IL28B rs12979860 Gene Polymorphism in Egyptian Patients with Chronic Liver Disease Infected with HCV

Abstract

Background: Egypt has one of the highest prevalences of hepatitis C virus (HCV) infection worldwide. Although the IL28B gene polymorphism has been shown to modify the course of chronic HCV infection, this has not been properly assessed in the Egyptian population. Materials and Methods: The IL28B rs12979860 single nucleotide polymorphism (SNP) was therefore examined in 256 HCV-infected Egyptian patients (group II) at different stages of disease progression and in 48 healthy volunteers (group I). Group II was subdivided into GII-A (chronic hepatitis patients, n=119), GII-B (post hepatitis cirrhosis, n=66) and GII-C (HCC on top of cirrhosis, n=71). Results: The C/T genotype was the commonest in all groups. It was more frequent in GI (52%) than in GII (48%). There was no significant difference in the frequency of C/T and C/C or T/T genotypes between groups and subgroups (p=0.82). Within the subgroups; the C/C genotype was more common in GII-B while C/T and T/T genotypes were more common in GII-C, though with no significant difference (p=0.59 and p=0.80). There was no significant association between IL28B rs12979860 SNP and viral load, ALT, AFP level, METAVIR scores for necro-inflammation and fibrosis, and Child-Pugh classification. Conclusions: 1) IL28Brs12979860 C/T genotype is the commonest genotype in HCV-associated CH and HCC in Egypt. 2) IL28Brs12979860 polymorphisms are not associated with disease progression or aggression (histological staging, severity of fibrosis in CH or the incidence of post-HCV HCC). 3) Differences in IL28Brs12979860 genotypes could be a consequence of environmental or ethnic variation.

Association between IL28B rs12979860 single nucleotide polymorphism and the frequency of colonic Treg in chronically HCV-infected patients

“…Overall, 67.5 % of the patients were genotype CT, 22.5 % were genotype CC, and 10 % were genotype TT. A previous study suggested that the frequencies of these IL28B genotypes in healthy controls in Egypt are CT (52.1 %), followed by CC (29.2 %) and TT (18.8 %), which are comparable to those observed in our study [40]. The NR genotyped group was distributed as 4 (20 %) CC, 13 (65 %) CT, and 3 (15 %) TT.…”

Data Mining and Machine Learning Algorithms Using <i>IL28B</i> Genotype and Biochemical Markers Best Predicted Advanced Liver Fibrosis in Chronic Hepatitis C

“…The results of this study revealed IL28B rs12979860 C T genotype as the commonest genotype (68.9 ) and is in agreement with previous studies done on Egyptian patients (19,(30)(31)(32), suggesting a possible role of this genotype in the transition of a normal hepatocyte into an abnormal one. This genotype distribution was suggested to be a consequence of environmental or ethnic variation (30,32).…”

“…FIB-4 was first described in HCV HIV co-infection, and later validated as an accurate marker for the diagnosis of fibrosis stages in patients with HCV mono-infection (17,40), particularly advanced fibrosis and cirrhosis. At different cut-off values the accuracy of FIB-4 approached 70 (41) with high sensitivity and moderate specificity (32). For predicting significant fibrosis, FIB-4 had an AUC value of 0.645, as reported by Alboraie et al (17).…”

“…This genotype distribution was suggested to be a consequence of environmental or ethnic variation (30,32).…”

Host genetic factors associated with hepatocellular carcinoma in patients with hepatitis C virus infection: A systematic review

“…However, this is not unexpected due to different patterns of correlation between SNPs in patients from different ethnic origin. For rs12979860, two studies found a significant association with HCC and two did not . Additionally, one study found a significant association with cirrhosis, although most studies using cirrhosis or fibrosis as an outcome did not find an association.…”