Imaging collagen packing dynamics during mineralization of engineered bone tissue

Campi, Gaetano; Fratini, Michela; Bukreeva, Inna; Ciasca, Gabriele; Burghammer, Manfred; Brun, Francesco; Tromba, Giuliana; Mastrogiacomo, M.; Cedola, Alessia

doi:10.1016/j.actbio.2015.05.033

Cited by 35 publications

(33 citation statements)

References 49 publications

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“…This makes standard experimental probes probing the average structure inadequate for the visualization of this heterogeneity, requiring highly spatially resolved probes. Biological tissues are typically intrinsically heterogeneous; indeed, nowadays new features and properties have been visualized using scanning methods with high spatial resolutions, such as by Atomic Force Microscopy [38][39][40], Confocal Microscopy [40], Scanning Electron Microscopy [35] and Scanning micro X-ray diffraction [15][16][17][18][19][20][21]. Correlated spatial structural fluctuations in biological systems have been correlated with the emergence of quantum coherence in biological matter [35,41], in photosystems [42] and intrinsically disordered proteins [43,44], as well as in lamellar oxides showing quantum coherence [45][46][47][48], where non-Euclidean spatial geometries for signal transmission are able to emerge from a correlated disorder [22,48].…”

Section: Discussionmentioning

confidence: 99%

“…We measured axon myelin orientation point by point, using a synchrotron radiation X-ray beam focused to micrometric dimensions at the ID 13 beamline at the European synchrotron radiation facility, ESRF, in Grenoble [15][16][17][18][19][20][21]. Afterwards, we applied statistical physics tools to the big data set of diffraction patterns collected to unveil the "statistical distributions of the fluctuating myelin angular order".…”

Section: Introductionmentioning

confidence: 99%

“…Here, we have investigated the spatial distribution of the myelin orientation order in a nerve using the novel technique scanning micro X-ray diffraction (SµXRD) [15][16][17][18][19][20][21][22], which allows us to probe both the real space and the k-space with high resolution.…”

Section: Introductionmentioning

confidence: 99%

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Correlated Disorder in Myelinated Axons Orientational Geometry and Structure

et al. 2017

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While the ultrastructure of myelin is considered a quasi-crystalline stable system, nowadays its multiscale complex dynamics appear to play a key role in its functionality, degeneration and repair processes following neurological diseases and trauma. In this work, we investigated the fluctuation of the myelin supramolecular assembly by measuring the spatial distribution of orientation fluctuations of axons in a Xenopus Laevis sciatic nerve associated with nerve functionality. To this end, we used scanning micro X-ray diffraction (SµXRD), a non-invasive technique that has already been applied to other heterogeneous systems presenting complex geometries from microscale to nanoscale. We found that the orientation of the spatial fluctuations of fresh axons show a Levy flight distribution, which is a clear indication of correlated disorder. We found that the Levy flight distribution was missing in the aged nerve prepared in an unfresh state. This result shows that the spatial distribution of axon orientation fluctuations in unfresh nerve state loses the correlated disorder and assumes a random disorder behavior. This work provides a deeper understanding of the ultrastructure-function nerve relation and paves the way for the study of other materials and biomaterials using the SµXRD technique to detect fluctuations in their supramolecular structure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Correlated Disorder in Myelinated Axons Orientational Geometry and Structure

et al. 2017

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“…Collagen fibers are known to align spontaneously; several studies have reported the liquid crystalline character of collagen solutions depending on the concentration [2,3]. The lateral spacing and orientation of collagen fibrils during the anisotropic growth of mineral nanocrystals has also been visualized [4]. Importantly, bone tissue orientation, rather than bone mass, is the dominant factor controlling the mechanical function of bone [5].…”

Section: Introductionmentioning

confidence: 99%

Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation

Matsugaki

Nakano

2016

Crystals

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Abstract:The anisotropic microstructure of bone tissue is crucial for appropriate mechanical and biological functions of bone. We recently revealed that the construction of oriented bone matrix is established by osteoblast alignment; there is a quite unique correlation between cell alignment and cell-produced bone matrix orientation governed by the molecular interactions between material surface and cells. Titanium and its alloys are one of the most attractive materials for biomedical applications. We previously succeeded in controlling cellular arrangement using the dislocations of a crystallographic slip system in titanium single crystals with hexagonal close-packing (hcp) crystal lattice. Here, we induced a specific surface topography by deformation twinning and dislocation motion to control cell orientation. Dislocation and deformation twinning were introduced into α-titanium polycrystals in compression, inducing a characteristic surface structure involving nanometer-scale highly concentrated twinning traces. The plastic deformation-induced surface topography strongly influenced osteoblast orientation, causing them to align preferentially along the slip and twinning traces. This surface morphology, exhibiting a characteristic grating structure, controlled the localization of focal adhesions and subsequent elongation of stress fibers in osteoblasts. These results indicate that cellular responses against dislocation and deformation twinning are useful for controlling osteoblast alignment and the resulting bone matrix anisotropy.

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“…The emergence of multiscale patterns, from the microscale down to the nanoscale, gives new physical-chemical properties in several class of materials belonging to different research field such as chemistry [1,2], material engineering [3] and biomedicine [4][5][6], just to mention a few.…”

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confidence: 99%