Imaging margins of skin tumors using laser-induced breakdown spectroscopy and machine learning

Kiss, Kateřina; Šindelářová, Anna; Krbal, Lukáš; Stejskal, Václav; Mrázová, Kristýna; Vrábel, Jakub; Kaška, M; Modlitbová, Pavlína; Pořízka, Pavel; Kaiser, Jozef

doi:10.1039/d0ja00469c

Cited by 40 publications

(20 citation statements)

References 34 publications

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“…The LIBS technique has been employed for chemical imaging with element-specific spatial contrast (''element imaging'') of many different materials 1 including biological samples, [2][3][4] ceramics, 5 metal coatings, 6 metals, [7][8][9][10][11] nanoparticles, 12,13 Li-ion solid-state electrolytes, 14,15 printed circuit boards, 16 and thin films. 17 The achievable spatial resolution depends on the size of the ablation crater.…”

Section: Introductionmentioning

confidence: 99%

Femtosecond Single-Pulse and Orthogonal Double-Pulse Laser-Induced Breakdown Spectroscopy (LIBS): Femtogram Mass Detection and Chemical Imaging with Micrometer Spatial Resolution

et al. 2021

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Femtosecond laser-induced breakdown spectroscopy (fs-LIBS) is employed to detect tiny amounts of mass ablated from macroscopic specimens and to measure chemical images of microstructured samples with high spatial resolution. Frequency-doubled fs-pulses (length 400 fs, wavelength 520 nm) are tightly focused with a Schwarzschild microscope objective to ablate the sample surface. The optical emission of laser-induced plasma (LIP) is collected by the objective and measured with an echelle spectrometer equipped with an intensified charge-coupled device camera. A second fs-laser pulse (1040 nm) in orthogonal beam arrangement is reheating the LIP. The optimization of the experimental setup and measurement parameters enables us to record single-pulse fs-LIBS spectra of 5 nm thin metal layers with an ablated mass per pulse of 100 femtogram (fg) for Cu and 370 fg for Ag films. The orthogonal double-pulse fs-LIBS enhances the recorded emission line intensities (two to three times) and improves the contrast of chemical images in comparison to single-pulse measurements. The size of ablation craters (diameters as small as 1.5 µm) is not increased by the second laser pulse. The combination of minimally invasive sampling by a tightly focused low-energy fs-pulse and of strong enhancement of plasma emission by an orthogonal high-energy fs-pulse appears promising for future LIBS chemical imaging with high spatial resolution and with high spectrochemical sensitivity.

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Section: Introductionmentioning

confidence: 99%

Femtosecond Single-Pulse and Orthogonal Double-Pulse Laser-Induced Breakdown Spectroscopy (LIBS): Femtogram Mass Detection and Chemical Imaging with Micrometer Spatial Resolution

et al. 2021

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“…Laser-induced breakdown spectroscopy (LIBS) is an atomic emission spectrometry technique with many advantages such as no or less sample pretreatment, simultaneous multi-element analysis, almost non-destructive detection, and online in situ analysis. 5,6 LIBS has been applied in medicine, [7][8][9] agriculture, 10,11 criminal investigation, [12][13][14] archeology, [15][16][17] and coal analysis. [18][19][20] Unfortunately, its accuracy would be decreased due to matrix differences.…”

Section: Introductionmentioning

confidence: 99%

Correction of moisture interference in laser-induced breakdown spectroscopy detection of coal by combining neural networks and random spectral attenuation

Chen

Liu

et al. 2022

J. Anal. At. Spectrom.

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“…The elevated Ca, Mg, and Na trace elements exist in melanoma lesions, emphasizing the Mg regulating effect on cell division. However, LIBS demonstrates a poor ability to diagnose various malignant tissue types in detail [31][32][33][34][35][36] .…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Normal and Cancerous Human Skin Tissues Based on Laser-Induced Spectral Shift Fluorescence Microscopy

Niazi

Parvin

Jafargholi

et al. 2022

Preprint

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A homemade spectral shift fluorescence microscope (SSFM) is coupled with a spectrometer to record the spectral images of specimens based on the emission wavelength. Here a reliable diagnosis of neoplasia is achieved according to the spectral fluorescence properties of ex-vivo skin tissues after Rd6G staining. It is shown that certain spectral shifts occur for nonmelanoma/melanoma lesions against normal/benign nevus, leading to spectral micrographs. In fact, there is a strong correlation between the emission wavelength and the sort of skin lesions, mainly due to the Rd6G interaction with the mitochondria of cancerous cells. The normal tissues generally enjoy a significant red shift regarding laser line. Conversely, plenty of fluorophores are conjugated to unhealthy cells giving rise to a relative blue shift against normal tissues or a smaller redshift with respect to the excitation wavelength. Consequently, three data sets are available in the form of micrographs, addressing pixel by pixel signal intensity, emission wavelength, and fluorophore concentration of specimens for prompt diagnosis.

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Imaging margins of skin tumors using laser-induced breakdown spectroscopy and machine learning

Abstract: Nowadays, laser-based techniques are play a significant role in medicine, mainly in the ophthalmology, dermatology, and surgical fields. So far, it presents mostly therapeutic applications, although provides considerable potential for...

Cited by 40 publications

References 34 publications

Femtosecond Single-Pulse and Orthogonal Double-Pulse Laser-Induced Breakdown Spectroscopy (LIBS): Femtogram Mass Detection and Chemical Imaging with Micrometer Spatial Resolution

Femtosecond Single-Pulse and Orthogonal Double-Pulse Laser-Induced Breakdown Spectroscopy (LIBS): Femtogram Mass Detection and Chemical Imaging with Micrometer Spatial Resolution

Correction of moisture interference in laser-induced breakdown spectroscopy detection of coal by combining neural networks and random spectral attenuation

Discrimination of Normal and Cancerous Human Skin Tissues Based on Laser-Induced Spectral Shift Fluorescence Microscopy

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