IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma

Bergauer, Annika; Sopel, Nina; Kroß, Bettina; Vuorinen, Tytti; Xepapadaki, Paraskevi; Weiss, Scott T.; Blau, Ashley; Sharma, Himanshu; Kraus, Cornelia; Springel, Rebekka; Rauh, Manfred; Mittler, Susanne; Graser, Anna; Zimmermann, Theodor; Melichar, Volker O.; Krause, Alexander; Kowalski, Marek L.; Sobańska, Anna W.; Jartti, Tuomas; Lukkarinen, Heikki; Papadopoulos, Nikolaos G.; Finotto, Susetta

doi:10.1183/13993003.00969-2016

Cited by 32 publications

(35 citation statements)

References 30 publications

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“…The recruitment of the subjects, inclusion and exclusion criteria, as well as the time-scale for clinical visits and data collection, have been described recently [24][25][26], and patient characteristics are reported in Table 1. This study was performed on the WP1-cohort set up at the Department of Molecular Pneumology in Erlangen in collaboration with the local Children's Hospital.…”

Section: Human Studiesmentioning

confidence: 99%

“…The growth of RV1b and the description of the RV1b infection itself have been published previously in detail elsewhere [27]. PBMCs were then adjusted to a concentration of 1 3 10 6 viable cells/ml in complete culture medium, as described previously, and parts of them were challenged invitro or not with RV (RV1b) and were cultured for an additional 48 h to perform RNA gene array assays and further analyses.…”

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmcs) Frommentioning

confidence: 99%

“…The description of this method was recently published in detail elsewhere [27]. The description of this method was recently published in detail elsewhere [27].…”

Section: Rv Detection In Vivomentioning

confidence: 99%

“…PBMCs were then adjusted to a concentration of 1 3 10 6 viable cells/ml in complete culture medium, as described previously, and parts of them were challenged invitro or not with RV (RV1b) and were cultured for an additional 48 h to perform RNA gene array assays and further analyses. The growth of RV1b and the description of the RV1b infection itself have been published previously in detail elsewhere [27].…”

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmcs) Frommentioning

confidence: 99%

“…The detection of RV in nasopharyngeal swabs from the children was performed at the Department of Virology, University of Turku (Finland). The description of this method was recently published in detail elsewhere [27].…”

Section: Rv Detection In Vivomentioning

confidence: 99%

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Soluble ST2 regulation by rhinovirus and 25(OH)-vitamin D3 in the blood of asthmatic children

Haag

Sharma

Rauh

et al. 2018

Clinical and Experimental Immunology

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Paediatric asthma exacerbations are often caused by rhinovirus (RV). Moreover, 25(OH)-vitamin D3 (VitD3) deficiency during infancy was found associated with asthma. Here, we investigated the innate immune responses to RV and their possible modulation by 25(OH)-VitD3 serum levels in a preschool cohort of children with and without asthma. The innate lymphoid cell type 2 (ILC2)-associated marker, ST2, was found up-regulated in the blood cells of asthmatic children with low serum levels of 25(OH)-VitD3 in the absence of RV in their airways. Furthermore, in blood cells from control and asthmatic children with RV in their airways, soluble (s) ST2 (sST2) protein was found reduced. Asthmatic children with low 25(OH)-VitD3 in serum and with RV in vivo in their airways at the time of the analysis had the lowest sST2 protein levels in the peripheral blood compared to control children without RV and high levels of 25(OH)-VitD3. Amphiregulin (AREG), another ILC2-associated marker, was found induced in the control children with RV in their airways and low serum levels of 25(OH)-VitD3. In conclusion, the anti-inflammatory soluble form of ST2, also known as sST2, in serum correlated directly with interleukin (IL)-33 in the airways of asthmatic children. Furthermore, RV colonization in the airways and low serum levels of 25(OH)-VitD3 were found to be associated with down-regulation of sST2 in serum in paediatric asthma. These data indicate a counter-regulatory role of 25(OH)-VitD3 on RV-induced down-regulation of serum sST2 in paediatric asthma, which is relevant for the therapy of this disease.

