“…For the last three years, several groups have developed new tissue clearing methods for enhanced imaging of entire tissues: ClearT (Kuwajima et al, 2013), SeeDB (Ke et al, 2013;Ke and Imai, 2014), ScaleA (Hama et al, 2011), CLARITY Tomer et al, 2014), PACT/PARS , CU-BIC Tainaka et al, 2014), and 3-dimensional imaging of solvent cleared organ (3DISCO) (Erturk and Bradke, 2013;Erturk et al, 2014). When combined with subsequent lightsheet laser scanning microscopy (Dodt et al, 2007), 3DISCO, offers the possibility of imaging entire transparent organs without the need for sectioning (Dodt et al, 2007;Erturk et al, 2012;Erturk and Bradke, 2013).…”