INTRODUCTION:Tissue harmonic imaging (THI) is a new ultrasound imaging technique providing the images which are much superior in quality compare to conventional ultrasound images by improving lateral resolution, signal to noise ratio and reducing side-lobe artifacts. AIM: Our aim is to estimate diagnostic accuracy of THI in diagnosing the cause of obstructive jaundice and to compare it with previous studies. MATERIAL AND METHODS: 125 cases of obstructive jaundice were evaluated with THI for the cause of obstructive jaundice by three radiologists separately during period of two year interval on Phillips HD 11 XE ultrasound scanner. Follow up done with ERCP, surgery and histopathology. CT and MRCP done wherever needed. Sensitivity, specificity, PPV, NPV, accuracy, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio and Youden's index were calculated and compared with previous studies. RESULT: In our study we found choledocholithiasis as most common cause for obstructive jaundice. Sensitivity of 80%, 57.49%, 70.97% and specificity of 90%, 90.59%, 89.36% was noted for choledocholitiasis, neoplasm and benign stricture respectively. Accuracy of 86.4%, 80%, 84.8% was found for choledocholitiasis, neoplasm and benign stricture respectively. PPV of 81.81%, 74.19%, 68.75% and NPV of 88.88%, 81.91%, 90.32% was observed for choledocholithiasis, neoplasm and benign stricture respectively. Positive likelihood ratio of 8, 6.10, 6.67 and negative likelihood ratio of 0.22, 0.46, 0.32 was noted for choledocholithiasis, neoplasm and benign stricture respectively. Diagnostic odds ratio and Youden's index of 36 and 0.7, 13.02 and 0.48, 20.53 and 0.60 was seen for choledocholithiasis, neoplasm and benign stricture respectively. CONCLUSION: Introduction of THI in the modern ultrasound equipment had made improvement in the diagnosis of cause for biliary tract obstruction. Till today even with THI ultrasound doesn't have higher diagnostic value.