Illuminating the bacterial microbiome of Australian ticks with 16S and Rickettsia-specific next-generation sequencing

Greay, Telleasha L.; Evasco, Kimberly L.; Evans, Megan L.; Oskam, Charlotte L.; Magni, P.A.; Ryan, Una; Irwin, Peter

doi:10.1016/j.crpvbd.2021.100037

Cited by 11 publications

(10 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and Rickettsia sp. endosymbionts comprising a MRA of 31.27% and 13.73%, respectively, in H. bancrofti samples (Figures 3 , S4 , S6 ) (Beninati et al., 2009 ; Greay et al., 2021 ). No dominant bacterial endosymbiont was identified in the single I. trichosuri pool, although Mycobacteriaceae were highly abundant (MRA: 17.2%).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Metatranscriptomic profiling reveals diverse tick‐borne bacteria, protozoans and viruses in ticks and wildlife from Australia

Gofton

Blasdell

Taylor

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

Tick-borne zoonoses are emerging globally due to changes in climate and land use.While the zoonotic threats associated with ticks are well studied elsewhere, in Australia, the diversity of potentially zoonotic agents carried by ticks and their significance to human and animal health is not sufficiently understood. To this end, we used untargeted metatranscriptomics to audit the prokaryotic, eukaryotic and viral biomes of questing ticks and wildlife blood samples from two urban and rural sites in New South Wales, Australia. Ixodes holocyclus and Haemaphysalis bancrofti were the main tick species collected, and blood samples from Rattus rattus, Rattus fuscipes, Perameles nasuta and Trichosurus vulpecula were also collected and screened for tick-borne microorganisms using metatranscriptomics followed by conventional targeted PCR to identify important microbial taxa to the species level. Our analyses identified 32 unique tick-borne taxa, including 10 novel putative species. Overall, a wide range of tick-borne microorganisms were found in questing ticks including haemoprotozoa such as Babesia, Theileria, Hepatozoon and Trypanosoma spp., bacteria such as Borrelia, Rickettsia, Ehrlichia, Neoehrlichia and Anaplasma spp., and numerous viral taxa including Reoviridiae (including two coltiviruses) and a novel Flaviviridae-like jingmenvirus. Of note, a novel hard tick-borne relapsing fever Borrelia sp. was identified in questing H. bancrofti ticks which is closely related to, but distinct from, cervid-associated Borrelia spp. found throughout Asia. Notably, all tick-borne microorganisms were phylogenetically unique compared to their relatives found outside Australia, and no foreign tick-borne human pathogens such as Borrelia burgdorferi s.l. or Babesia microti were found. This work adds to the growing literature demonstrating that Australian ticks harbour a unique and endemic microbial fauna, including potentially zoonotic agents which should be further studied to determine their relative risk to human and animal health.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Conversely, P. nasuta samples formed a distinct cluster separate from other wildlife samples, indicating a unique microbial community profile (Figure 2). (Beninati et al, 2009;Greay et al, 2021).…”

Section: Metatranscriptomic Analysismentioning

confidence: 99%

Metatranscriptomic profiling reveals diverse tick‐borne bacteria, protozoans and viruses in ticks and wildlife from Australia

Gofton

Blasdell

Taylor

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

show abstract

“…This was clearly demonstrated via the results achieved when testing our nanopore sequencing method on VBB-positive controls ( Table 2 ). Here, closely related Rickettsia species were distinguishable via the use of the full-length 16S rRNA gene, a feat that would not have been achievable using one or a few hypervariable regions given the highly conserved nature of this gene across many pathogenic members of the genus ( 42 , 43 ). Furthermore, through our method, reads were obtainable from a range of different sample types, including from minute quantities, such as dots of culture on slides, with genomic DNA concentrations as low as 0.05 ng/μL still detectable for Anaplasma phagocytophilum and Ehrlichia chaffeensis .…”

Section: Discussionmentioning

confidence: 99%

Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma

et al. 2022

View full text Add to dashboard Cite

show abstract

“…This method is among the most accurate for diagnosing Rickettsia infection since, according to the data analysed in previous investigations using the real-time PCR method, its detection in the blood is problematic because it is a bacterium that multiplies within the endothelial cells [ 54 ]. Nevertheless, the future of confirmation is focused on genome sequencing and metagenomics [ 55 , 56 ]. Reports on the use of next-generation sequencing (NGS) and metagenomics to diagnose Rickettsia spp.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of zoonotic and non-zoonotic Rickettsia in horses: A systematic review and meta-analysis

Bonilla-Aldana

Castaño-Betancourt

Ortega-Martínez

et al. 2023

New Microbes and New Infections

View full text Add to dashboard Cite

Illuminating the bacterial microbiome of Australian ticks with 16S and Rickettsia-specific next-generation sequencing

Cited by 11 publications

References 145 publications

Metatranscriptomic profiling reveals diverse tick‐borne bacteria, protozoans and viruses in ticks and wildlife from Australia

Metatranscriptomic profiling reveals diverse tick‐borne bacteria, protozoans and viruses in ticks and wildlife from Australia

Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma

Prevalence of zoonotic and non-zoonotic Rickettsia in horses: A systematic review and meta-analysis

Contact Info

Product

Resources

About