IgM and avidity of IgG antibodies in primary HHV-6 infections

Salonen, Matteus; Vaheri, Antti; Koskiniemi, Marjaleena

doi:10.1080/00365540701747119

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…Second, although the specificity of ELISA IgG determination is 98.8% and its sensitivity is 99.8% (28), data on the specificity and sensitivity of tests for the HHV-6 IgM antibody are contradictory. Parker and Weber (29) reported 98% IgM specificity, but Salonen et al (30) reported 77% sensitivity and 41% specificity. The manufacturer (Panbio) indicates that the kit used in the present study has a 52.5% serological sensitivity and a 87.8% clinical specificity.…”

Section: Discussionmentioning

confidence: 99%

Human Herpesvirus 6 Infection in Hemodialysis and Peritoneal Dialysis Patients

Altay¹,

Akay

Unverdi³

et al. 2011

Perit Dial Int

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Infection with HHV-6 is not rare in PD and HD patients. In addition, HHV-6 IgG seropositivity was significantly higher in HD patients than in control subjects and approached significance when compared with seropositivity in PD patients. Moreover, in HD patients, HHV-6 IgG seropositivity correlated with duration on HD. These preliminary findings provide insight into the pre-transplantation period for patients and may aid our understanding of how to best protect patients against HHV-6 after transplantation.

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Section: Discussionmentioning

confidence: 99%

Human Herpesvirus 6 Infection in Hemodialysis and Peritoneal Dialysis Patients

Altay¹,

Akay

Unverdi³

et al. 2011

Perit Dial Int

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show abstract

“…IgG is identified as being of low avidity if there is a reduction in the titre in the specimen analyzed in the assay carried out in denaturing conditions compared with the normal procedure, which indicating primary infection. Methods have been developed to characterize the avidity of IgG against measles [27], rubella [28,29], PVHB19 [30], HHV-6 [31]; EBV [32]; CMV [33], Dengue virus [34,35] and VZV and HSV [29].…”

Section: Indirect Diagnosis: Serologymentioning

confidence: 99%

“…IgG avidity assays are applicable to the diagnosis of primary infection, and permit differentiation between primary and secondary infection in herpes infections, especially those produced by CMV and VZV infections [29], since both diseases may produce specific IgM. In addition, they permit the confirmation of EBV infection [32] by detection of IgM anti-CMV, and are also useful to confirm or exclude primary rubella virus infection in pregnancy by detection of specific IgM [28,36].…”

Section: Indirect Diagnosis: Serologymentioning

confidence: 99%

Role of the Laboratory in the Diagnosis of Viral Exanthems~!2009-11-20~!2009-11-25~!2010-02-16~!

Ory¹,

Mosquera²,

Echevarrı́a³

2010

TOVACJ

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An important number of viruses (measles, rubella, human parvovirus B19, herpes simplex, varicella zoster, enteroviruses, dengue and others) can cause exanthematic diseases. Laboratory diagnosis of exanthems due to viral infections include the identification of the disease-causing virus (isolation, antigen detection or nucleic acid detection) and the determination of a specific serological response (seroconversion or specific IgM). The aim of virus isolation is the recovering of infectious viruses, being useful for molecular epidemiological studies. It is of application for measles, rubella, enterovirus, herpes simplex and varicella-zoster. However, due to its methodological complications, it is not of application in most laboratories. For some viruses, isolation is not practical, because there is no cell substrate available (Epstein-Barr, parvovirus B19). An alternative to viral isolation is the detection of antigens on clinical specimens (dengue). Thirdly, nucleic acid detection is a very useful approach for diagnosing viral exanthems, specially when multiplex assays recognising measles, rubella and parvovirus B19, or enterovirus and herpesvirus, are used. The best tool for the serological diagnosis is the detection of IgM, since it provides a rapid diagnosis during the first days of the disease. However it has some drawbacks when used to diagnose viral rashes (the presence of rheumatoid factor and specific IgG; the multiple reactivity due to polyclonal stimulation of memory lymphocytes; the cross-reactivity between viruses causing a similar clinical picture; the IgM synthesis in herpesvirus reactivations). In such cases, confirmatory serological approaches (establishment of seroprofiles, or avidity assays) are necessary. Both direct and indirect methods must be considered as complementary and the results obtained for each one should be evaluated in the context of the clinical profile and epidemiological history.

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IgM and avidity of IgG antibodies in primary HHV-6 infections

Cited by 2 publications

References 16 publications

Human Herpesvirus 6 Infection in Hemodialysis and Peritoneal Dialysis Patients

Human Herpesvirus 6 Infection in Hemodialysis and Peritoneal Dialysis Patients

Role of the Laboratory in the Diagnosis of Viral Exanthems~!2009-11-20~!2009-11-25~!2010-02-16~!

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