Iloprost Induces Tertiary Dentin Formation

Limjeerajarus, Chalida Nakalekha; Chanarattanubol, Thichaporn; Trongkij, Panruethai; Rujiwanichkul, Mirantee; Pavasant, Prasit

doi:10.1016/j.joen.2014.07.002

Cited by 16 publications

(10 citation statements)

References 46 publications

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“…Local neovascularization around the site of injury during the early inflammatory phase is required for prevention of pulp tissue necrosis (29). In agreement with our previous findings (8,9), iloprost was able to increase HDPC proliferation and VEGF expression. The prolonged release of iloprost in vitro for up to 72 h might help to extend the healing of the dental pulp and further promote angiogenesis.…”

Section: Discussionsupporting

confidence: 91%

“…Iloprost, a PGI 2 analog, has been used in animal models and in patients with syndromes involving vascular deficiency in order to promote revascularization and increase blood flow (6,7). Previous studies have shown that iloprost increases the expression of VEGF and proliferative growth factors in human dental pulp cells in vitro, as well as increasing dental pulp blood flow and tertiary dentin formation in vivo (8,9). A recent study has also demonstrated that iloprost can increase the expression of VEGF in periodontal ligament cells, which are another type of mesenchymal stem cell that could be employed to achieve dentin and hard tissue formation as well as tooth revascularization (10).…”

Section: Originalmentioning

confidence: 99%

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Prolonged release of iloprost enhances pulpal blood flow and dentin bridge formation in a rat model of mechanical tooth pulp exposure

et al. 2019

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The aim of this study was to investigate the effect of prolonged release of iloprost, a prostacyclin analog, on angiogenesis and dental pulp healing in a rat model of mechanical pulp exposure. The profile of iloprost release from poly (lactic-co-glycolic) acid (PLGA) microspheres was evaluated, and expression of vascular endothelial growth factor (VEGF) mRNA was determined. The molars of rats were subjected to mechanical pulp exposure and 5 different forms of treatment: Ca(OH) 2 , PLGA (blank), iloprost, and iloprost/PLGA. Blood flow was determined at 0, 3, and 7 days using laser Doppler flowmetry. After 30 days, the tooth specimens were collected, and subjected to micro-CT and immunohistological analysis. The results showed that iloprost release from the microspheres was prolonged for 4 days, and that the treatment increased tooth blood flow for up to 7 days. At 30 days, an increase of mineralized tissue formation and dentin bridge formation was observed in the iloprost and iloprost/PLGA microsphere groups. VEGF expression was significantly increased in the iloprost/PLGA microsphere group relative to the other groups. In conclusion, this PLGA microsphere iloprost delivery system significantly increased dental pulp blood flow in a prolonged manner and increased tertiary dentin formation in this rat pulp injury model. Prolonged prostacyclin release could be a potentially useful approach for regeneration of dental pulp.

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Section: Discussionsupporting

confidence: 91%

Section: Originalmentioning

confidence: 99%

Prolonged release of iloprost enhances pulpal blood flow and dentin bridge formation in a rat model of mechanical tooth pulp exposure

et al. 2019

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“…During pulp inflammation, AA metabolism generates prostaglandin E 2 (PGE 2 ) (5) and this lipid mediator produced by stem cells shows such a potent immunomodulator with anti-inflammatory effects through regulation of cell maturation and activation (6). PGE 2 have demonstrate a dual role in proliferation and cell differentiation, because inhibit osteogenic differentiation of mesenchymal stem cells (7), but also has anabolic effects on osteogenic and odontogenic markers in vitro, enhances tertiary dentin formation in vivo (8). These findings shed light on this mediator as a potential therapeutical agent to accelerate pulp repair.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

et al. 2019

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Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells.

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“…On the other hand, studies have shown that PGE 2 inhibits the osteogenic differentiation of mesenchymal stem cells (Ern et al, 2017). In addition, PGE 2 induces the expression of genes associated with mineralization by undifferentiated cells of the dental pulp (Lorencetti‐Silva et al, 2019), improving the formation of tertiary dentine (Limjeerajarus et al, 2014). Various studies carried out on human dental pulps have reported that, during pulp inflammation, the concentration of PGE 2 in dental pulps with pulpitis (Nakanishi et al, 1995) significantly increases, both reversible (Cohen et al, 1985; Petrini et al, 2012) and irreversible (Cohen et al, 1985), compared to healthy dental pulps.…”

Section: Discussionmentioning

confidence: 99%

Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice

et al. 2021

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Aim To evaluate whether treatment with resiniferatoxin (RTX) is capable of lowering the plasma levels of PGE2 and TNF‐α, as well as histopathological parameters in inflammation of pulp tissue in a mouse experimental model. Methodology Ten groups of six BALB/c mice were formed as follows: healthy group (HC), healthy group treated with RTX (HRTX), two groups with pulp inflammation at 14 and 18 hours (PI14/PI18), six groups with pulpal inflammation plus treatment with Ibuprofen (IBU14/IBU18), dexamethasone (DEX14/DEX18) and resiniferatoxin (RTX14/RTX18) at 14 and 18 hours, respectively. Pulpal inflammation was induced through occlusal exposure of the pulp of the maxillary first molar. The plasma levels of PGE2 and TNF‐α and the histological parameters of the pulp tissue of the HC and HRTX groups were evaluated at the time of acquiring the animals. In the other groups, the plasma levels of PGE2 and TNF‐α and the histopathological parameters were evaluated at 14 and 18 hours after pulp damage. Plasma levels of PGE2 and TNF‐α were quantified by ELISA, and the histopathological parameters were evaluated by H/E staining. Statistical significance was determined by one‐way analysis of variance (ANOVA) to test for overall differences between group means. Results A significant increase (*p < .05) in plasma levels of PGE2 and TNF‐α occurred 14 and 18 hours after pulp damage. In addition, treatment with RTX significantly decreased (*p < .05) the plasma levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage, as well as the infiltrate of inflammatory cells at 18 hours after pulp damage, similarly to treatment with ibuprofen and dexamethasone. Conclusion It was possible to detect systemic levels of PGE2 and TNF‐α at 14 and 18 hours after pulp damage. Likewise, treatment with RTX was associated with an anti‐inflammatory effect similar to treatment with ibuprofen and dexamethasone. These findings place resiniferatoxin as a therapeutic alternative in the treatment of inflammatory diseases in Dentistry.

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Iloprost Induces Tertiary Dentin Formation

Cited by 16 publications

References 46 publications

Prolonged release of iloprost enhances pulpal blood flow and dentin bridge formation in a rat model of mechanical tooth pulp exposure

Prolonged release of iloprost enhances pulpal blood flow and dentin bridge formation in a rat model of mechanical tooth pulp exposure

Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

Effect of treatment with resiniferatoxin in an experimental model of pulpal inflammatory in mice

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