IL-17 Expression in Dermatitis Herpetiformis and Bullous Pemphigoid

Abstract

Dermatitis herpetiformis (DH) and bullous pemphigoid (BP) are skin diseases associated with eosinophilic and neutrophilic infiltrations. Although cytokines are critical for the inflammatory process, there are single findings concerning concentration of IL-17 in bullous diseases. The goal of this study was to assess IL-17 expression in DH and BP patients. Skin biopsies were taken from 10 DH, 14 BP patients and from 10 healthy subjects. The localization and expression of IL-17 was studied by immunohistochemistry and the serum concentration was measured by immunoassays. Expression of IL-17 in the epidermis and in influxed cells in dermis was detected in skin biopsies. Expression of IL-17 was statistically higher in epidermis and infiltration cells in specimens from BP than from DH patients. Examined interleukin expression was detected in perilesional skin of all patients but it was much lower than in lesional skin. The expression of IL-17 was not observed in biopsies from healthy people. Serum level of IL-17 was statistically higher in BP and DH groups as compared to control group. Our results provide the evidence that IL-17 may play an essential role in activating and recruiting eosinophils and neutrophils, which ultimately contribute to the tissue damage in DH and BP.

Analysis of serum cytokine levels in patients with pemphigus vulgaris

“…Th17'den salınan IL-17A; multipl proinflamatuvar mediatörleri etkileyerek etkisini gösteren bir sitokindir 10 . Th17 hücre aracılı inflamasyonla ilişkili olarak otoimmün büllöz hastalıklar arasından büllöz pemfigoid ile ilişkisinin daha güçlü olduğuna dair kanıtlar mevcuttur 23,24 . Yaptığımız literatür taramalarında Th17 hücrelerden salınan IL-17A'nın PV ile ilişkisini araştıran çok fazla çalışmaya rastlamadık.…”

From bench to bedside: evolving therapeutic targets in autoimmune blistering disease

“…Generated from Th17 cells, subset of T helper cells, IL‐17, induces expression of cytokines and chemokines (IL‐1β, GM‐CSF, G‐CSF, CXCL1, CXCL2) and MMP‐9 that facilitate granulocyte recruitment, activation and ultimately protease release that results in characteristic BMZ degradation . IL‐17 is elevated in BP patients’ serum and blister fluid and has been implicated in mediating tissue destruction . Recent experimental studies have identified that mouse models deficient in IL‐17a do not develop BP .…”

“…[158][159][160][161][162][163] IL-17 is elevated in BP patients' serum and blister fluid and has been implicated in mediating tissue destruction. [164][165][166] Recent experimental studies have identified that mouse models deficient in IL-17a do not develop BP. 167 Additionally, mice treatment with anti-IL-17a antibodies exhibited significantly reduced disease development.…”

BP180- and BP230-specific IgG autoantibodies in pruritic disorders of the elderly: a preclinical stage of bullous pemphigoid?

“…Its extracellular noncollagenous 16A (NC16A) domain harbours the major T-cell and B-cell epitopes. 63,64 Additional immune modulatory T-cell subsets, such as Treg cells and cd T cells, are significantly reduced in BP based on preliminary studies. 53,54 Most of the autoreactive, BP180-specific T cells exhibit a Th2 profile, 53,55 whereas BP180-specific peripheral T cells from healthy individuals are dominated by the Th1 type.…”

“…61,62 Elevated numbers of Th17 cells and IL-17A levels were observed in the skin lesions of patients with BP, and a potential involvement in BP was therefore assumed. 63,64 Additional immune modulatory T-cell subsets, such as Treg cells and cd T cells, are significantly reduced in BP based on preliminary studies. [65][66][67] While the BP180-NC16A domain represents the immunodominant region for T cells, it is also targeted by IgG autoantibodies from patients with BP.…”