Ikappa Balpha -independent downregulation of NF-kappa B activity by glucocorticoid receptor

Heck, Stefanie; Bender, Klaus; Kullmann, Michael; Göttlicher, Martin; Herrlich, Peter; Cato, Andrew C. B.

doi:10.1093/emboj/16.15.4698

Cited by 278 publications

(158 citation statements)

References 64 publications

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“…For example, induction of glutamine synthase by GR-LS7 was very low (Figure 1), as described previously , but induction of IkBa by GR-LS7 (Table 1,`MAD-3'), as previously shown by Northern blotting (Heck et al, 1997) was comparable to the degree of induction seen in GR-wt. Of potential importance for this discrepancy, Scheinman et al (1995) reported no functional GREs in the MAD-3 (IkBa) promoter.…”

Section: Resultssupporting

confidence: 84%

Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis

et al. 2001

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To arrive at a better understanding of the e ects of the glucocorticoid component of chemotherap#y protocols on lymphocytic leukemia cells, we analysed early responses of T-lymphocytic leukemia cell lines Jurkat and CEM-C7, both of which undergo apoptosis in response to dexamethasone, via gene chips. Among genes identi®ed as repressed, a notable cluster seemed to be of importance for the processes of transcription, mRNA splicing and protein synthesis. Consequently, we assessed time-resolved uptake of uridine and methionine to monitor RNA and protein synthesis, along with parameters quantifying apoptosis. Repression of uptake to about 65% of that in untreated cells preceded the ®rst sign of apoptosis by several hours in both cell lines. In addition to this general repression of RNA and protein synthesis, several genes were found to be regulated that may contribute to synergistic action of glucocorticoids with other components of frequently used chemotherapy protocols such as antimetabolites, methotrexate and alkylating agents. Oncogene (2001) 20, 4324 ± 4336.

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Section: Resultssupporting

confidence: 84%

Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis

et al. 2001

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“…Repression occurred independently of the GR dimerization and DNA binding function and did not involve IkB synthesis in these cells . It thus seems that glucocorticoids do not induce IkB in all cell types and the induction of IkB is not required for transrepression of NF-kB (see also Heck et al, 1997;Dumont et al, 1998).…”

Section: Tethering Mechanismsmentioning

confidence: 99%

Cross-talk between glucocorticoid receptor and AP-1

2001

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“…Poly(A) + RNA preparation and Northern blots were carried out essentially as described by Heck et al (1997).…”

Section: Rna Preparation and Northern Blot Analysismentioning

confidence: 99%

Rapid signalling by androgen receptor in prostate cancer cells

Peterziel¹,

Mink²,

Schonert³

et al. 1999

Oncogene

236

157

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Androgens are important growth regulators in prostate cancer. Their known mode of action in target cells requires binding to a cytoplasmic androgen receptor followed by a nuclear translocation event and modulation of the expression of speci®c genes. Here, we report another mode of action of this receptor. Treatment of androgen responsive prostate cancer cells with dihydrotestosterone leads to a rapid and reversible activation of mitogen-activated protein kinases MAPKs (also called extracellular signal-regulated kinases or Erks). Transient transfection assays demonstrated that the androgen receptor-mediated activation of MAP kinase results in enhanced activity of the transcription factor Elk-1. This action of the androgen receptor di ers from its known transcriptional activity since it is rapid and insensitive to androgen antagonists such as hydroxy¯utamide or casodex. Biochemical studies as well as analyses with dominant negative mutants showed the involvement of kinases such as MAPK/Erk kinase, phosphatidyl-inositol 3-kinase and protein kinase C in the androgen receptormediated activation of MAP kinase. These results demonstrate a novel regulatory action of the androgen receptor and prove that in addition to its known transcriptional e ects, it also uses non-conventional means to modulate several cellular signalling processes.

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Ikappa Balpha -independent downregulation of NF-kappa B activity by glucocorticoid receptor

Cited by 278 publications

References 64 publications

Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis

Expression profiling of glucocorticoid-treated T-ALL cell lines: rapid repression of multiple genes involved in RNA-, protein- and nucleotide synthesis

Cross-talk between glucocorticoid receptor and AP-1

Rapid signalling by androgen receptor in prostate cancer cells

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