Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

Komatsu, Takeshi; Mohammadi, Saeed; Busa, Lori Shayne Alamo; Maeki, Masatoshi; Ishida, Akihiko; Tani, Hirofumi; Tokeshi, Manabu

doi:10.1039/c6an01409g

Cited by 61 publications

(37 citation statements)

References 25 publications

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“…The tomato fruit pericarp colors were measured with a colorimeter using the CIE L*a*b color system (Komatsu et al, 2016). The a* value refers to the degree of red to green, determined by the degradation of chlorophyll and the accumulation of carotenoids, such as b-carotene and lycopene, producing the characteristic yellow and red coloration (Luo et al, 2013).…”

Section: Difference In Phenotype Between Rin-mc-silenced and Rin Fruitmentioning

confidence: 99%

“…For A, fruits were picked at IM, BK, BK+3, BK+5, and BK+10 stages. A hand-held colorimeter with the CIE L*a*b color system was used for pericarp color assay, and a* represents red to green (Komatsu et al, 2016). At least nine replicates were included for each assay.…”

Section: Difference In Phenotype Between Rin-mc-silenced and Rin Fruitmentioning

confidence: 99%

“…A hand-held colorimeter (CR-10 Plus) with the CIE L*a*b color system was chosen for pericarp color assay (Komatsu et al, 2016). The firmness of the pericarp was assayed using a hand-held penetrometer (model FT327 made in Italy) according to the manufacturer's instructions.…”

Section: Color and Firmness Measurementmentioning

confidence: 99%

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The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

et al. 2017

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Fruit development and ripening is regulated by genetic and environmental factors and is of critical importance for seed dispersal, reproduction, and fruit quality. Tomato () () mutant fruit have a classic ripening-inhibited phenotype, which is attributed to a genomic DNA deletion resulting in the fusion of two truncated transcription factors, and In wild-type fruit, RIN, a MADS-box transcription factor, is a key regulator of the ripening gene expression network, with hundreds of gene targets controlling changes in color, flavor, texture, and taste during tomato fruit ripening; , on the other hand, has low expression in fruit, and the potential functions of the fusion gene in ripening remain unclear. Here, overexpression of in transgenic wild-type cv Ailsa Craig tomato fruits impaired several ripening processes, and down-regulating expression in the mutant was found to stimulate the normal yellow mutant fruit to produce a weak red color, suggesting a distinct negative role for in tomato fruit ripening. By comparative transcriptome analysis of and:: RNA interference fruits, a total of 1,168 and 1,234 genes were identified as potential targets of RIN-MC activation and inhibition. Furthermore, the fusion gene was shown to be translated into a chimeric transcription factor that was localized to the nucleus and was capable of protein interactions with other MADS-box factors. These results indicated that tomato RIN-MC fusion plays a negative role in ripening and encodes a chimeric transcription factor that modulates the expression of many ripening genes, thereby contributing to the mutant phenotype.

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Section: Difference In Phenotype Between Rin-mc-silenced and Rin Fruitmentioning

confidence: 99%

Section: Difference In Phenotype Between Rin-mc-silenced and Rin Fruitmentioning

confidence: 99%

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The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

et al. 2017

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“…The μPADs were fabricated by photolithography and screen printing, as described in our previous reports. 12,19,28,29 The μPADs were also fabricated by a wax printer (Xerox Tektronix PHASER 850, Xerox Corp., Tokyo, Japan) and a commercially available craft cutting machine (Silhouette CAMEO, GRAPHTEC Corp.), for a wax printing method and a cutting method, respectively. The detailed fabrication procedures are described in Supporting Information.…”

Section: Pattern Designs and Fabrication Procedures Of μPadsmentioning

confidence: 99%

“…We carried out the colorimetric protein assay in triplicate, and compared the μPADs using the K-scale (color scale) 29 and standard deviation (STD) of color scale. Figure 3(a) shows photographs of μPADs used for the colorimetry.…”

Section: Comparison Of Performance For Protein Assay Using the μPadsmentioning

confidence: 99%

Characteristics of Microfluidic Paper-based Analytical Devices Fabricated by Four Different Methods

et al. 2018

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Engineering Strategies for Advancing Optical Signal Outputs in Smartphone‐Enabled Point‐of‐Care Diagnostics

Fan

Liu

Miao

et al. 2023

Advanced Intelligent Systems

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The use of smartphone‐based analysis systems has been increasing over the past few decades. Among the important reasons for its popularity are its ubiquity, increasing computing power, relatively low cost, and capability to acquire and process data simultaneously in a point‐of‐need fashion. Furthermore, smartphones are equipped with various sensors, especially a complementary metal–oxide–semiconductor (CMOS) sensor. The high sensitivity of the CMOS sensor allows smartphones to be used as a colorimeter, fluorimeter, and spectrometer, constituting the essential part of point‐of‐care testing contributing to E‐health and beyond. However, despite its myriads of merits, smartphone‐based diagnostic devices still face many challenges, including high susceptibility to illumination conditions, difficulty in adapter uniformization, low interphone repeatability, and et al. These problems may hinder smartphone‐enabled diagnosis from passing the FDA regulations of medical devices. This review discusses the design and application of current smartphone‐based diagnostic devices and highlights challenges associated with existent methods and perspectives on how to deal with those challenges from engineering aspects on constant color signal acquisition, including smartphone adapter design, color space transformation, machine learning classification, and color correction.

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Image analysis for a microfluidic paper-based analytical device using the CIE Lab* color system

Cited by 61 publications

References 25 publications

The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

The RIN-MC Fusion of MADS-Box Transcription Factors Has Transcriptional Activity and Modulates Expression of Many Ripening Genes

Characteristics of Microfluidic Paper-based Analytical Devices Fabricated by Four Different Methods

Engineering Strategies for Advancing Optical Signal Outputs in Smartphone‐Enabled Point‐of‐Care Diagnostics

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