Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy

Lin, Po‐Yen; Lyu, Hong Chou; Hsu, Chin‐Ying Stephen; Chang, Chia Seng; Kao, Fu Jen

doi:10.1364/boe.2.000149

Cited by 35 publications

(34 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…44,45 Lin et al 46 used multimodal (second harmonic, auto-°uorescence, and°uorescence lifetime) method with unlabeled analysis to di®erentiate the morphological characteristics of dentin and enamel (Fig. 11).…”

Section: Oral and Bone Diseasesmentioning

confidence: 99%

“…Wang et al 47 believe that FLIM will be used from the laboratory to the clinic for real-time histology and dynamic monitoring of human liver disease in the near future. In 1999, Mizeret et al 48 developed the video frequency domain FLIM in vivo and applied it in 46 (Permission has been granted by \Biomedical Optics Express".) the¯eld of medical diagnosis (Fig.…”

Section: Vascular and Cavity Organ Diseasesmentioning

confidence: 99%

See 1 more Smart Citation

Applications of fluorescence lifetime imaging in clinical medicine

Wang

Zheng

Zhao

et al. 2017

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

Fluorescence lifetime is not only associated with the molecular structure of fluorophores, but also strongly depends on the environment around them, which allows fluorescence lifetime imaging microscopy (FLIM) to be used as a tool for precise measurement of the cell or tissue microenvironment. This review introduces the basic principle of fluorescence lifetime imaging technology and its application in clinical medicine, including research and diagnosis of diseases in skin, brain, eyes, mouth, bone, blood vessels and cavity organs, and drug evaluation. As a noninvasive, nontoxic and nonionizing radiation technique, FLIM demonstrates excellent performance with high sensitivity and specificity, which allows to determine precise position of the lesion and, thus, has good potential for application in biomedical research and clinical diagnosis.

show abstract

Section: Oral and Bone Diseasesmentioning

confidence: 99%

Section: Vascular and Cavity Organ Diseasesmentioning

confidence: 99%

Applications of fluorescence lifetime imaging in clinical medicine

Wang

Zheng

Zhao

et al. 2017

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

show abstract

“…Therefore, various techniques have been attempted to highlight carious lesions and to distinguish these histological zones [26][27][28][29] .…”

Section: Introductionmentioning

confidence: 99%

Observation of Carious Lesions on Undecalcified Tooth Sections with Silver Staining Method for Protein Gel Electrophoresis

Kuwada-Kusunose

Suzuki

Fusé³

et al. 2016

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Previously, we indicated that a silver staining method generally used in protein gel electrophoresis could be applied to stain undecalcified tooth sections for observation of fine structures in dentin and cementum. In this study, we attempted to observe the carious lesions of enamel and dentin with the silver staining method. When undecalcified ground sections of teeth were stained with the staining method, carious lesions on dentin showed layer staining pattern, and the staining patterns seemed to be corresponding to the histological zone of the carious lesions. Furthermore, it was clear that discoloration of tooth sections stained by silver was promoted by UV light exposure, as a result, the enamel carious lesions could be observed. Our results suggested an application possibility of the silver staining to detect the carious lesions in both dentin and enamel.

show abstract

“…Multiphoton tomography not only allows for the differential use of excitation wavelengths and spectral emission analysis, but also for picosecond‐resolved fluorescence lifetime measurements (FLIM). In medical research, the FLIM technique was applied amongst others for the imaging of cutaneous tumours (19,20), cellular skin fluorophores (21), artherosclerotic plaques (22), dental tissues (23) and cornea (24).…”

Section: Introductionmentioning

confidence: 99%

Non‐invasive evaluation of dermal elastosis by in vivo multiphoton tomography with autofluorescence lifetime measurements

Koehler

Preller

Elsner

et al. 2011

Experimental Dermatology

View full text Add to dashboard Cite

The non-invasive differentiation of dermal elastic fibres from solar elastosis in vivo is of great interest in dermatologic research, especially for efficacy testing of anti-ageing products. To date, no studies on multiphoton excited fluorescence lifetime characteristics of human elastic fibres and solar elastosis are reported. The goal of the present work was the identification of differential criteria for elastic fibres and solar elastosis by the analysis of fluorescence decay curves acquired by time-correlated single photon counting in vivo multiphoton tomography. For this purpose, fluorescence lifetime measurements (FLIM) were performed with 47 volunteers of different age groups at sun-protected and sun-exposed localizations. Bi-exponential curve fitting was applied to the FLIM data, and characteristic differences between age groups and localizations were found in both relevant fit parameters describing the decay slope. The FLIM analyses have shown that dermal autofluorescence has different lifetimes depending on age and in part on localization.

show abstract

Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy

Cited by 35 publications

References 33 publications

Applications of fluorescence lifetime imaging in clinical medicine

Applications of fluorescence lifetime imaging in clinical medicine

Observation of Carious Lesions on Undecalcified Tooth Sections with Silver Staining Method for Protein Gel Electrophoresis

Non‐invasive evaluation of dermal elastosis by in vivo multiphoton tomography with autofluorescence lifetime measurements

Contact Info

Product

Resources

About