Imaging in vivo dynamics of sensory axon responses to CNS injury

Schaffran, Barbara; Hilton, Brett J.; Bradke, Frank

doi:10.1016/j.expneurol.2019.02.010

Cited by 6 publications

(7 citation statements)

References 74 publications

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“…Conditioning also enhanced growth cone dynamics in vivo. We transected single dorsal column (DC) axons using a localized laser lesion at the L1 level of the spinal cord in naive or conditioned GFP-M mice as described before (Figure 1E) (Schaffran et al, 2019;Ylera et al, 2009). The dynamics of the proximal stump from injured axons were studied within the first 4.5 h after lesion using two-photon time-lapse microscopy (Figures 1F and 1G).…”

Section: Rapid Actin Turnover Is Essential For Accelerated Growth After Conditioningmentioning

confidence: 99%

ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS

Tedeschi

Dupraz

Curcio

et al. 2019

Neuron

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Section: Rapid Actin Turnover Is Essential For Accelerated Growth After Conditioningmentioning

confidence: 99%

ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS

Tedeschi

Dupraz

Curcio

et al. 2019

Neuron

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“…(Kerschensteiner et al ., 2005; Odoardi et al ., 2007; Johannssen & Helmchen, 2010; Kim et al ., 2010; Nikić et al ., 2011; Davalos & Akassoglou, 2012; Farrar et al ., 2012; Fenrich et al ., 2013; Coisne et al ., 2013; Evans et al ., 2014; Schaffran et al ., 2019),…”

Section: Animal Restraint and Tissue Immobilizationmentioning

confidence: 99%

“…As physical restraint of the spinal cord itself is not possible, vertebrae can be targeted to dampen its movement. For example, some studies described a stabilization method for short‐term imaging in which mice are suspended with spinal clamps to elevate the abdomen and allow them to breath freely (Johannssen & Helmchen, 2010; Nikić et al ., 2011; Coisne et al ., 2013; Schaffran et al ., 2019). Since residual motion artefacts caused by heartbeat and breathing are generally not eliminated altogether, they can be further reduced by embedding the spinal cord in agar (Kerschensteiner et al ., 2005).…”

Section: Animal Restraint and Tissue Immobilizationmentioning

confidence: 99%

Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions

Soulet

Lamontagne‐Proulx

Aubé

et al. 2020

Journal of Microscopy

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Summary Since its invention 29 years ago, two‐photon laser‐scanning microscopy has evolved from a promising imaging technique, to an established widely available imaging modality used throughout the biomedical research community. The establishment of two‐photon microscopy as the preferred method for imaging fluorescently labelled cells and structures in living animals can be attributed to the biophysical mechanism by which the generation of fluorescence is accomplished. The use of powerful lasers capable of delivering infrared light pulses within femtosecond intervals, facilitates the nonlinear excitation of fluorescent molecules only at the focal plane and determines by objective lens position. This offers numerous benefits for studies of biological samples at high spatial and temporal resolutions with limited photo‐damage and superior tissue penetration. Indeed, these attributes have established two‐photon microscopy as the ideal method for live‐animal imaging in several areas of biology and have led to a whole new field of study dedicated to imaging biological phenomena in intact tissues and living organisms. However, despite its appealing features, two‐photon intravital microscopy is inherently limited by tissue motion from heartbeat, respiratory cycles, peristalsis, muscle/vascular tone and physiological functions that change tissue geometry. Because these movements impede temporal and spatial resolution, they must be properly addressed to harness the full potential of two‐photon intravital microscopy and enable accurate data analysis and interpretation. In addition, the sources and features of these motion artefacts are varied, sometimes unpredictable and unique to specific organs and multiple complex strategies have previously been devised to address them. This review will discuss these motion artefacts requirement and technical solutions for their correction and after intravital two‐photon microscopy.

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“…Because of the unique relation between the functionality of a single M-axon and the occurrence of short-latency escapes 32 it should even be possible to follow the regeneration of the M-axon while in parallel observing shortlatency escapes to probe the time course of functional recoverythus linking functional recovery with the regeneration of a single axon. Precisely targeted and M-axon specific injury was achieved by using the GFP labelled M-cells of our Ca-Tol-056 line and a two-photon laser 42 . First, we confirmed the regenerative capacity of the M-axon for injury in approximately 500 μm (495 ± 17 μm; N = 16) distance from the M-soma (Fig.…”

Section: Proximity Of Injury Site To the Soma Determines Regenerationmentioning

confidence: 99%

High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish

Hecker

Anger

et al. 2020

Commun Biol

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In non-mammalian vertebrates, some neurons can regenerate after spinal cord injury. One of these, the giant Mauthner (M-) neuron shows a uniquely direct link to a robust survivalcritical escape behavior but appears to regenerate poorly. Here we use two-photon microscopy in parallel with behavioral assays in zebrafish to show that the M-axon can regenerate very rapidly and that the recovery of functionality lags by just days. However, we also find that the site of the injury is critical: While regeneration is poor both close and far from the soma, rapid regeneration and recovery of function occurs for injuries between 10% and 50% of total axon length. Our findings show that rapid regeneration and the recovery of function can be studied at remarkable temporal resolution after targeted injury of one single M-axon and that the decision between poor and rapid regeneration can be studied in this one axon.

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Imaging in vivo dynamics of sensory axon responses to CNS injury

Cited by 6 publications

References 74 publications

ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS

ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS

Multiphoton intravital microscopy in small animals: motion artefact challenges and technical solutions

High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish

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