Ideonella sakaiensis sp. nov., isolated from a microbial consortium that degrades poly(ethylene terephthalate)

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, rod-shaped bacterium, designed strain 201-F6 T , was isolated from a microbial consortium that degrades poly(ethylene terephthalate) (PET) collected in Sakai city, Japan, and was characterized on the basis of a polyphasic taxonomic study. The cells were motile with a polar flagellum. The strain contained cytochrome oxidase and catalase. It grew within the pH range 5.5-9.0 (optimally at pH 7-7.5) and at 15-42 ºC (optimally at 30-37 ºC). The major isoprenoid quinone was ubiquinone with eight isoprene units (Q-8). C 16 : 0 , C 17 : 0 cyclo, C 18 :1 !7c and C 12 : 0 2-OH were the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of genomic DNA was 70.4 mol%. Phylogenetic analysis using the 16S rRNA gene sequences showed that strain 201-F6 T was affiliated to the genus Ideonella, and was closely related to Ideonella dechloratans LMG 28178T (97.7 %) and Ideonella azotifigens JCM 15503 T (96.6 %). Strain 201-F6 T could be clearly distinguished from the related species of the genus Ideonella by its physiological and biochemical characteristics as well as by its phylogenetic position and DNA-DNA relatedness. Therefore, the strain represents a novel species of the genus Ideonella, for which the name Ideonella sakaiensis sp.

Biodegradation of waste PET

“…Further studies led to the discovery of a bacterial species that is able to metabolize amorphous PET without any assistance from other organisms in the consortium. This strain, a gram‐negative, aerobic, rod‐shaped bacterium, was found to be a new species of the genus Ideonella , and it was named Ideonella sakaiensis 201‐F6 .…”

“…While consortium No. 46 includes PET‐degrading I. sakaiensis 201‐F6 , other bacteria with specialized functions involved in PET degradation were isolated and characterized. Initially, Bacillus megaterium forms a biofilm on the PET film.…”

A Multi-OMIC Characterisation of Biodegradation and Microbial Community Succession Within the PET Plastisphere

“…Bacterial community structure was assessed via 16S rRNA gene sequencing obtained from the inoculum as well as from all ve treatments across six weeks of incubation (i.e. days 1,3,7,14,21,30,42), including separate analysis of planktonic and bio lm communities grown with the amorphous PET lms. Only two samples (replicate 2 from day 42 amorphous PET bio lm and replicate 1 from day 42 PET powder) as well as procedural controls (i.e.…”

“…One particularly noteworthy exception to this is in the degradation of polyethylene terephthalate (PET), where a number of PET hydrolases, termed PETases, have been identi ed [13]. The PETase that has garnered the most attention to date is that of the bacterium Ideonella sakaiensis, a terrestrial Betaproteobacterium isolated from outside a plastic bottle factory [14,15]. This PETase is different from other similar esterases as it exhibits higher hydrolytic activity on PET than other substrates and is active at lower temperatures.…”

Effect of mineral fertilizer encapsulated with zeolite and polyethylene terephthalate on the soil microbiota, pH and plant germination

“…Microorganisms known for their biodegradable ability to decompose different type of resistant compounds of hydrocarbon origin (Rusyn et al, 2003;Glaser et al, 2019;Ghatge et al, 2020) expand the possibilities of using compounds with long-term decomposition in the environment. The use of PET in the shell of the fertilizer in the minimum amount has prospects due to the isolation from the media exposed to PET, the bacteria Ideonella sakaiensis 201-F6, which are able to use PET as the main source of energy and are capable to biodegradation of environmentally stable polymer in 1.5-2 vegetation period (Tanasupawat et al, 2016;Danso et al, 2018;Liu et al, 2018).…”