R-, T-, G-, and Q-band negative (21) and for which no information exists concerning G+C richness.…”

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confidence: 99%

Z-DNA immunoreactivity in fixed metaphase chromosomes of primates.

Viegas‐Péquignot¹,

Derbin²,

Malfoy³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Antibodies against Z-DNA bind to fixed metaphase chromosomes of man and Cebus albifrons (Platyrrhini, Primate). By indirect immunofluorescence and indirect immunoperoxidase techniques, a heavy staining is detected in some segments of chromosomes of C. albifrons. These segments correspond to Rband-positive heterochromatin, which has a high G+C-base content. Euchromatin of human and Cebus chromosomes show a weak and heterogeneous staining that consistently reproduces an R-and T-banding pattern in both species. Because chromosome homologies previously were demonstrated between these distantly related species by chromosome banding, our results suggest that Z-DNA has been conserved during the course of primate evolution.Repeating sequences of alternating dC and dG deoxynucleotides can form the left-handed duplex DNA conformation, named Z-DNA, as demonstrated by x-ray diffraction studies of crystals of (dC-dG)n (1-3) and fibers of poly (dG-dC)-poly (dG-dC) ( (23, 24), the comparison of the staining patterns produced by antibodies to Z-DNA in euchromatin may be considered a check of the stability of Z-DNA conformation during chromosome evolution.In this work we show (i) that anti-Z-DNA antibodies bind to metaphase chromosomes of man and Cebus in a distinct and specific way and (ii) that an undisputable correspondence exists between the antibody binding and the chromosome banding patterns. In euchromatin, a weak but distinct and consistent staining is observed in the segments corresponding to the Rand T-bands, which are thought to be richer in G-C base pairs both in man and Cebus. Slight differences of intensity are observed from band to band: they are the same for all of the chromosomal segments of man and Cebus, which were found homologous by banding pattern and by gene mapping comparison (23)(24)(25). This fact underlines the stability of Z-DNA conformation during the evolution of mammalian chromosomes. Furthermore, the additional heterochromatic segments existing in Cebus chromosomes exhibit a consistent and very strong binding to anti-Z-DNA antibodies.MATERIAL AND METHODS Obtention of Metaphase Chromosomes. Metaphase cells were obtained from tissue cultures after skin biopsy of a female of C. albifrons held in captivity at the Museum National d'Histoire Naturelle, Paris.Metaphase cells from human lymphocytes were cultured according to usual methods (26). Cell cultures were treated by colchicine for 1 or 2 hr and then for 10 min with a hypotonic solution of diluted human serum (1:6, vol/vol). Cells were fixed in most of the experiments for at least 40 min with two different solutions (solution I, ethanol/chloroform/acetic acid, 6:3:1, vol/ vol; solution II, ethanol/acetic acid, 3:1, vol/vol). A prolonged fixation (45 min to 48 hr), with only the last fixative solution was used for some human cultures.The following banding methods were used to characterize the chromosomes of the specimen of C. albifrons: R-banding 5890 The publication costs of this article were defrayed in part by page charge payment. T...

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Constitutive heterochromatin polymorphism in Lagothrix lagothricha cana, Cebus apella, and Cebus capucinus

Garcı́a

Miró

Estop

et al. 1983

American J Primatol

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We describe the C-bands in the karyotypes of Lagothrix lagothricha CURU, Cebus apella and Cebus capucinus. The C-banding patterns show both a high degree of polymorphism as well as the presence of terminal and interstitial C-bands. Varying amounts of heterochromatin result in dimorphism of some chromosome pairs. The high incidence of chromosome rearrangements found in the Cebidae may be due to the presence of terminal and interstitial C-bands.

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Identity of euchromatic bands from man to Cercopithecidae

Cited by 32 publications

References 9 publications

New polymorphisms in a Cebus (Platyrrhini, Primates) species. The case of Cebus nigrivittatus

New polymorphisms in a Cebus (Platyrrhini, Primates) species. The case of Cebus nigrivittatus

Z-DNA immunoreactivity in fixed metaphase chromosomes of primates.

Constitutive heterochromatin polymorphism in Lagothrix lagothricha cana, Cebus apella, and Cebus capucinus

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