IgG AND OTHER PROTEINS ASSOCIATED WITH HUMAN CARCINOMAS AND CANCER‐FREE TISSUE FROM THE SAME ORGANS

Abstract

Eluates of different tissues were prepared at 56°C using a continuous flow technique. More IgG and other serum proteins were found in liver tissue from patients with non‐malignant diseases than in liver tissue from healthy control individuals. Cancer‐free liver tissue from patients with carcinomas was similar to that of patients with non‐malignant diseases, but liver metastases contained twice the amount of IgG. No differences were found between non‐perfused carcinomas of the kidney and cancer‐free renal tissue, whereas more IgG was present in perfused carcinomas of the kidney than in cancer‐free renal tissue. Most eluates of the malignant tissues and of the cancer‐free renal tissue showed a reduced ratio of albumin to IgG compared to that of serum or extracts, indicating a binding of IgG to antigens and/or receptors.

TISSUE REACTIVITY OF IgG ELUTED FROM HUMAN CARCINOMAS

“…The IgG eluted from 8 tumours did not bind to the autologous tissue. The IgG eluted from 5 of these tumours was considered non-specifically bound, since the ratio of albumin to IgG was similar using extracts and eluates from these tumours ( 14,17). The other 3 tumours displayed a reduced ratio of albumin to IgG in the eluates compared to the corresponding extract (14,17).…”

“…We have previously shown (16,17) that eluates of human carcinomas contain considerable amounts of Ig. Parts of the IgG seem to be non-specifically bound to the tissue.…”