Imaging and Manipulation of Plasma Membrane Fatty Acid Clusters Using TOF-SIMS Combined Optogenetics

Zhang, Chi; Kikushima, Kenji; Endo, Mizuki; Kahyo, Tomoaki; Horikawa, Makoto; Matsudaira, Takaomi; Tanaka, Tatsuya; Takanashi, Yusuke; Sato, Tomohito; Takahashi, Yutaka; Xu, Lili; Takayama, Naoki; Islam, Ariful; Mamun, Md. Al; Ozawa, Toshio; Setou, Mitsutoshi

doi:10.3390/cells12010010

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…Lipids play a critical role in various biological processes, including signaling, energy storage, and membrane structure. ToF-SIMS emerged as a powerful technique for identifying and quantifying lipids in biological samples, including brain ( Amaya et al, 2007 ), skin ( Kezutyte et al, 2013 ), liver ( Debois et al, 2009 ), heart ( Sämfors et al, 2019 ) etc., related to various research fields such as tumor microenvironment, pathophysiology of traumatic brain injury, and cellular membrane lipid composition ( Bich et al, 2014a ; Bich et al, 2014b ; Sämfors and Fletcher, 2020 ; Li et al, 2023 ; Zhang et al, 2023 ).…”

Section: Bioimaging Applications In Tof-simsmentioning

confidence: 99%

Advancements in ToF-SIMS imaging for life sciences

Jia,

Zhao,

Zhao

2023

Front. Chem.

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In the last 2 decades, Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) has gained significant prominence as a powerful imaging technique in the field of life sciences. This comprehensive review provides an in-depth overview of recent advancements in ToF-SIMS instrument technology and its applications in metabolomics, lipidomics, and single-cell analysis. We highlight the use of ToF-SIMS imaging for studying lipid distribution, composition, and interactions in cells and tissues, and discuss its application in metabolomics, including the analysis of metabolic pathways. Furthermore, we review recent progress in single-cell analysis using ToF-SIMS, focusing on sample preparation techniques, in situ investigation for subcellular distribution of drugs, and interactions between drug molecules and biological targets. The high spatial resolution and potential for multimodal analysis of ToF-SIMS make it a promising tool for unraveling the complex molecular landscape of biological systems. We also discuss future prospects and potential advancements of ToF-SIMS in the research of life sciences, with the expectation of a significant impact in the field.

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Section: Bioimaging Applications In Tof-simsmentioning

confidence: 99%

Advancements in ToF-SIMS imaging for life sciences

Jia,

Zhao,

Zhao

2023

Front. Chem.

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Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies

Yang,

Yuan,

et al. 2024

Anal Bioanal Chem

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Central nervous system complications in SARS-CoV-2-infected patients

Lin

et al. 2023

J Neurol

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Objective To investigate the clinical manifestations, treatment and prognosis of COVID-19-associated central nervous system (CNS) complications. Methods In this single-centre observation study, we recruited patients with COVID-19-associated CNS complications at the neurology inpatient department of the Eighth Affiliated Hospital, Sun Yat-Sen University (Futian, Shenzhen) from Dec 2022 to Feb 2023. Patients were analysed for demographics, clinical manifestations, cerebrospinal fluid properties, electroencephalographic features, neuroimaging characteristics, and treatment outcome. All patients were followed-up at 1 and 2 months after discharge until Apr 2023. Results Of the 12 patients with COVID-19-associated CNS complications, the CNS symptoms occur between 0 days and 4 weeks after SARS-CoV-2 infection. The most common CNS symptoms were memory deficits (4/12, 33%), Unresponsiveness (4/12, 33%), mental and behavioural disorders (4/12, 33%). Seven of 12 cases can be categorized as probable SARS-CoV-2 encephalitis, and 5 cases can be described as brainstem encephalitis, acute disseminated encephalomyelitis, optic neuritis, multiple sclerosis or tremor probably associated with SARS-CoV-2 infection. Six patients received antiviral therapy, and 11 patients received glucocorticoid therapy, of which 3 patients received human immunoglobulin synchronously. Nine patients recovered well, two patients had residual neurological dysfunction, and one patient passed away from complications associated with tumor. Conclusion In this observational study, we found that the inflammatory or immune-related complications were relatively common manifestations of COVID-19-associated CNS complications, including different phenotypes of encephalitis and CNS inflammatory demyelinating diseases. Most patients recovered well, but a few patients had significant neurological dysfunctions remaining.

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Imaging and Manipulation of Plasma Membrane Fatty Acid Clusters Using TOF-SIMS Combined Optogenetics

Cited by 3 publications

References 63 publications

Advancements in ToF-SIMS imaging for life sciences

Advancements in ToF-SIMS imaging for life sciences

Sample preparation for fatty acid analysis in biological samples with mass spectrometry-based strategies

Central nervous system complications in SARS-CoV-2-infected patients

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