IL-5 Levels in Nasosorption and Sputosorption Correlate with Sputum Eosinophilia in Allergic Asthma

Melo, José Tavares de; Tunstall, Tanushree; Pizzichini, Márcia Margaret Menezes; Silva, Rosemeri Maurici da; Rocha, Cristiane Cinara; Dal‐Pizzol, Felipe; Gonçalves, Jussara Maria; Hansel, Trevor T.; Thwaites, Ryan S.; Pizzichini, Emílio

doi:10.1164/rccm.201807-1279le

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…The biomarkers of type-2-high asthma are believed to develop the pathological progress of asthma. IL-4 promotes the synthesis of Ig E from B cells, and IL-13 induces goblet cell metaplasia associated with mucus hypersecretion, and IL-5 accelerates influx of eosinophils into airways (Grunig et al, 1998;Xia et al, 2018;Busse et al, 2019;Melo et al, 2019). In addition, IL-17 can drive goblet cell metaplasia and mucus hypersecretion in primary human airway epithelia (Pezzulo et al, 2019).Our results suggested that FMT treatments suppressed OVA-induced inflammatory biomarkers expression of IL-4, IL-5, IL-13, IL-17A, and Ig E, and thereby contributed to the reduction of influx of leukocytes and goblet cell metaplasia evidenced by lung histopathology.…”

Section: Discussionmentioning

confidence: 99%

Formononetin Attenuates Airway Inﬂammation and Oxidative Stress in Murine Allergic Asthma

et al. 2020

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Allergic asthma has been considered as a respiratory disorder with pathological features of airway inflammation and remodeling, which involves oxidative stress. Formononetin (FMT) is a bioactive isoflavone obtained from Chinese herb Radix Astragali, and has been reported to have notable anti-inflammatory and antioxidant effects in several diseases. The purpose of our study was to elaborate the effects of FMT on asthma and the underlying mechanisms. To establish allergic asthma model, BALB/c mice were given ovalbumin (OVA) sensitization and challenge, treated with FMT (10, 20, 40 mg/kg) or dexamethasone (2 mg/kg). The effects of FMT on lung inflammation and oxidative stress were assessed. In OVA-induced asthmatic mice, FMT treatments significantly ameliorated lung function, alleviated lung inflammation including infiltration of inflammatory cells, the elevated levels of interleukin (IL)-4, IL-5, and IL-13, immunoglobulin (Ig) E, CC motif chemokine ligand 5 (CCL5, also known as RANTES), CCL11 (also called Eotaxin-1), and IL-17A. In addition, FMT treatments eminently blunted goblet cell hyperplasia and collagen deposition, and remarkably reduced oxidative stress as displayed by decreased reactive oxygen species (ROS), and increased superoxide diamutase (SOD) activity. Furthermore, to clarify the potential mechanisms responsible for the effects, we determined the inflammation and oxidation-related signaling pathway including nuclear factor kappa b (NF-kB), c-Jun N-terminal kinase (JNK), and the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). FMT treatments appeared to dramatically inhibit the activation of NF-kB and JNK, significantly elevated the expression of heme oxygenase 1 (HO-1) but failed to activate expression of Nrf2. In conclusion, our study suggested that FMT had the therapeutic effects in attenuating airway inflammation and oxidative stress in asthma.

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Section: Discussionmentioning

confidence: 99%

Formononetin Attenuates Airway Inﬂammation and Oxidative Stress in Murine Allergic Asthma

et al. 2020

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“…Moreover, nasal CCL‐26 is significantly correlated with sputum CCL‐26 (and IL‐13 and IL‐5) levels, which in turn is linked tightly with airway eosinophilia 17 . Minimally invasive sampling using nasosorption ® and sputosorption ® has offered some promise in providing surrogate markers of eosinophilic asthma, though these techniques require further study 43 . They are based on adsorptive materials, which allow efficient recovery of analytes (Table 1).…”

Section: Noninvasive and Minimally Invasive Diagnostics Of Allergic Amentioning

confidence: 99%

Noninvasive and minimally invasive techniques for the diagnosis and management of allergic diseases

Baumann

Untersmayr

Zissler

et al. 2021

Allergy

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Allergic diseases of the (upper and lower) airways, the skin and the gastrointestinal tract, are on the rise, resulting in impaired quality of life, decreased productivity, and increased healthcare costs. As allergic diseases are mostly tissue‐specific, local sampling methods for respective biomarkers offer the potential for increased sensitivity and specificity. Additionally, local sampling using noninvasive or minimally invasive methods can be cost‐effective and well tolerated, which may even be suitable for primary or home care sampling. Non‐ or minimally invasive local sampling and diagnostics may enable a more thorough endotyping, may help to avoid under‐ or overdiagnosis, and may provide the possibility to approach precision prevention, due to early diagnosis of these local diseases even before they get systemically manifested and detectable. At the same time, dried blood samples may help to facilitate minimal‐invasive primary or home care sampling for classical systemic diagnostic approaches. This EAACI position paper contains a thorough review of the various technologies in allergy diagnosis available on the market, which analytes or biomarkers are employed, and which samples or matrices can be used. Based on this assessment, EAACI position is to drive these developments to efficiently identify allergy and possibly later also viral epidemics and take advantage of comprehensive knowledge to initiate preventions and treatments.

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“…An example of ongoing research into non-invasive alternatives to sputum induction and more feasible measurements of airway biomarkers are through the recently developed absorptive nasal strip technology. Technology within absorptive strips permits the sampling of mucosal fluid within the upper respiratory tract109 and measurement of airway inflammatory biomarkers, successfully shown to reflect sputum eosinophilia in a recent small study in asthma 110. As a result, the powerful use of new technologies that convert ongoing advances of biomedical research into user-friendly tools that supplements and enhances current clinical tools can facilitate inter-professional communication between basic scientists, medical researchers and clinicians 111…”

Section: Strategies Related To Organizational Factorsmentioning

confidence: 99%

<p>Approaches to the assessment of severe asthma: barriers and strategies</p>

Majellano¹,

Clark²,

Winter³

et al. 2019

JAA

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Asthma is a chronic condition with great variability. It is characterized by intermittent episodes of wheeze, cough, chest tightness, dyspnea and backed by variable airflow limitation, airway inflammation and airway hyper-responsiveness. Asthma severity varies uniquely between individuals and may change over time. Stratification of asthma severity is an integral part of asthma management linking appropriate treatment to establish asthma control. Precision assessment of severe asthma is crucial for monitoring the health of people with this disease. The literature suggests multiple factors that impede the assessment of severe asthma, these can be grouped into health care professional, patient and organizational related barriers. These barriers do not exist in isolation but interact and influence one another. Recognition of these barriers is necessary to promote precision in the assessment and management of severe asthma in the era of targeted therapy. In this review, we discuss the current knowledge of the barriers that impede assessment in severe asthma and recommend potential strategies for overcoming these barriers. We highlight the relevance of multidimensional assessment as an ideal approach to the assessment and management of severe asthma.

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IL-5 Levels in Nasosorption and Sputosorption Correlate with Sputum Eosinophilia in Allergic Asthma

Abstract: Smoking duration alone provides stronger risk estimates of chronic obstructive pulmonary disease than pack-years.

Cited by 11 publications

References 10 publications

Formononetin Attenuates Airway Inﬂammation and Oxidative Stress in Murine Allergic Asthma

Formononetin Attenuates Airway Inﬂammation and Oxidative Stress in Murine Allergic Asthma

Noninvasive and minimally invasive techniques for the diagnosis and management of allergic diseases

<p>Approaches to the assessment of severe asthma: barriers and strategies</p>

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