Imaging and Therapeutic Applications of Optical and Thermal Response of SPION-Based Third Generation Plasmonic Nanodendrimers

Abstract

In this study, 9 nm superparamagnetic iron oxide nanoparticles (SPION) were functionalized by polyamidoamine (PAMAM) dendrimer. Using tetracholoroauric acid (HAuCl 4 ), magnetodendrimer (MD) samples were conjugated by gold nanoparticles (Au-NPs). Two different reducing agents, i.e. sodium borohydride and hydrazine sulfate, and pre-synthesized 10-nm Au-NP were used to evaluate the efficiency of conjugation method. The samples were characterized using X-ray diffractometry (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, UV-visible spectroscopy and fluorescence spectroscopy. The results confirmed that AuNPs produced by sodium borohydrate and the pre-synthesized 10-nm Au-NPs were capped by MDs whereas the Au-NP prepared by hydrazine sulfate as a reducing agent was entrapped by MDs. Optical properties of the MDs were studied by laser-induced fluorescence spectroscopy (LIF) within a wide range of visible spectrum. Also, based on the thermal analysis, all synthesized nanostructures exhibited a temperature increase using 488 nm and 514 nm wavelengths of a tunable argon laser. The new iron oxide-dendrimer-Au NPs synthesized by sodium borohydrate (IDANaBH4) produced the highest temperature increase at 488 nm whereas the other nanostructures particularly pure Au-NPs produced more heating effect at 514 nm. These findings suggest the potential application of these nanocomposites in the field of bioimaging, targeted drug delivery and controlled hyperthermia. M. Tajabadi et al.213

Multifunctional Nanoplatform for Targeted Laser-induced Hyperthermia and Microscopy of Breast Cancer Cells using SPION-based Gold and Folic Acid Conjugated Nanodendrimers: An in vitro Assay

“…In our recent report [48], we indicated that all samples have a unique absorption peak in the range of 520-540 nm which reveals the formation of AuNPs. However, other peaks at 280 nm and 320 nm also correspond to Au-NPs aggregation [49,50].…”

Synthesis, Characterization and Imaging of Fluorescine Isothiocyanate Conjugated Magnetite Nanoparticles in MCF 7 Breast Cancer Cell Lines

“…In our recent report, it was shown that the emission of FITC intensity is pH-dependent which can be considered as an advantage since tumors or infl amed tissues are relatively acidic, about 6.5, due to hypoxic conditions [25] thus, the fl uorescence intensity can be used as an easy monitoring probe. Also, it was recently reported by our group that SPION-based plasmonic nanodendrimers can have the potential capability to be utilized for thermal and bioimaging applications [26,27]. In this research, the results of synthesized SPIONs functionalized by fl uorescine isothiocynate (FITC) used as biomarker for fl uorescence imaging of L929 and MCF 7 cancer cell lines are described.…”

Effect of Laser Wavelengths on Drug Release with and without Gold Nanoshells and Magnetic Guidance on Uptake by Cancer Cells

“…The former approach is more feasible due to the availability of hydrophilic layers, however, in order to produce sufficient temperature increase to destabilize the lipid layer to release the liposome content, large amount of MNPs should be used It is suggested by Liao et al [13] that Cetuximab-immunomicelles are a useful delivery vehicles for Dox and SPIO to epidermal growth factor receptor overexpressing tumor cells in-vivo and that it can serve as MRI-visible and targeted drug delivery. In some applications, MNPs are coated by gold layer to form a gold nanoshell (AuNS) for heating purpose rather than guidance [14][15][16]. AuNSs are very promising structures, which exhibit unique optical properties such as strong localized surface plasmon resonance (LSPR), surface-enhanced scattering, nonlinear optical properties, tunable resonance across the Vis-NIR due to adjustable nanoparticle size and shape [17][18][19][20][21].…”