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Section: Human Studiesmentioning

confidence: 99%

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmcs) Frommentioning

confidence: 99%

“…The description of this method was recently published in detail elsewhere [27]. The description of this method was recently published in detail elsewhere [27].…”

Section: Rv Detection In Vivomentioning

confidence: 99%

Section: Isolation Of Peripheral Blood Mononuclear Cells (Pbmcs) Frommentioning

confidence: 99%

Section: Rv Detection In Vivomentioning

confidence: 99%

See 3 more Smart Citations

Soluble ST2 regulation by rhinovirus and 25(OH)-vitamin D3 in the blood of asthmatic children

Haag

Sharma

Rauh

et al. 2018

Clinical and Experimental Immunology

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Respiratory infections regulated blood cells IFN‐β‐PD‐L1 pathway in pediatric asthma

Kölle

Haag

Vuorinen

et al. 2020

Immunity Inflam & Disease

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Background: Respiratory infections, in general, and rhinovirus infection specifically are the main reason for asthma exacerbation in children and programmed cell death protein 1 ligand (PD-L1) expression inhibits T cell responses. Objective: Could the interferon (IFN) type I expression in peripheral blood mononuclear cells (PBMCs) improve disease exacerbation in pediatric asthma? Results: Here we found increased level of PD-L1 messenger RNA (mRNA) in total blood cells isolated from preschool children with virus-induced asthma, with lower percentage of forced expiratory volume in 1 second and with high serum levels of the C-reactive-protein. Conclusions and Clinical Relevance: These data indicate that, in the presence of infection in the airways of preschool children, worse asthma is associated with induced PD-L1 mRNA expression in blood cells. Further, type I IFN, IFN-β, a cytokine that is involved in the clearance of infections, was found to be associated with a better lung function in asthmatic children. These data suggest that improving peripheral blood IFN type I expression in PBMCs in pediatric asthma could improve disease exacerbation due to suppressing PD-L1 expression in blood cells.

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Childhood asthma heterogeneity at the era of precision medicine: Modulating the immune response or the microbiota for the management of asthma attack

Lejeune

Deschildre

Rouzic

et al. 2020

Biochemical Pharmacology

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Exacerbations are a main characteristic of asthma. In childhood, the risk is increasing with severity. Exacerbations are a strong phenotypic marker, particularly of severe and therapy-resistant asthma. These earlylife events may influence the evolution and be involved in lung function decline.In children, asthma attacks are facilitated by exposure to allergens and pollutants, but are mainly triggered by microbial agents. Multiple studies have assessed immune responses to viruses, and to a lesser extend bacteria, during asthma exacerbation. Research has identified impairment of innate immune responses in children, related to altered pathogen recognition, interferon release, or anti-viral response. Influence of this host-microbiota dialog on the adaptive immune response may be crucial, leading to the development of biased T helper (Th)2 inflammation. These dynamic interactions may impact the presentations of asthma attacks, and have long-term consequences.The aim of this review is to synthesize studies exploring immune mechanisms impairment against viruses and bacteria promoting asthma attacks in children. The potential influence of the nature of infectious agents and/or preexisting microbiota on the development of exacerbation is also addressed. We then discuss our understanding of how these diverse host-microbiota interactions in children may account for the heterogeneity of endotypes and clinical presentations. Finally, improving the knowledge of the pathophysiological processes induced by infections has led to offer new opportunities for the development of preventive or curative therapeutics for acute asthma. A better definition of asthma endotypes associated with precision medicine might lead to substantial progress in the management of severe childhood asthma.

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IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma

Cited by 32 publications

References 30 publications

Soluble ST2 regulation by rhinovirus and 25(OH)-vitamin D3 in the blood of asthmatic children

Soluble ST2 regulation by rhinovirus and 25(OH)-vitamin D3 in the blood of asthmatic children

Respiratory infections regulated blood cells IFN‐β‐PD‐L1 pathway in pediatric asthma

Childhood asthma heterogeneity at the era of precision medicine: Modulating the immune response or the microbiota for the management of asthma attack

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