2000
DOI: 10.1152/jappl.2000.89.4.1365
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IGF-I restores satellite cell proliferative potential in immobilized old skeletal muscle

Abstract: One of the key factors responsible for the age-associated reduction in muscle mass may be that satellite cell proliferation potential (number of doublings contained within each cell) could become rate limiting to old muscle regrowth. No studies have tested whether repeated cycles of atrophy-regrowth in aged animals deplete the remaining capacity of satellite cells to replicate or what measures can be taken to prevent this from happening. We hypothesized that there would be a pronounced loss of satellite cell p… Show more

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Cited by 227 publications
(74 citation statements)
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“…Thus, the FOXO family might act negatively in mammals as a downstream player in insulin or IGF signaling. IGF-1 stimulates the proliferation of skeletal muscle satellite cells (57). Mature skeletal muscle fibers are not able to proliferate.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, the FOXO family might act negatively in mammals as a downstream player in insulin or IGF signaling. IGF-1 stimulates the proliferation of skeletal muscle satellite cells (57). Mature skeletal muscle fibers are not able to proliferate.…”
Section: Discussionmentioning
confidence: 99%
“…Muscles from 5-6 animals from each of the two groups were pooled to provide sufficient tissue for satellite cell isolation; the methods for their isolation have been described in detail previously (9). These cells were cultivated on collagen-coated tissue culture plates using high serum conditions (Ham's F-10 nutrient mixture containing 20% fetal bovine serum, 1.0% chicken embryo extract, with 1% penicillin/streptomycin antibiotic mixture, and 1% L-glutamine) to minimize any loss of proliferative cells due to either apoptosis or terminal differentiation.…”
Section: Methodsmentioning
confidence: 99%
“…Determination of Cumulative Population Doublings-Cumulative number of population doublings attained over the course of the in vitro culture life span of WT and IGF-I Tg satellite cells was determined as described previously (9,22). At the time of cell isolation from the animal, cell populations from both groups were considered to be at 1 population doubling (PD).…”
Section: Methodsmentioning
confidence: 99%
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“…IGF-I was chosen as it has been shown to modulate the transcriptional activity and cell cycle control of other forkhead/winged helix transcription factors and influence the G 1 /S cell cycle progression (19). We hypothesized that if the cell cycle defect in the absence of Foxk1 was the result of impaired extrinsic cues, then exposure of Foxk1Ϫ/Ϫ myogenic progenitor cells to WT conditioned medium or IGF-I should rescue the cell cycle perturbation (i.e.…”
Section: Reduced Myogenic Progenitor Cell Population In the Absencementioning
confidence: 99%