Illumina Sequencing Reveals the First Near-Complete Genome Sequence of
            <i>Ugandan Passiflora Virus</i>

Mbeyagala, E. K.; Maina, Solomon; Macharia, Mercy; Mukasa, Settumba B.; Holton, Timothy A

doi:10.1128/mra.00358-19

Cited by 4 publications

(2 citation statements)

References 12 publications

(13 reference statements)

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“…Plant virus diagnostic techniques have evolved with greater adoption of HTS, which involve sequencing of the total nucleic acid followed by bioinformatics (Adams et al, 2009;Maree et al, 2018). HTS has proven to be reliable and highly sensitive for virus detection and discovery (Al Rwahnih et al, 2009;Maina et al, 2016c;Maina et al, 2016d;Maina et al, 2016e;Maina et al, 2017d;Maina et al, 2017e;Maina and Jones, 2017g;Maina et al, 2019b), and when combined with biological information, has successfully resolved many disease complexes in agricultural and horticultural crops around the world (Maree et al, 2018;Wamaitha et al, 2018;Mbeyagala et al, 2019). In plant health diagnostics, the gold standards utilized by plant quarantine programs globally are biological indexing using indicator plants, serology, PCR, and electron microscopy.…”

Section: Overview Of High-throughput Sequencing Approaches In Plant V...mentioning

confidence: 99%

High-throughput sequencing for plant virology diagnostics and its potential in plant health certification

Maina,

Donovan,

Plett

et al. 2024

Front. Hortic.

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High-throughput sequencing (HTS) technologies have revolutionized plant virology through simultaneous detection of mixed viral infections. HTS advances have uncovered and improved understanding of virus biology, ecology, and evolution which is vital for viral disease management. Plant viruses continue to threaten global agricultural productivity and strict quarantine measures are essential to prevent the introduction and spread of virulent viruses around the world. The gradual decrease in HTS operational costs, including improved computational systems and automation through robotics, has facilitated the adoption of this tool for plant diagnostics, including its use in surveillance and quarantine programs. However, the speed of technology advancements and distinct HTS chemistries, laboratory procedures, data management, and bioinformatic analyses have proven challenging. In addition, the lack of viral species reference sequences, compared with the estimated number of distinct viral taxa, makes classification and identification of novel viruses difficult. There is a need for standardized HTS testing, especially within plant health programs. In this review, we consider the application of HTS in plant virology, explore the technical challenges faced and the opportunities for HTS in plant health certification. We propose standards for overcoming current barriers and for ensuring reliable and reproducible results. These efforts will impact global plant health by reducing the risk of introduction and the spread of damaging novel viruses.

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Section: Overview Of High-throughput Sequencing Approaches In Plant V...mentioning

confidence: 99%

High-throughput sequencing for plant virology diagnostics and its potential in plant health certification

Maina,

Donovan,

Plett

et al. 2024

Front. Hortic.

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“…Later studies, however, found that the causal agent of PWD in Brazil and South Africa is cowpea aphid-borne mosaic virus (CABMV), while that in Japan is East Asian Passiflora virus (EAPV) (Iwai et al 2006). More recently, many other viruses were reported to cause PWD, some of which, like Ugandan passiflora virus, have been shown to be a limiting factor for passionfruit production locally (Ochwo-Ssemakula et al 2011;Mbeyagala et al 2019). Despite their diversity in species and differences in geographical distribution, all PWD-causing viruses are positive-sense RNA viruses belonging to the genus Potyvirus under the family Potyviridae (Lefkowitz et al 2018).…”

Section: Introductionmentioning

confidence: 99%

A clade of telosma mosaic virus from Thailand is undergoing geographical expansion and genetic differentiation in passionfruit of Vietnam and China

Lian

Lin

et al. 2021

Phytopathol Res

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Passionfruit (Passiflora edulis) is widely cultivated in tropical and subtropical regions around the world. Several viruses of the genus Potyvirus pose serious threat to passion fruit production. The origin, dispersal and evolution of these potyviruses, however, are poorly understood. Here, we investigated the genetic structure of telosma mosaic virus (TelMV), a potyvirus that infects passionfruit in East and Southeast Asia, after a survey of its incidence in passionfruit plants of China. The phylogeny inferred from 140 nucleotide sequences of the coat protein (CP) gene of TelMV, including 96 determined in this study, separated this virus into 4 clades. TelMV isolates from passionfruit were placed into Clade 1–3, while those from other plant species into Clade 4. Interestingly, TelMV isolates of passionfruit from Thailand were found in all the three clades of Clade 1–3, but those from China and Vietnam were found exclusively in Clade 1. Nevertheless, TelMV isolates within Clade 1 tended to cluster according to their geographical origin. Geographical populations from Thailand, Taiwan and Hainan islands of China showed significant genetic differences with one another and with those from Guangxi, Fujian, Guangdong, Yunnan and Jiangsu provinces of China. Altogether, these data suggest that several distinct TelMV clades had arisen from the passionfruit of Thailand, but only one of which was dispersed. In expanding its distribution, this clade of TelMV has undergone geography-associated evolution. Further studies on this hypothesis may shed new insights into mechanisms underlying the emergence of potyviral diseases in passionfruit plants.

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Research Progress on Viruses of Passiflora edulis

Wu,

Ma,

Zhang

et al. 2024

Biology

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Passiflora edulis, also known as passion fruit, is celebrated for its rich nutritional content, distinctive flavour, and significant medicinal benefits. At present, viral diseases pose a major challenge to the passion fruit industry, affecting both the production and quality of the fruit. These diseases impede the sustainable and healthy growth of the passion fruit sector. In recent years, with the expansion of P. edulis cultivation areas, virus mutations, and advances in virus detection technology, an increasing number of virus species infecting P. edulis have been discovered. To date, more than 40 different virus species have been identified; however, there are different strains within the same virus. This poses a challenge for the control and prevention of P. edulis virus disease. Therefore, this review discusses the different types of viruses and their characteristics, modes of transmission, and effects on the growth of the passion fruit plant, as well as the mechanisms of virus generation and preventive measures, with the hope that these discussions will provide a comprehensive understanding of and countermeasures for viruses in passion fruit.

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Illumina Sequencing Reveals the First Near-Complete Genome Sequence of Ugandan Passiflora Virus

Abstract: Here, we present the first near-complete genome of Ugandan Passiflora virus (UPV) sequenced from a symptomatic sample of KH7 passion fruit (Passiflora edulis Sims) variety. UPV had limited amino acid identity with other potyviruses known to cause passion fruit woodiness disease (PWD).

Cited by 4 publications

References 12 publications

High-throughput sequencing for plant virology diagnostics and its potential in plant health certification

High-throughput sequencing for plant virology diagnostics and its potential in plant health certification

A clade of telosma mosaic virus from Thailand is undergoing geographical expansion and genetic differentiation in passionfruit of Vietnam and China

Research Progress on Viruses of Passiflora edulis

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