IgA Glycosylation in IgA Nephropathy

The Tn Antigen—Structural Simplicity and Biological Complexity

“…Glycoproteins containing core 1 or 2 O ‐glycans include the low‐density‐lipoprotein (LDL) receptor,136–138 the transferrin receptor,129, 139–141 glycophorin A,142–144 podoplanin,145–147 PSGL‐1,30, 148–150 CD34,151 endoglycan,152 zona pellucida glycoprotein ZP3,153, 154 CD43,155–157 CD45,158–161 the TRAIL receptor family,162 the neurotrophin receptor,163, 164 the MUC family of mucins (both membrane‐bound and secreted),165–169 platelet glycoprotein GPIbα,170 and platelet integrin GPIIb/IIIa (αIIbβ3) 171. Such core 1 and/or 2 O ‐glycans are also found in many secreted and serum glycoproteins, including human IgA1172–177 and IgD,178–180 erythropoietin,181–184 von Willebrand factor (vWF),185 blood coagulation factor X,186 serum apoC‐III,187–189 human chorionic gonadotropin (HCG),190–193 interleukin‐6 (IL‐6),194, 195 Tamm–Horsfall glycoprotein/uromodulin,196–199 Gc macrophage activating factor (GcMAF, a naturally derived form of human vitamin D binding protein),200 human urinary thrombomodulin,201 and human milk bile‐salt‐stimulated lipase 202–205. Furthermore, O ‐glycans are found in virtually all mucin secretions of epithelial cells and on the surface of all animal cells, including erythrocytes.…”

Determination of Aberrant O-Glycosylation in the IgA1 Hinge Region by Electron Capture Dissociation Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

“…Available techniques for analysis of IgA1 O-glycosylation, such as lectin binding assays, can identify the presence of Gal-deficient O-glycan chains (27)(28)(29)(30)(31). However, it is not known whether the Gal deficiency in IgAN patients occurs randomly or preferentially at specific sites.…”

Das Tn‐Antigen – strukturell einfach und biologisch komplex

“…[61,83,134] Das Tn-Antigen Angewandte Chemie die TRAIL-Rezeptor-Familie, [162] der Neurotrophinrezeptor, [163,164] die MUC-Familie der Mucine (Membran-gebundene und sezernierte), [165][166][167][168][169] Blutplättchen-Glycoprotein GPIba [170] und Blutplättchen-Integrin GPIIb/IIIa (aIIbb3). [171] Core-1-und/oder Core-2-O-Glycane kommen auch in vielen sezernierten und Serumglycoproteinen vor, einschließlich humanes Immunglobulin A1 (IgA1) [172][173][174][175][176][177] und D (IgD), [178][179][180] Erythropoetin, [181][182][183][184] von Willebrand-Faktor (vWF), [185] Blutgerinnungsfaktor X, [186] Serum ApoC-III, [187][188][189] humanes Choriongonadotropin (HCG), [190][191][192][193] Interleukin-6 (IL-6), [194,195] Tamm-Horsfall-Glycoprotein/Uromodulin, [196][197][198][199] Gc-Makrophagen aktivierender Faktor (GcMAF, eine natürlich entstehende Form des menschlichen Vitamin-D3 bindenden Proteins), [200] humanes Urin-Thrombomodulin …”