IFCC method (1988) for tonometry of blood: Reference materials for pCO2 and pO2

Burnett, Robert W.; Covington, A. K.; Maas, Anton; Müller‐Plathe, O.; Weisberg, Herbert F.; Wimberley, P. D.; Zijlstra, W. G.; Siggaard-Anderson, O.

doi:10.1016/0009-8981(89)90141-1

Cited by 13 publications

(6 citation statements)

References 8 publications

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“…control of working conditions: the IFCC method was followed for tonometry of blood, particularly in terms of procedure and equilibrating conditions [6]. Improper handling during the anaerobic use of the Laue tonometer and gas humidification, for instance, yield inaccurate values [7].…”

Section: Discussionmentioning

confidence: 99%

Assessing the ABL 500 blood gas analyser

Gouget¹,

Andriamahatratra²,

Gourmelin³

et al. 1991

Journal of Analytical Methods in Chemistry

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The ABL 500 blood gas analyser from Radiometer has cordless electrodes and does not use a humidifier for calibrating gases. During the evaluation of the analytical performance of this instrument, the problem of p02 accuracy was approached by comparing the values obtained with two kinds of tonometry (film and bubble). An acceptable level of imprecision was demonstrated for all measured parameters. For within-run precision, with tonometry, coefficients of variation (CV) were ≆0.37% for pO2 and ≆0.52% for pCO2. A CV of 1.76% was found for day-to-day precision for both p02 and pCO2. In the linearity study, with both tonometry methods, and in the inter-instrument comparisons (the ABL was compared with the Ciba Corning 178), pO2 values obtained on the ABL 500 exhibited a slight overestimation above 150 mmHg (2.2-3.4% at 600 mmHg). This minor discrepancy is discussed with reference to the new design of the pO2 electrode, the algorithm for pO2 correction and the tonometry procedure. The results reported in this paper stress the importance of pO2 accuracy assessment for the evaluation of blood gas analysers.

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Section: Discussionmentioning

confidence: 99%

Assessing the ABL 500 blood gas analyser

Gouget¹,

Andriamahatratra²,

Gourmelin³

et al. 1991

Journal of Analytical Methods in Chemistry

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“…Samples collected in parallel were analyzed within 18 hours for P50 by multipoint tonometry. 11 Rats were then administered a lethal dose of halothane.…”

Section: Dose Determination Studymentioning

confidence: 99%

Effects of a Synthetic Allosteric Modifier of Hemoglobin Oxygen Affinity on Outcome From Global Cerebral Ischemia in the Rat

Grocott

Bart

Sheng

et al. 1998

Stroke

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Background and Purpose-Neuronal injury results from an insufficient supply of oxygen to the brain. This experiment examined whether a pharmacologically induced rightward shift of the partial pressure of oxygen at which 50% of hemoglobin is saturated (P50) would improve outcome from either incomplete and/or near-complete forebrain ischemia-induced hypoxia in the rat. Methods-For incomplete ischemia (attenuated electroencephalogram), fasted rats (nϭ17 to 19 per group) were given a synthetic allosteric modifier of hemoglobin affinity for oxygen (RSR13; 150 mg/kg IV) before or immediately after 20 minutes of bilateral carotid occlusion combined with a decrease in mean arterial pressure to 40 mm Hg. For near-complete ischemia (isoelectric electroencephalogram), rats (nϭ15 per group) were given RSR13 (150 mg/kg) at onset of reperfusion after 10 minutes of bilateral carotid occlusion combined with a decrease in mean arterial pressure to 30 mm Hg. In both experiments, control rats were given vehicle (0.9% NaCl IV) only. Outcome (defined as percent dead hippocampal CA1 neurons) was determined at 5 days after ischemia. Results-RSR13 (150 mg/kg) produced a 68% rightward shift of P50 (34Ϯ3 to 57Ϯ8 mm Hg). RSR13 reduced CA1 damage resulting from incomplete ischemia by 28% (Pϭ0.02), but only when administered at the onset of reperfusion. RSR13 had no effect on outcome from near-complete ischemia. Conclusions-A postischemic pharmacologically induced increase in P50 may improve outcome from incomplete global cerebral ischemia. More severe (near-complete) ischemia negates this benefit. (Stroke. 1998;29:1650-1655.)

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“…Equilibration of heparinized blood from healthy individuals was performed with the thin-film-tonometer IL 237® (Instrumentation Laboratory), following the IFCC recommendation (22). The following gas mixtures were used: For measurement of the cell count in the second group of experiments we used a Sysmex CC800® (Fa.…”

Section: Tonometrymentioning

confidence: 99%

Stability of pO 2 , pCO 2 and pH in Heparinized Whole Blood Samples: Influence of Storage Temperature with Regard to Leukocyte Count and Syringe Material

Schmidt

Müller‐Plathe²

1992

Clinical Chemistry and Laboratory Medicine

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Schmidt and M ller-Plathe: Stability of/?O 2 , pCO 2 and pH: Influence of storage temperature and blood cell metabolism 767 Eur. J. Clin. Chem. Clin. Biochem. Vol. 30, 1992, pp. 767-773 © 1992 Summary: Influences of storage temperature and blood cell metabolism in different types of syringes were investigated. Experiments were performed on blood samples with normal and elevated leukocyte counts. After equilibration with gas mixtures at normal pO 2 (86 mm Hg/11.5 kPa) and elevated pO 2 (140 mm Hg/18.7 kPa), sequential blood gas analyses were done within one hour. Storage temperatures were 4 °C or 22 °C.In the first group of experiments we compared glass samplers with plastic syringes at different storage temperatures with regard to deviations of blood gas concentrations. The analysed samples had a normal cell count. Blood stored in glass syringes in ice water served as the reference, and it displayed virtually no changes. The deviations of pCO 2 and pH were relatively small. In plastic syringes the greatest increases for pO 2 occurred after storage at 4 °C, which can be explained by the increased solubility of oxygen and the higher O 2 affinity of haemoglobin at 4 °C. When stored at room temperature, the deviations in plastic syringes were smaller.In a second group of experiments, the influence of cell metabolism was studied. Blood gases were analysed in samples with elevated leukocyte counts (20 χ 10 9

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IFCC method (1988) for tonometry of blood: Reference materials for pCO2 and pO2

Cited by 13 publications

References 8 publications

Assessing the ABL 500 blood gas analyser

Assessing the ABL 500 blood gas analyser

Effects of a Synthetic Allosteric Modifier of Hemoglobin Oxygen Affinity on Outcome From Global Cerebral Ischemia in the Rat

Stability of pO 2 , pCO 2 and pH in Heparinized Whole Blood Samples: Influence of Storage Temperature with Regard to Leukocyte Count and Syringe Material

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