IgA subclass antibodies to gliadin in serum and intestinal juice of patients with coeliac disease

Volta, Umberto; Molinaro, N.; Fratangelo, D.; Bianchi, Francesco

doi:10.1111/j.1365-2249.1990.tb05232.x

Cited by 17 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the strong clonal relatedness between serum antibodies and gut PCs, we found anti-TG2 serum IgA to be mostly monomeric, whereas anti-TG2 antibodies secreted by gut PCs were J-chain-associated dimers and contained a larger fraction of the IgA2 subclass. Similar molecular differences have also been described for gluten-specific serum and gut IgA in celiac disease ( Volta et al., 1990 ). Together with the lack of correlation between gut PC numbers and serum antibody levels, these findings suggest that gut PCs are not the direct source of the specific serum IgA antibodies.…”

Section: Discussionsupporting

confidence: 74%

Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins

et al. 2017

View full text Add to dashboard Cite

SummaryMucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study the relationship between mucosal and systemic antibody responses, we took advantage of celiac disease patient samples for isolation of gut PCs as well as serum IgA and IgG reactive with a gluten-derived peptide or the autoantigen transglutaminase 2. Proteomic analysis of serum IgA revealed antigen-specific V-gene preferences, which matched those found in gut PCs. Further, gut PC CDR-H3 sequences were abundant in serum IgA but also detectable in serum IgG. Our data indicate that the same B cell clones that give rise to gut PCs also contribute to the serum antibody pool. However, serum IgA antibodies had a molecular composition distinct from that of IgA antibodies secreted in the gut, suggesting that individual B cell clones give rise to different PC populations.

show abstract

Section: Discussionsupporting

confidence: 74%

Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Inevitably, the proportions of these isoforms within the pool of TG2-specific IgA will affect the overall avidity of this pool. Although both dimeric and polymeric IgA have been shown to be present at low levels in the peripheral blood [29], the highest levels are present in the gut lumen -and thus produced by lamina propria plasma cells [30]. Because monomeric IgA is present predominantly in the serum, this could also explain the observed differences in TG2 IgA avidity, as observed locally by diNiro et al and systemically by us [25].…”

Section: Discussionsupporting

confidence: 54%

Serum autoantibodies directed against transglutaminase-2 have a low avidity compared with alloantibodies against gliadin in coeliac disease

Gelderman

Drop

Trouw

et al. 2014

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryCoeliac disease is characterized by intolerance to gliadin and related gluten components present in wheat, barley and rye. Coeliac disease patients harbour antibodies directed against alloantigens such as gliadin, but also against the autoantigen transglutaminase-2 (TG2). The type and quality of antibody responses provides insight into the underlying immune activation processes. Therefore, in this study we have analysed the avidity of the antibody response directed against the autoantigen TG2 and compared this with antibody responses against the alloantigens gliadin and Escherichia coli. We observed that the immunoglobulin (Ig)A autoantibody response directed against TG2 is of low avidity compared with the IgA response against the alloantigens gliadin and E. coli in the same patients; the same was true for IgG, both in IgA-deficient and in -sufficient coeliac patients. The observed avidities appear not to be related to disease stage, antibody levels, age or duration of exposure to gluten. In conclusion, in coeliac disease there is a clear difference in avidity of the antibody responses directed against the auto-and alloantigens, indicating different regulation or site of initiation of these responses.

show abstract

“…As far as IgA is concerned, most of the antibody present in serum is polymeric (17), mainly dimers without a secretory component and of the IgA l isotype (18)(19)(20); it has been suggested, on the basis of molecular size, that serum AGA IgA is mostly derived from the gut mucosa, but further work is needed on this point.…”

Section: Antibodymentioning

confidence: 99%

Anti‐gliadin Antibodies

Troncone,

Ferguson

1991

J. pediatr. gastroenterol. nutr.

View full text Add to dashboard Cite

IgA subclass antibodies to gliadin in serum and intestinal juice of patients with coeliac disease

Cited by 17 publications

References 14 publications

Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins

Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins

Serum autoantibodies directed against transglutaminase-2 have a low avidity compared with alloantibodies against gliadin in coeliac disease

Anti‐gliadin Antibodies

Contact Info

Product

Resources

About